[9b26b7]: / deepvariant / testdata / input / realigned_reads.pbtxt

Download this file

18008 lines (17905 with data), 368.4 kB

fragment_name: "HISEQ1:22:H9UJNADXX:1:1110:3389:8225"
proper_placement: true
fragment_length: 394
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091553
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "ATGAATCAGAAACATATGGATTAAATTTCCTTTAAATTGCTCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATA"
aligned_quality: 31
aligned_quality: 31
aligned_quality: 33
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38091799
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:2113:4428:66006"
proper_placement: true
fragment_length: -436
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091555
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "GAATCAGAAACATATGGATTAAATTTCCTTTAAATTGCTCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACT"
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 19
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 31
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 31
aligned_quality: 25
aligned_quality: 34
aligned_quality: 24
aligned_quality: 23
aligned_quality: 34
aligned_quality: 32
aligned_quality: 31
aligned_quality: 36
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 34
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 29
aligned_quality: 29
aligned_quality: 32
aligned_quality: 34
aligned_quality: 31
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 29
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 26
aligned_quality: 33
aligned_quality: 27
aligned_quality: 29
aligned_quality: 32
aligned_quality: 36
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 36
aligned_quality: 38
aligned_quality: 39
aligned_quality: 34
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 36
aligned_quality: 38
aligned_quality: 37
aligned_quality: 30
aligned_quality: 31
aligned_quality: 37
aligned_quality: 35
aligned_quality: 31
aligned_quality: 38
aligned_quality: 36
aligned_quality: 38
aligned_quality: 37
aligned_quality: 39
aligned_quality: 38
aligned_quality: 33
aligned_quality: 32
aligned_quality: 31
aligned_quality: 34
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 36
aligned_quality: 40
aligned_quality: 38
aligned_quality: 36
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 38
aligned_quality: 33
aligned_quality: 39
aligned_quality: 33
aligned_quality: 35
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 35
aligned_quality: 30
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091267
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1114:7406:100504"
proper_placement: true
fragment_length: -569
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091557
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 147
  }
  cigar {
    operation: INSERT
    operation_length: 1
  }
}
aligned_sequence: "ATCAGAAACATATGGATTAAATTTCCTTTAAATTGCTCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGCGCTGAAGATATGAATAGCACCCATACTGC"
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 25
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 30
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 29
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 31
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 34
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 29
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 34
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 36
aligned_quality: 34
aligned_quality: 8
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 19
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 38
aligned_quality: 38
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 25
aligned_quality: 35
aligned_quality: 31
aligned_quality: 31
aligned_quality: 30
next_mate_position {
  reference_name: "20"
  position: 38091136
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:2212:19230:14327"
proper_placement: true
fragment_length: 577
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091559
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 145
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
}
aligned_sequence: "CAGAAACATATGGATTAAATTTCCTTTAAATTGCTCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTG"
aligned_quality: 31
aligned_quality: 34
aligned_quality: 31
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 33
aligned_quality: 39
aligned_quality: 37
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 36
aligned_quality: 39
aligned_quality: 36
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 36
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 36
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 36
aligned_quality: 36
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 36
aligned_quality: 38
aligned_quality: 36
aligned_quality: 35
aligned_quality: 37
aligned_quality: 38
aligned_quality: 38
aligned_quality: 28
aligned_quality: 34
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 33
aligned_quality: 36
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 37
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 28
aligned_quality: 31
aligned_quality: 33
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 29
aligned_quality: 29
aligned_quality: 34
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 30
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 29
aligned_quality: 18
aligned_quality: 31
aligned_quality: 25
next_mate_position {
  reference_name: "20"
  position: 38091988
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:2107:8198:65034"
proper_placement: true
fragment_length: -621
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091567
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TATGGATTAAATTTCCTTTAAATTGCTCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAG"
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 37
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 37
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091094
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:2207:6118:14271"
proper_placement: true
fragment_length: 590
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091572
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "ATTAAATTTCCTTTAAATTGCTCTCAAGATTAAAAATCTAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCA"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 34
aligned_quality: 37
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38092014
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:2212:9778:60821"
proper_placement: true
fragment_length: 571
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091574
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TAAATTTCCTTTAAATTGCTCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGA"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 36
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 29
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
next_mate_position {
  reference_name: "20"
  position: 38091997
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:2114:4019:67696"
proper_placement: true
fragment_length: 366
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091576
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 128
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
}
aligned_sequence: "AATTTCCTTTAAATTGCTCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCT"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 37
aligned_quality: 33
aligned_quality: 37
aligned_quality: 37
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 34
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 29
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 30
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 25
next_mate_position {
  reference_name: "20"
  position: 38091794
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1212:18092:21616"
proper_placement: true
fragment_length: -429
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091576
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AATTTCCTTTAAATTGCTCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGT"
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 37
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091295
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1107:10030:53790"
proper_placement: true
fragment_length: 308
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091576
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AATTTCCTTTAAATTGCTCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGT"
aligned_quality: 30
aligned_quality: 31
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 29
aligned_quality: 35
aligned_quality: 37
aligned_quality: 35
aligned_quality: 37
aligned_quality: 35
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 24
aligned_quality: 19
aligned_quality: 34
aligned_quality: 19
aligned_quality: 37
aligned_quality: 36
aligned_quality: 34
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 35
aligned_quality: 38
aligned_quality: 36
aligned_quality: 24
aligned_quality: 30
aligned_quality: 31
aligned_quality: 27
aligned_quality: 34
aligned_quality: 32
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 34
aligned_quality: 18
aligned_quality: 30
aligned_quality: 33
aligned_quality: 38
aligned_quality: 34
aligned_quality: 38
aligned_quality: 33
aligned_quality: 33
aligned_quality: 38
aligned_quality: 31
aligned_quality: 31
aligned_quality: 27
aligned_quality: 17
aligned_quality: 30
aligned_quality: 31
aligned_quality: 34
aligned_quality: 26
aligned_quality: 35
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 31
aligned_quality: 31
aligned_quality: 34
aligned_quality: 36
aligned_quality: 32
aligned_quality: 23
aligned_quality: 26
aligned_quality: 34
aligned_quality: 28
aligned_quality: 33
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 30
aligned_quality: 28
aligned_quality: 30
aligned_quality: 30
aligned_quality: 34
aligned_quality: 30
aligned_quality: 7
aligned_quality: 20
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 29
aligned_quality: 30
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 32
aligned_quality: 31
