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#include "third_party/nucleus/io/bed_reader.h"
#include <utility>
#include <vector>
#include <gmock/gmock-generated-matchers.h>
#include <gmock/gmock-matchers.h>
#include <gmock/gmock-more-matchers.h>
#include "tensorflow/core/platform/test.h"
#include "third_party/nucleus/testing/protocol-buffer-matchers.h"
#include "third_party/nucleus/testing/test_utils.h"
#include "third_party/nucleus/util/utils.h"
namespace nucleus {
using std::vector;
using ::testing::Pointwise;
constexpr char kBedFilename[] = "test_regions.bed";
constexpr char kGzippedBedFilename[] = "test_regions.bed.gz";
constexpr char kMalformedBedFilename[] = "malformed.bed";
constexpr char k5ColumnBedFileName[] = "5col.bed";
class BedReaderTest : public ::testing::Test {
protected:
void SetUp() override {
nucleus::genomics::v1::BedRecord first, second;
first.set_reference_name("chr1");
first.set_start(10);
first.set_end(20);
first.set_name("first");
first.set_score(100);
first.set_strand(nucleus::genomics::v1::BedRecord::FORWARD_STRAND);
first.set_thick_start(12);
first.set_thick_end(18);
first.set_item_rgb("255,124,1");
first.set_block_count(3);
first.set_block_sizes("2,6,2");
first.set_block_starts("10,12,18");
second.set_reference_name("chr1");
second.set_start(100);
second.set_end(200);
second.set_name("second");
second.set_score(250);
second.set_strand(nucleus::genomics::v1::BedRecord::NO_STRAND);
second.set_thick_start(120);
second.set_thick_end(180);
second.set_item_rgb("252,122,12");
second.set_block_count(2);
second.set_block_sizes("35,40");
second.set_block_starts("100,160");
golden_ = {first, second};
}
vector<nucleus::genomics::v1::BedRecord> golden_;
};
TEST_F(BedReaderTest, NormalIterationWorks) {
std::unique_ptr<BedReader> reader =
std::move(BedReader::FromFile(GetTestData(kBedFilename),
nucleus::genomics::v1::BedReaderOptions())
.ValueOrDie());
EXPECT_THAT(as_vector(reader->Iterate()), Pointwise(EqualsProto(), golden_));
}
TEST_F(BedReaderTest, GzippedIterationWorks) {
auto opts = nucleus::genomics::v1::BedReaderOptions();
std::unique_ptr<BedReader> reader = std::move(
BedReader::FromFile(GetTestData(kGzippedBedFilename), opts).ValueOrDie());
EXPECT_THAT(as_vector(reader->Iterate()), Pointwise(EqualsProto(), golden_));
}
TEST_F(BedReaderTest, FieldRestrictionWorks) {
auto opts = nucleus::genomics::v1::BedReaderOptions();
opts.set_num_fields(4);
std::unique_ptr<BedReader> reader = std::move(
BedReader::FromFile(GetTestData(kBedFilename), opts).ValueOrDie());
nucleus::genomics::v1::BedRecord efirst, esecond;
efirst.set_reference_name("chr1");
efirst.set_start(10);
efirst.set_end(20);
efirst.set_name("first");
esecond.set_reference_name("chr1");
esecond.set_start(100);
esecond.set_end(200);
esecond.set_name("second");
vector<nucleus::genomics::v1::BedRecord> expected = {efirst, esecond};
EXPECT_THAT(as_vector(reader->Iterate()), Pointwise(EqualsProto(), expected));
}
TEST_F(BedReaderTest, MalformedBedRecord) {
std::unique_ptr<BedReader> reader =
std::move(BedReader::FromFile(GetTestData(kMalformedBedFilename),
nucleus::genomics::v1::BedReaderOptions())
.ValueOrDie());
EXPECT_DEATH(as_vector(reader->Iterate()),
"BED record has invalid number of fields");
}
TEST_F(BedReaderTest, FiveColBedRecord) {
auto status = BedReader::FromFile(GetTestData(k5ColumnBedFileName),
nucleus::genomics::v1::BedReaderOptions());
// See https://github.com/google/deepvariant/issues/374#issuecomment-723752207
EXPECT_FALSE(status.ok());
}
} // namespace nucleus