aligned_quality: 32
aligned_quality: 25
aligned_quality: 29
aligned_quality: 19
aligned_quality: 31
aligned_quality: 32
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 25
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 31
aligned_quality: 27
aligned_quality: 23
aligned_quality: 29
aligned_quality: 10
aligned_quality: 24
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 29
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 27
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 30
aligned_quality: 30
aligned_quality: 33
aligned_quality: 33
aligned_quality: 30
aligned_quality: 19
next_mate_position {
  reference_name: "20"
  position: 38091736
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1115:18966:3851"
proper_placement: true
fragment_length: 763
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091580
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TCCTTTAAATTGCTCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTA"
aligned_quality: 27
aligned_quality: 31
aligned_quality: 31
aligned_quality: 35
aligned_quality: 10
aligned_quality: 28
aligned_quality: 25
aligned_quality: 28
aligned_quality: 25
aligned_quality: 28
aligned_quality: 34
aligned_quality: 23
aligned_quality: 27
aligned_quality: 37
aligned_quality: 30
aligned_quality: 33
aligned_quality: 37
aligned_quality: 38
aligned_quality: 35
aligned_quality: 39
aligned_quality: 37
aligned_quality: 36
aligned_quality: 27
aligned_quality: 32
aligned_quality: 18
aligned_quality: 27
aligned_quality: 37
aligned_quality: 31
aligned_quality: 34
aligned_quality: 24
aligned_quality: 34
aligned_quality: 37
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 33
aligned_quality: 37
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 15
aligned_quality: 33
aligned_quality: 24
aligned_quality: 30
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 17
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 37
aligned_quality: 31
aligned_quality: 23
aligned_quality: 8
aligned_quality: 28
aligned_quality: 34
aligned_quality: 37
aligned_quality: 40
aligned_quality: 28
aligned_quality: 31
aligned_quality: 29
aligned_quality: 28
aligned_quality: 17
aligned_quality: 33
aligned_quality: 36
aligned_quality: 40
aligned_quality: 36
aligned_quality: 30
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 22
aligned_quality: 21
aligned_quality: 28
aligned_quality: 30
aligned_quality: 32
aligned_quality: 28
aligned_quality: 26
aligned_quality: 32
aligned_quality: 20
aligned_quality: 29
aligned_quality: 26
aligned_quality: 31
aligned_quality: 30
aligned_quality: 12
aligned_quality: 26
aligned_quality: 30
aligned_quality: 25
aligned_quality: 31
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 31
aligned_quality: 32
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 29
aligned_quality: 25
aligned_quality: 31
aligned_quality: 33
aligned_quality: 33
aligned_quality: 31
aligned_quality: 29
aligned_quality: 32
aligned_quality: 35
aligned_quality: 32
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 32
aligned_quality: 29
aligned_quality: 23
aligned_quality: 17
aligned_quality: 23
aligned_quality: 30
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 25
aligned_quality: 33
aligned_quality: 31
aligned_quality: 19
aligned_quality: 10
aligned_quality: 29
aligned_quality: 32
aligned_quality: 33
aligned_quality: 13
aligned_quality: 17
aligned_quality: 19
aligned_quality: 25
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 23
aligned_quality: 30
aligned_quality: 27
aligned_quality: 23
aligned_quality: 30
aligned_quality: 31
aligned_quality: 32
aligned_quality: 32
aligned_quality: 31
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38092195
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:2208:15535:70518"
proper_placement: true
fragment_length: 585
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091581
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "CCTTTAAATTGCTCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTAT"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 37
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38092018
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:2211:18770:91301"
proper_placement: true
fragment_length: 371
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091584
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 120
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
}
aligned_sequence: "TTAAATTGCTCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCA"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 37
aligned_quality: 39
aligned_quality: 34
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 26
aligned_quality: 36
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 32
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 33
aligned_quality: 31
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 29
next_mate_position {
  reference_name: "20"
  position: 38091807
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:2102:12049:64927"
proper_placement: true
fragment_length: 763
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091592
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 112
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 8
  }
}
aligned_sequence: "CTCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGA"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
next_mate_position {
  reference_name: "20"
  position: 38092207
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1210:18770:99355"
proper_placement: true
fragment_length: 349
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091593
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 111
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 9
  }
}
aligned_sequence: "TCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGAC"
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 39
aligned_quality: 39
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 38
aligned_quality: 32
aligned_quality: 34
aligned_quality: 33
aligned_quality: 38
aligned_quality: 36
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 30
aligned_quality: 32
aligned_quality: 31
aligned_quality: 16
aligned_quality: 34
aligned_quality: 38
aligned_quality: 36
aligned_quality: 36
aligned_quality: 39
aligned_quality: 31
aligned_quality: 28
aligned_quality: 23
aligned_quality: 30
aligned_quality: 30
aligned_quality: 37
aligned_quality: 33
aligned_quality: 39
aligned_quality: 33
aligned_quality: 35
aligned_quality: 39
aligned_quality: 34
aligned_quality: 24
aligned_quality: 33
aligned_quality: 37
aligned_quality: 29
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 21
aligned_quality: 26
aligned_quality: 37
aligned_quality: 40
aligned_quality: 26
aligned_quality: 31
aligned_quality: 35
aligned_quality: 34
aligned_quality: 26
aligned_quality: 30
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 36
aligned_quality: 31
aligned_quality: 13
aligned_quality: 26
aligned_quality: 32
aligned_quality: 30
aligned_quality: 26
aligned_quality: 34
aligned_quality: 31
aligned_quality: 30
aligned_quality: 29
aligned_quality: 26
aligned_quality: 26
aligned_quality: 20
aligned_quality: 26
aligned_quality: 31
aligned_quality: 29
aligned_quality: 32
aligned_quality: 34
aligned_quality: 32
aligned_quality: 29
aligned_quality: 34
aligned_quality: 25
aligned_quality: 31
aligned_quality: 32
aligned_quality: 34
aligned_quality: 29
aligned_quality: 34
aligned_quality: 31
aligned_quality: 20
aligned_quality: 26
aligned_quality: 31
aligned_quality: 29
aligned_quality: 32
aligned_quality: 34
aligned_quality: 29
aligned_quality: 29
aligned_quality: 32
aligned_quality: 31
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 29
aligned_quality: 31
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 27
aligned_quality: 31
aligned_quality: 27
aligned_quality: 20
aligned_quality: 24
aligned_quality: 23
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 27
aligned_quality: 32
aligned_quality: 30
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 19
aligned_quality: 25
aligned_quality: 25
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 29
aligned_quality: 25
aligned_quality: 29
aligned_quality: 24
aligned_quality: 23
aligned_quality: 30
next_mate_position {
  reference_name: "20"
  position: 38091794
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:2212:11715:88582"
proper_placement: true
fragment_length: 560
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091593
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TCTCAAGATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATT"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 33
aligned_quality: 38
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 31
aligned_quality: 35
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 32
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 25
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38092005
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1207:12572:17776"
proper_placement: true
fragment_length: -284
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091599
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 105
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 15
  }
}
aligned_sequence: "GATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGT"
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
aligned_quality: 32
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 32
aligned_quality: 29
aligned_quality: 34
aligned_quality: 31
aligned_quality: 32
aligned_quality: 30
aligned_quality: 24
aligned_quality: 23
aligned_quality: 31
aligned_quality: 31
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 30
aligned_quality: 27
aligned_quality: 30
aligned_quality: 30
aligned_quality: 27
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 29
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 33
aligned_quality: 36
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 39
aligned_quality: 34
aligned_quality: 36
aligned_quality: 38
aligned_quality: 38
aligned_quality: 35
aligned_quality: 40
aligned_quality: 39
aligned_quality: 36
aligned_quality: 41
aligned_quality: 39
aligned_quality: 36
aligned_quality: 32
aligned_quality: 35
aligned_quality: 34
aligned_quality: 38
aligned_quality: 37
aligned_quality: 33
aligned_quality: 40
aligned_quality: 38
aligned_quality: 37
aligned_quality: 36
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 35
aligned_quality: 40
aligned_quality: 38
aligned_quality: 33
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 32
aligned_quality: 37
aligned_quality: 37
aligned_quality: 38
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 27
aligned_quality: 38
aligned_quality: 32
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 31
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091420
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:2204:7375:3719"
proper_placement: true
fragment_length: 723
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091599
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 105
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 15
  }
}
aligned_sequence: "GATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGT"
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 37
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38092174
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1112:11362:81406"
proper_placement: true
fragment_length: 472
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091600
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "ATTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTC"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 32
aligned_quality: 36
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 32
aligned_quality: 35
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 25
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38091924
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:2201:17379:89343"
proper_placement: true
fragment_length: -402
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091601
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 103
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 17
  }
}
aligned_sequence: "TTAAAAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTT"
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 38
aligned_quality: 37
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091302
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:2106:13016:59870"
proper_placement: true
fragment_length: 427
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091605
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AAATCAAGGGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCT"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091884
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1211:12440:66079"
proper_placement: true
fragment_length: -558
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091613
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 91
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 29
  }
}
aligned_sequence: "GGAAAAAGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGC"
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 35
aligned_quality: 37
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 36
aligned_quality: 35
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 34
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 40
aligned_quality: 37
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091146
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:2207:16783:11502"
proper_placement: true
fragment_length: -708
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091619
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 85
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 35
  }
}
aligned_sequence: "AGTGCCCAGGGCCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTAT"
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 34
aligned_quality: 38
aligned_quality: 34
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 35
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 36
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 36
aligned_quality: 37
aligned_quality: 33
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38090996
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:2111:17375:48209"
proper_placement: true
fragment_length: 490
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091623
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "CCCAGGGCCAAATATTTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGA"
aligned_quality: 31
aligned_quality: 34
aligned_quality: 31
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 38
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 11
aligned_quality: 17
aligned_quality: 32
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 8
aligned_quality: 30
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 29
aligned_quality: 29
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091965
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:2213:12633:88467"
proper_placement: true
fragment_length: 523
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091630
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 74
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 46
  }
}
aligned_sequence: "CCAAATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGC"
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 36
aligned_quality: 33
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 34
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 36
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 29
aligned_quality: 32
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38092005
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:2202:16956:73480"
proper_placement: true
fragment_length: 680
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091634
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "ATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACAC"
aligned_quality: 30
aligned_quality: 31
aligned_quality: 31
aligned_quality: 35
aligned_quality: 37
aligned_quality: 33
aligned_quality: 35
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 36
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 38
aligned_quality: 36
aligned_quality: 40
aligned_quality: 34
aligned_quality: 38
aligned_quality: 34
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 33
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 25
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38092166
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:2209:14983:63393"
proper_placement: true
fragment_length: -720
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091634
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 70
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 50
  }
}
aligned_sequence: "ATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAG"
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 33
aligned_quality: 30
aligned_quality: 27
aligned_quality: 32
aligned_quality: 31
aligned_quality: 35
aligned_quality: 33
aligned_quality: 30
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 33
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 28
aligned_quality: 26
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 33
aligned_quality: 36
aligned_quality: 33
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 33
aligned_quality: 37
aligned_quality: 36
aligned_quality: 34
aligned_quality: 37
aligned_quality: 36
aligned_quality: 32
aligned_quality: 26
aligned_quality: 39
aligned_quality: 34
aligned_quality: 28
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 34
aligned_quality: 36
aligned_quality: 38
aligned_quality: 34
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 35
aligned_quality: 37
aligned_quality: 40
aligned_quality: 38
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 33
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 37
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 37
aligned_quality: 34
aligned_quality: 31
aligned_quality: 39
aligned_quality: 36
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 37
aligned_quality: 35
aligned_quality: 37
aligned_quality: 39
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 26
aligned_quality: 28
aligned_quality: 33
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091039
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:2101:4179:86947"
proper_placement: true
fragment_length: 755
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091634
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "ATATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACAC"
aligned_quality: 33
aligned_quality: 31
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 32
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38092241
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:2216:19012:73322"
proper_placement: true
fragment_length: 609
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091635
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 69
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 51
  }
}
aligned_sequence: "TATGTTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGC"
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 32
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 37
aligned_quality: 34
aligned_quality: 38
aligned_quality: 33
aligned_quality: 38
aligned_quality: 39
aligned_quality: 27
aligned_quality: 35
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 35
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 29
aligned_quality: 32
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 27
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 31
aligned_quality: 33
aligned_quality: 19
aligned_quality: 29
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 25
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 29
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38092096
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:1101:2135:25803"
proper_placement: true
fragment_length: 589
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091639
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 65
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 55
  }
}
aligned_sequence: "TTTTGGGTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACC"
aligned_quality: 27
aligned_quality: 31
aligned_quality: 31
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 37
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 34
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 37
aligned_quality: 39
aligned_quality: 36
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 36
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 37
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 37
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 36
aligned_quality: 37
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 35
aligned_quality: 30
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 33
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 30
aligned_quality: 23
aligned_quality: 27
aligned_quality: 32
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 31
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 24
aligned_quality: 29
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 27
next_mate_position {
  reference_name: "20"
  position: 38092080
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1213:13672:97549"
proper_placement: true
fragment_length: 488
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091645
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "GTTTTGCAGGACAAAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAA"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 31
aligned_quality: 35
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38092023
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:1206:5375:45487"
proper_placement: true
fragment_length: -693
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091658
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AAGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAG"
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 27
aligned_quality: 24
aligned_quality: 31
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 37
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 34
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 36
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 36
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 38
aligned_quality: 37
aligned_quality: 30
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
next_mate_position {
  reference_name: "20"
  position: 38091113
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:1112:14729:98076"
proper_placement: true
fragment_length: -478
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091659
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGT"
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 35
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 37
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
next_mate_position {
  reference_name: "20"
  position: 38091329
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1203:5034:24189"
proper_placement: true
fragment_length: -459
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091659
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 45
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 75
  }
}
aligned_sequence: "AGTATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAA"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 25
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 31
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 24
aligned_quality: 24
aligned_quality: 31
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 29
aligned_quality: 19
aligned_quality: 32
aligned_quality: 30
aligned_quality: 33
aligned_quality: 33
aligned_quality: 30
aligned_quality: 33
aligned_quality: 33
aligned_quality: 27
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 32
aligned_quality: 26
aligned_quality: 24
aligned_quality: 33
aligned_quality: 27
aligned_quality: 24
aligned_quality: 27
aligned_quality: 32
aligned_quality: 32
aligned_quality: 33
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 39
aligned_quality: 32
aligned_quality: 39
aligned_quality: 36
aligned_quality: 35
aligned_quality: 38
aligned_quality: 36
aligned_quality: 36
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 37
aligned_quality: 34
aligned_quality: 36
aligned_quality: 39
aligned_quality: 34
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 36
aligned_quality: 36
aligned_quality: 40
aligned_quality: 38
aligned_quality: 37
aligned_quality: 37
aligned_quality: 33
aligned_quality: 24
aligned_quality: 37
aligned_quality: 26
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 33
aligned_quality: 38
aligned_quality: 39
aligned_quality: 36
aligned_quality: 34
aligned_quality: 32
aligned_quality: 37
aligned_quality: 25
aligned_quality: 38
aligned_quality: 36
aligned_quality: 37
aligned_quality: 31
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091325
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:1210:2227:87318"
proper_placement: true
fragment_length: -531
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091661
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 43
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 77
  }
}
aligned_sequence: "TATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACC"
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 32
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 35
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 39
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 37
aligned_quality: 35
aligned_quality: 31
aligned_quality: 31
aligned_quality: 33
next_mate_position {
  reference_name: "20"
  position: 38091255
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1205:15334:50380"
proper_placement: true
fragment_length: 535
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091661
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 43
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 77
  }
}
aligned_sequence: "TATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACC"
aligned_quality: 31
aligned_quality: 30
aligned_quality: 31
aligned_quality: 30
aligned_quality: 35
aligned_quality: 28
aligned_quality: 32
aligned_quality: 33
aligned_quality: 37
aligned_quality: 39
aligned_quality: 32
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 36
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 36
aligned_quality: 39
aligned_quality: 29
aligned_quality: 39
aligned_quality: 36
aligned_quality: 39
aligned_quality: 29
aligned_quality: 30
aligned_quality: 37
aligned_quality: 32
aligned_quality: 34
aligned_quality: 38
aligned_quality: 24
aligned_quality: 37
aligned_quality: 30
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 34
aligned_quality: 36
aligned_quality: 38
aligned_quality: 31
aligned_quality: 37
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 34
aligned_quality: 38
aligned_quality: 38
aligned_quality: 33
aligned_quality: 33
aligned_quality: 36
aligned_quality: 31
aligned_quality: 35
aligned_quality: 38
aligned_quality: 36
aligned_quality: 34
aligned_quality: 36
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 31
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 36
aligned_quality: 34
aligned_quality: 33
aligned_quality: 31
aligned_quality: 33
aligned_quality: 32
aligned_quality: 33
aligned_quality: 32
aligned_quality: 33
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 29
aligned_quality: 34
aligned_quality: 33
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 29
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 31
aligned_quality: 34
aligned_quality: 33
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 33
aligned_quality: 23
aligned_quality: 30
aligned_quality: 31
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 27
aligned_quality: 27
aligned_quality: 32
aligned_quality: 30
aligned_quality: 27
aligned_quality: 27
aligned_quality: 31
aligned_quality: 31
aligned_quality: 23
next_mate_position {
  reference_name: "20"
  position: 38092048
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:2107:9338:59910"
proper_placement: true
fragment_length: 746
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091662
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "ATGGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAA"
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 35
aligned_quality: 32
aligned_quality: 39
aligned_quality: 35
aligned_quality: 35
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 29
aligned_quality: 37
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 30
aligned_quality: 35
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 36
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 37
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 13
aligned_quality: 31
aligned_quality: 28
aligned_quality: 34
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 38
aligned_quality: 37
aligned_quality: 36
aligned_quality: 29
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 29
aligned_quality: 31
aligned_quality: 29
aligned_quality: 32
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 35
aligned_quality: 32
aligned_quality: 30
aligned_quality: 30
aligned_quality: 27
aligned_quality: 30
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 33
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 32
aligned_quality: 30
aligned_quality: 32
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 31
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 30
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
next_mate_position {
  reference_name: "20"
  position: 38092260
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1209:9087:43494"
proper_placement: true
fragment_length: -512
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091664
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 40
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 80
  }
}
aligned_sequence: "GGTTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATA"
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 37
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091277
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:2203:6514:39453"
proper_placement: true
fragment_length: -624
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091666
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TTGAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATG"
aligned_quality: 31
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 35
aligned_quality: 40
aligned_quality: 38
aligned_quality: 35
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 37
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091190
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:2210:11303:57187"
proper_placement: true
fragment_length: 541
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091668
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "GAAACTGAGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTC"
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 37
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
next_mate_position {
  reference_name: "20"
  position: 38092061
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:1115:13474:43118"
proper_placement: true
fragment_length: 479
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091675
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AGCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAAT"
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 30
aligned_quality: 37
aligned_quality: 30
aligned_quality: 27
aligned_quality: 37
aligned_quality: 35
aligned_quality: 37
aligned_quality: 39
aligned_quality: 18
aligned_quality: 30
aligned_quality: 36
aligned_quality: 39
aligned_quality: 36
aligned_quality: 39
aligned_quality: 40
aligned_quality: 29
aligned_quality: 38
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 27
aligned_quality: 32
aligned_quality: 37
aligned_quality: 38
aligned_quality: 40
aligned_quality: 35
aligned_quality: 32
aligned_quality: 36
aligned_quality: 38
aligned_quality: 39
aligned_quality: 24
aligned_quality: 34
aligned_quality: 36
aligned_quality: 31
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 30
aligned_quality: 35
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 33
aligned_quality: 27
aligned_quality: 30
aligned_quality: 28
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 34
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 29
aligned_quality: 36
aligned_quality: 39
aligned_quality: 40
aligned_quality: 35
aligned_quality: 39
aligned_quality: 34
aligned_quality: 36
aligned_quality: 32
aligned_quality: 26
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 33
aligned_quality: 32
aligned_quality: 29
aligned_quality: 31
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 31
aligned_quality: 27
aligned_quality: 29
aligned_quality: 30
aligned_quality: 31
aligned_quality: 30
aligned_quality: 30
aligned_quality: 31
aligned_quality: 23
aligned_quality: 31
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 31
aligned_quality: 30
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 25
aligned_quality: 29
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 30
aligned_quality: 25
aligned_quality: 25
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 29
aligned_quality: 25
aligned_quality: 19
aligned_quality: 25
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 32
aligned_quality: 25
aligned_quality: 31
aligned_quality: 25
next_mate_position {
  reference_name: "20"
  position: 38092006
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:2112:2924:91840"
proper_placement: true
fragment_length: -769
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091676
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "GCTGAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATT"
aligned_quality: 18
aligned_quality: 32
aligned_quality: 36
aligned_quality: 32
aligned_quality: 29
aligned_quality: 25
aligned_quality: 29
aligned_quality: 27
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 25
aligned_quality: 29
aligned_quality: 32
aligned_quality: 29
aligned_quality: 29
aligned_quality: 29
aligned_quality: 29
aligned_quality: 29
aligned_quality: 27
aligned_quality: 32
aligned_quality: 33
aligned_quality: 32
aligned_quality: 32
aligned_quality: 30
aligned_quality: 32
aligned_quality: 29
aligned_quality: 30
aligned_quality: 29
aligned_quality: 30
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 29
aligned_quality: 29
aligned_quality: 25
aligned_quality: 32
aligned_quality: 25
aligned_quality: 29
aligned_quality: 32
aligned_quality: 29
aligned_quality: 32
aligned_quality: 32
aligned_quality: 29
aligned_quality: 29
aligned_quality: 32
aligned_quality: 32
aligned_quality: 29
aligned_quality: 29
aligned_quality: 32
aligned_quality: 32
aligned_quality: 29
aligned_quality: 20
aligned_quality: 29
aligned_quality: 25
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 29
aligned_quality: 18
aligned_quality: 29
aligned_quality: 20
aligned_quality: 29
aligned_quality: 26
aligned_quality: 29
aligned_quality: 32
aligned_quality: 29
aligned_quality: 29
aligned_quality: 26
aligned_quality: 7
aligned_quality: 31
aligned_quality: 31
aligned_quality: 29
aligned_quality: 24
aligned_quality: 7
aligned_quality: 27
aligned_quality: 28
aligned_quality: 32
aligned_quality: 28
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 26
aligned_quality: 26
aligned_quality: 27
aligned_quality: 32
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 32
aligned_quality: 31
aligned_quality: 40
aligned_quality: 40
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 29
aligned_quality: 31
aligned_quality: 33
aligned_quality: 26
aligned_quality: 25
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 9
aligned_quality: 19
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 24
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 25
aligned_quality: 25
aligned_quality: 25
aligned_quality: 33
aligned_quality: 32
aligned_quality: 40
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 36
aligned_quality: 34
aligned_quality: 27
aligned_quality: 24
aligned_quality: 30
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 28
aligned_quality: 10
aligned_quality: 35
aligned_quality: 33
aligned_quality: 25
aligned_quality: 33
aligned_quality: 28
aligned_quality: 27
aligned_quality: 27
aligned_quality: 30
next_mate_position {
  reference_name: "20"
  position: 38091055
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:2107:12296:85055"
proper_placement: true
fragment_length: -599
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091679
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "GAAGATATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGC"
aligned_quality: 25
aligned_quality: 34
aligned_quality: 34
aligned_quality: 29
aligned_quality: 25
aligned_quality: 19
aligned_quality: 19
aligned_quality: 10
aligned_quality: 25
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 30
aligned_quality: 33
aligned_quality: 34
aligned_quality: 31
aligned_quality: 30
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 31
aligned_quality: 30
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 31
aligned_quality: 19
aligned_quality: 11
aligned_quality: 7
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 26
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
aligned_quality: 29
aligned_quality: 29
aligned_quality: 33
aligned_quality: 34
aligned_quality: 31
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 32
aligned_quality: 37
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 36
aligned_quality: 31
aligned_quality: 34
aligned_quality: 31
aligned_quality: 35
aligned_quality: 39
aligned_quality: 38
aligned_quality: 36
aligned_quality: 38
aligned_quality: 33
aligned_quality: 21
aligned_quality: 37
aligned_quality: 30
aligned_quality: 7
aligned_quality: 40
aligned_quality: 37
aligned_quality: 31
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 34
aligned_quality: 37
aligned_quality: 34
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 36
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 31
aligned_quality: 37
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 36
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 36
aligned_quality: 29
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091228
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:1206:15417:61259"
proper_placement: true
fragment_length: -678
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091685
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "ATGAATAGCACCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAG"
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
next_mate_position {
  reference_name: "20"
  position: 38091155
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1103:6322:100130"
proper_placement: true
fragment_length: -432
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091695
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "CCCATACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAA"
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 25
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 37
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 36
aligned_quality: 39
aligned_quality: 36
aligned_quality: 33
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091411
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1210:18488:11523"
proper_placement: true
fragment_length: -586
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091699
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCAGTTATTCTCACTCTGCCGAGCAACCACACAGACCTCCAAACACAACCATAATAACAAACATCAACAGGTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAGCAAAAAA"
aligned_quality: 17
aligned_quality: 7
aligned_quality: 14
aligned_quality: 18
aligned_quality: 10
aligned_quality: 7
aligned_quality: 8
aligned_quality: 10
aligned_quality: 7
aligned_quality: 7
aligned_quality: 10
aligned_quality: 7
aligned_quality: 17
aligned_quality: 10
aligned_quality: 10
aligned_quality: 19
aligned_quality: 7
aligned_quality: 23
aligned_quality: 21
aligned_quality: 10
aligned_quality: 25
aligned_quality: 7
aligned_quality: 19
aligned_quality: 25
aligned_quality: 14
aligned_quality: 21
aligned_quality: 25
aligned_quality: 28
aligned_quality: 28
aligned_quality: 28
aligned_quality: 26
aligned_quality: 21
aligned_quality: 23
aligned_quality: 7
aligned_quality: 26
aligned_quality: 27
aligned_quality: 25
aligned_quality: 17
aligned_quality: 10
aligned_quality: 7
aligned_quality: 7
aligned_quality: 10
aligned_quality: 17
aligned_quality: 19
aligned_quality: 7
aligned_quality: 17
aligned_quality: 21
aligned_quality: 18
aligned_quality: 7
aligned_quality: 7
aligned_quality: 24
aligned_quality: 21
aligned_quality: 5
aligned_quality: 23
aligned_quality: 14
aligned_quality: 22
aligned_quality: 11
aligned_quality: 7
aligned_quality: 7
aligned_quality: 7
aligned_quality: 17
aligned_quality: 7
aligned_quality: 18
aligned_quality: 21
aligned_quality: 18
aligned_quality: 12
aligned_quality: 13
aligned_quality: 18
aligned_quality: 18
aligned_quality: 24
aligned_quality: 15
aligned_quality: 21
aligned_quality: 24
aligned_quality: 13
aligned_quality: 8
aligned_quality: 24
aligned_quality: 30
aligned_quality: 29
aligned_quality: 25
aligned_quality: 25
aligned_quality: 20
aligned_quality: 30
aligned_quality: 30
aligned_quality: 28
aligned_quality: 14
aligned_quality: 18
aligned_quality: 16
aligned_quality: 23
aligned_quality: 26
aligned_quality: 27
aligned_quality: 13
aligned_quality: 18
aligned_quality: 27
aligned_quality: 13
aligned_quality: 13
aligned_quality: 18
aligned_quality: 13
aligned_quality: 26
aligned_quality: 25
aligned_quality: 22
aligned_quality: 8
aligned_quality: 13
aligned_quality: 8
aligned_quality: 24
aligned_quality: 15
aligned_quality: 8
aligned_quality: 8
aligned_quality: 8
aligned_quality: 27
aligned_quality: 30
aligned_quality: 22
aligned_quality: 24
aligned_quality: 22
aligned_quality: 13
aligned_quality: 7
aligned_quality: 25
aligned_quality: 21
aligned_quality: 18
aligned_quality: 13
aligned_quality: 8
aligned_quality: 26
aligned_quality: 28
aligned_quality: 13
aligned_quality: 28
aligned_quality: 13
aligned_quality: 13
aligned_quality: 8
aligned_quality: 23
aligned_quality: 27
aligned_quality: 13
aligned_quality: 13
aligned_quality: 26
aligned_quality: 25
aligned_quality: 8
aligned_quality: 7
aligned_quality: 16
aligned_quality: 29
aligned_quality: 23
aligned_quality: 31
aligned_quality: 8
aligned_quality: 8
aligned_quality: 14
aligned_quality: 23
aligned_quality: 28
aligned_quality: 20
aligned_quality: 26
aligned_quality: 18
aligned_quality: 22
next_mate_position {
  reference_name: "20"
  position: 38091261
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1204:20818:82136"
proper_placement: true
fragment_length: -368
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091700
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "ACTGTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAAC"
aligned_quality: 7
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 29
aligned_quality: 29
aligned_quality: 25
aligned_quality: 32
aligned_quality: 27
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 30
aligned_quality: 31
aligned_quality: 34
aligned_quality: 30
aligned_quality: 35
aligned_quality: 33
aligned_quality: 34
aligned_quality: 33
aligned_quality: 32
aligned_quality: 31
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 31
aligned_quality: 29
aligned_quality: 24
aligned_quality: 33
aligned_quality: 28
aligned_quality: 21
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 34
aligned_quality: 30
aligned_quality: 32
aligned_quality: 23
aligned_quality: 38
aligned_quality: 36
aligned_quality: 31
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 36
aligned_quality: 34
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 35
aligned_quality: 33
aligned_quality: 27
aligned_quality: 38
aligned_quality: 36
aligned_quality: 37
aligned_quality: 38
aligned_quality: 38
aligned_quality: 36
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 36
aligned_quality: 38
aligned_quality: 34
aligned_quality: 38
aligned_quality: 37
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 37
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091480
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1205:7628:7597"
proper_placement: true
fragment_length: -447
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091703
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "GTCTTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATA"
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 31
aligned_quality: 29
aligned_quality: 31
aligned_quality: 29
aligned_quality: 31
aligned_quality: 34
aligned_quality: 31
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 31
aligned_quality: 29
aligned_quality: 29
aligned_quality: 27
aligned_quality: 33
aligned_quality: 29
aligned_quality: 24
aligned_quality: 33
aligned_quality: 31
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 33
aligned_quality: 30
aligned_quality: 30
aligned_quality: 27
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 31
aligned_quality: 29
aligned_quality: 31
aligned_quality: 30
aligned_quality: 28
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 30
aligned_quality: 33
aligned_quality: 28
aligned_quality: 28
aligned_quality: 36
aligned_quality: 32
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 32
aligned_quality: 37
aligned_quality: 33
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 35
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 36
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 33
aligned_quality: 39
aligned_quality: 38
aligned_quality: 33
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 34
aligned_quality: 32
aligned_quality: 37
aligned_quality: 35
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091404
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:2104:11753:98534"
proper_placement: true
fragment_length: 590
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091706
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TTTTTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTT"
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 32
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 35
aligned_quality: 38
aligned_quality: 40
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 36
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 35
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 33
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38092148
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:2114:18669:25759"
proper_placement: true
fragment_length: 497
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091709
  }
  mapping_quality: 60
  cigar {
    operation: INSERT
    operation_length: 1
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 147
  }
}
aligned_sequence: "ATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCA"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38092057
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:1115:5068:66101"
proper_placement: true
fragment_length: 567
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091704
  }
  mapping_quality: 60
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 132
  }
}
aligned_sequence: "CCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAAC"
aligned_quality: 25
aligned_quality: 30
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 37
aligned_quality: 32
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 33
aligned_quality: 37
aligned_quality: 35
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 29
aligned_quality: 37
aligned_quality: 36
aligned_quality: 39
aligned_quality: 34
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 36
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 34
aligned_quality: 35
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 36
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 36
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 32
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 36
aligned_quality: 34
aligned_quality: 34
aligned_quality: 36
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 24
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38092128
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:1101:9814:56688"
proper_placement: true
fragment_length: -413
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091690
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 14
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 106
  }
}
aligned_sequence: "TAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGT"
aligned_quality: 31
aligned_quality: 35
aligned_quality: 31
aligned_quality: 29
aligned_quality: 24
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 32
aligned_quality: 29
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 34
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 37
aligned_quality: 33
aligned_quality: 37
aligned_quality: 33
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 35
aligned_quality: 39
aligned_quality: 35
aligned_quality: 30
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 36
aligned_quality: 37
aligned_quality: 34
aligned_quality: 38
aligned_quality: 37
aligned_quality: 36
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091402
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:2210:2307:87119"
proper_placement: true
fragment_length: -589
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091673
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 31
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 89
  }
}
aligned_sequence: "TGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAAC"
aligned_quality: 18
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 33
aligned_quality: 24
aligned_quality: 24
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 32
aligned_quality: 32
aligned_quality: 29
aligned_quality: 33
aligned_quality: 32
aligned_quality: 33
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 33
aligned_quality: 32
aligned_quality: 30
aligned_quality: 23
aligned_quality: 24
aligned_quality: 30
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 33
aligned_quality: 29
aligned_quality: 36
aligned_quality: 37
aligned_quality: 36
aligned_quality: 39
aligned_quality: 34
aligned_quality: 32
aligned_quality: 22
aligned_quality: 28
aligned_quality: 31
aligned_quality: 34
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 32
aligned_quality: 34
aligned_quality: 22
aligned_quality: 36
aligned_quality: 30
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 36
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 37
aligned_quality: 38
aligned_quality: 36
aligned_quality: 38
aligned_quality: 38
aligned_quality: 36
aligned_quality: 34
aligned_quality: 39
aligned_quality: 37
aligned_quality: 34
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 36
aligned_quality: 39
aligned_quality: 37
aligned_quality: 31
aligned_quality: 33
aligned_quality: 35
aligned_quality: 34
aligned_quality: 33
aligned_quality: 37
aligned_quality: 34
aligned_quality: 11
aligned_quality: 37
aligned_quality: 39
aligned_quality: 35
aligned_quality: 35
aligned_quality: 30
aligned_quality: 37
aligned_quality: 37
aligned_quality: 31
aligned_quality: 30
aligned_quality: 27
next_mate_position {
  reference_name: "20"
  position: 38091209
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1208:19668:2832"
proper_placement: true
fragment_length: -347
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091704
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: INSERT
    operation_length: 15
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 121
  }
}
aligned_sequence: "TGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGT"
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 29
aligned_quality: 33
aligned_quality: 30
aligned_quality: 28
aligned_quality: 14
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091483
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1203:13680:101161"
proper_placement: true
fragment_length: -431
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091704
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: INSERT
    operation_length: 13
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 123
  }
}
aligned_sequence: "CTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTA"
aligned_quality: 27
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091401
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:2113:12430:75730"
proper_placement: true
fragment_length: 495
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091687
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 17
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 103
  }
}
aligned_sequence: "GAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAA"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 32
aligned_quality: 36
aligned_quality: 39
aligned_quality: 36
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 32
next_mate_position {
  reference_name: "20"
  position: 38092056
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1101:1167:6852"
proper_placement: true
fragment_length: 336
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091670
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 34
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 86
  }
}
aligned_sequence: "AACTGAGCTGAAGATATGAATAGCACCCATACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACA"
aligned_quality: 31
aligned_quality: 31
aligned_quality: 33
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 34
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 30
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38091897
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1201:18221:91791"
proper_placement: true
fragment_length: 523
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091704
  }
  mapping_quality: 60
  cigar {
    operation: INSERT
    operation_length: 13
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 123
  }
}
aligned_sequence: "CTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTA"
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38092084
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1211:16612:5911"
proper_placement: true
fragment_length: -638
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091700
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 16
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 116
  }
}
aligned_sequence: "ACTGCTGCTGGGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTT"
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091187
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:2102:1839:96362"
proper_placement: true
fragment_length: 580
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091704
  }
  mapping_quality: 60
  cigar {
    operation: INSERT
    operation_length: 10
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 4
  }
  cigar {
    operation: INSERT
    operation_length: 4
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 1
  }
  cigar {
    operation: INSERT
    operation_length: 3
  }
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 126
  }
}
aligned_sequence: "GGGGACCCACTCCTCAAATCCATTGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCC"
aligned_quality: 31
aligned_quality: 31
aligned_quality: 27
aligned_quality: 35
aligned_quality: 28
aligned_quality: 31
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 36
aligned_quality: 27
aligned_quality: 37
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 36
aligned_quality: 40
aligned_quality: 38
aligned_quality: 33
aligned_quality: 18
aligned_quality: 33
aligned_quality: 36
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 35
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 34
aligned_quality: 34
aligned_quality: 36
aligned_quality: 39
aligned_quality: 29
aligned_quality: 33
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 36
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 36
aligned_quality: 37
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 34
aligned_quality: 29
aligned_quality: 39
aligned_quality: 32
aligned_quality: 38
aligned_quality: 33
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 30
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 24
aligned_quality: 31
aligned_quality: 27
aligned_quality: 30
aligned_quality: 30
aligned_quality: 33
aligned_quality: 30
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 29
aligned_quality: 29
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 25
aligned_quality: 34
aligned_quality: 31
aligned_quality: 31
aligned_quality: 19
aligned_quality: 31
aligned_quality: 32
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 29
aligned_quality: 32
aligned_quality: 32
next_mate_position {
  reference_name: "20"
  position: 38092141
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:1205:9619:40726"
proper_placement: true
fragment_length: -467
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091710
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TGAAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAA"
aligned_quality: 32
aligned_quality: 29
aligned_quality: 29
aligned_quality: 32
aligned_quality: 29
aligned_quality: 25
aligned_quality: 19
aligned_quality: 29
aligned_quality: 29
aligned_quality: 32
aligned_quality: 25
aligned_quality: 29
aligned_quality: 25
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 29
aligned_quality: 25
aligned_quality: 32
aligned_quality: 32
aligned_quality: 25
aligned_quality: 29
aligned_quality: 29
aligned_quality: 25
aligned_quality: 19
aligned_quality: 25
aligned_quality: 32
aligned_quality: 36
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 25
aligned_quality: 29
aligned_quality: 32
aligned_quality: 29
aligned_quality: 29
aligned_quality: 19
aligned_quality: 19
aligned_quality: 27
aligned_quality: 17
aligned_quality: 13
aligned_quality: 29
aligned_quality: 27
aligned_quality: 26
aligned_quality: 27
aligned_quality: 25
aligned_quality: 25
aligned_quality: 27
aligned_quality: 27
aligned_quality: 27
aligned_quality: 18
aligned_quality: 28
aligned_quality: 24
aligned_quality: 20
aligned_quality: 29
aligned_quality: 32
aligned_quality: 24
aligned_quality: 25
aligned_quality: 25
aligned_quality: 17
aligned_quality: 28
aligned_quality: 29
aligned_quality: 26
aligned_quality: 28
aligned_quality: 31
aligned_quality: 26
aligned_quality: 21
aligned_quality: 21
aligned_quality: 11
aligned_quality: 11
aligned_quality: 11
aligned_quality: 13
aligned_quality: 8
aligned_quality: 22
aligned_quality: 26
aligned_quality: 28
aligned_quality: 13
aligned_quality: 35
aligned_quality: 32
aligned_quality: 22
aligned_quality: 32
aligned_quality: 34
aligned_quality: 36
aligned_quality: 34
aligned_quality: 40
aligned_quality: 35
aligned_quality: 31
aligned_quality: 28
aligned_quality: 8
aligned_quality: 32
aligned_quality: 34
aligned_quality: 40
aligned_quality: 35
aligned_quality: 34
aligned_quality: 33
aligned_quality: 27
aligned_quality: 28
aligned_quality: 31
aligned_quality: 33
aligned_quality: 32
aligned_quality: 36
aligned_quality: 35
aligned_quality: 30
aligned_quality: 24
aligned_quality: 30
aligned_quality: 30
aligned_quality: 26
aligned_quality: 27
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 30
aligned_quality: 27
aligned_quality: 25
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 30
aligned_quality: 27
aligned_quality: 27
aligned_quality: 36
aligned_quality: 37
aligned_quality: 24
aligned_quality: 18
aligned_quality: 36
aligned_quality: 37
aligned_quality: 25
aligned_quality: 36
aligned_quality: 36
aligned_quality: 25
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 30
aligned_quality: 35
aligned_quality: 32
aligned_quality: 28
aligned_quality: 26
aligned_quality: 25
aligned_quality: 26
aligned_quality: 35
aligned_quality: 30
aligned_quality: 30
aligned_quality: 28
next_mate_position {
  reference_name: "20"
  position: 38091391
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:2216:13181:53882"
proper_placement: true
fragment_length: -375
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091712
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AAGGACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGT"
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 30
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 28
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 29
aligned_quality: 32
aligned_quality: 24
aligned_quality: 28
aligned_quality: 30
aligned_quality: 33
aligned_quality: 31
aligned_quality: 36
aligned_quality: 39
aligned_quality: 36
aligned_quality: 36
aligned_quality: 32
aligned_quality: 36
aligned_quality: 34
aligned_quality: 36
aligned_quality: 40
aligned_quality: 36
aligned_quality: 39
aligned_quality: 36
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 38
aligned_quality: 32
aligned_quality: 32
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 37
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 35
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 33
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 33
aligned_quality: 37
aligned_quality: 34
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091485
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1112:17631:32592"
proper_placement: true
fragment_length: -326
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091715
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "GACCAGAGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAA"
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 33
aligned_quality: 28
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 35
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091537
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1205:4591:80210"
proper_placement: true
fragment_length: -621
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091721
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AGTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAA"
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091248
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1201:5691:34882"
proper_placement: true
fragment_length: 785
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091722
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "GTTTTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAG"
aligned_quality: 31
aligned_quality: 33
aligned_quality: 31
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38092359
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1212:11493:48428"
proper_placement: true
fragment_length: 616
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091725
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACG"
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 35
aligned_quality: 39
aligned_quality: 36
aligned_quality: 37
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 31
aligned_quality: 34
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 33
aligned_quality: 34
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 34
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 29
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
next_mate_position {
  reference_name: "20"
  position: 38092193
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1208:1530:22543"
proper_placement: true
fragment_length: -679
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091725
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TTATTCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACG"
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 25
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 30
aligned_quality: 24
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 31
aligned_quality: 29
aligned_quality: 25
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 27
aligned_quality: 30
aligned_quality: 33
aligned_quality: 27
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 27
aligned_quality: 27
aligned_quality: 24
aligned_quality: 33
aligned_quality: 34
aligned_quality: 31
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 31
aligned_quality: 36
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 31
aligned_quality: 39
aligned_quality: 39
aligned_quality: 34
aligned_quality: 36
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 36
aligned_quality: 33
aligned_quality: 39
aligned_quality: 37
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 27
aligned_quality: 31
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091194
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:2106:13889:77270"
proper_placement: true
fragment_length: -424
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091729
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TCACAGAGCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTC"
aligned_quality: 25
aligned_quality: 33
aligned_quality: 33
aligned_quality: 29
aligned_quality: 29
aligned_quality: 7
aligned_quality: 29
aligned_quality: 19
aligned_quality: 18
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 31
aligned_quality: 33
aligned_quality: 32
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 30
aligned_quality: 30
aligned_quality: 29
aligned_quality: 24
aligned_quality: 27
aligned_quality: 27
aligned_quality: 27
aligned_quality: 29
aligned_quality: 25
aligned_quality: 7
aligned_quality: 23
aligned_quality: 17
aligned_quality: 18
aligned_quality: 27
aligned_quality: 23
aligned_quality: 32
aligned_quality: 29
aligned_quality: 33
aligned_quality: 27
aligned_quality: 29
aligned_quality: 25
aligned_quality: 15
aligned_quality: 23
aligned_quality: 17
aligned_quality: 10
aligned_quality: 17
aligned_quality: 24
aligned_quality: 30
aligned_quality: 25
aligned_quality: 27
aligned_quality: 20
aligned_quality: 11
aligned_quality: 20
aligned_quality: 25
aligned_quality: 20
aligned_quality: 7
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 33
aligned_quality: 30
aligned_quality: 28
aligned_quality: 18
aligned_quality: 31
aligned_quality: 33
aligned_quality: 31
aligned_quality: 26
aligned_quality: 29
aligned_quality: 26
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
aligned_quality: 25
aligned_quality: 31
aligned_quality: 25
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 31
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 28
aligned_quality: 36
aligned_quality: 35
aligned_quality: 31
aligned_quality: 28
aligned_quality: 8
aligned_quality: 25
aligned_quality: 37
aligned_quality: 31
aligned_quality: 26
aligned_quality: 31
aligned_quality: 36
aligned_quality: 33
aligned_quality: 37
aligned_quality: 37
aligned_quality: 33
aligned_quality: 30
aligned_quality: 37
aligned_quality: 35
aligned_quality: 29
aligned_quality: 31
aligned_quality: 27
aligned_quality: 40
aligned_quality: 37
aligned_quality: 37
aligned_quality: 40
aligned_quality: 38
aligned_quality: 36
aligned_quality: 37
aligned_quality: 34
aligned_quality: 30
aligned_quality: 37
aligned_quality: 37
aligned_quality: 32
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 32
aligned_quality: 19
aligned_quality: 37
aligned_quality: 34
aligned_quality: 31
aligned_quality: 33
aligned_quality: 39
aligned_quality: 37
aligned_quality: 38
aligned_quality: 32
aligned_quality: 27
aligned_quality: 35
aligned_quality: 30
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 29
aligned_quality: 35
aligned_quality: 28
aligned_quality: 35
aligned_quality: 35
aligned_quality: 30
aligned_quality: 31
aligned_quality: 30
aligned_quality: 26
next_mate_position {
  reference_name: "20"
  position: 38091453
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1107:10030:53790"
proper_placement: true
fragment_length: -308
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091736
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "GCATTTATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAA"
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 31
aligned_quality: 25
aligned_quality: 32
aligned_quality: 29
aligned_quality: 19
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 27
aligned_quality: 29
aligned_quality: 31
aligned_quality: 29
aligned_quality: 34
aligned_quality: 29
aligned_quality: 25
aligned_quality: 8
aligned_quality: 31
aligned_quality: 31
aligned_quality: 27
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 30
aligned_quality: 24
aligned_quality: 30
aligned_quality: 29
aligned_quality: 27
aligned_quality: 25
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 31
aligned_quality: 29
aligned_quality: 34
aligned_quality: 24
aligned_quality: 27
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 32
aligned_quality: 30
aligned_quality: 35
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 34
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 37
aligned_quality: 31
aligned_quality: 35
aligned_quality: 30
aligned_quality: 23
aligned_quality: 35
aligned_quality: 35
aligned_quality: 38
aligned_quality: 38
aligned_quality: 36
aligned_quality: 38
aligned_quality: 38
aligned_quality: 34
aligned_quality: 36
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 33
aligned_quality: 29
aligned_quality: 38
aligned_quality: 38
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 19
aligned_quality: 38
aligned_quality: 37
aligned_quality: 33
aligned_quality: 36
aligned_quality: 40
aligned_quality: 38
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 30
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 23
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091576
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:1207:15086:27783"
proper_placement: true
fragment_length: 558
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091741
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TATTCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTC"
aligned_quality: 10
aligned_quality: 28
aligned_quality: 10
aligned_quality: 30
aligned_quality: 30
aligned_quality: 23
aligned_quality: 11
aligned_quality: 27
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 32
aligned_quality: 18
aligned_quality: 22
aligned_quality: 32
aligned_quality: 15
aligned_quality: 32
aligned_quality: 28
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 32
aligned_quality: 32
aligned_quality: 33
aligned_quality: 32
aligned_quality: 32
aligned_quality: 29
aligned_quality: 29
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 28
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 27
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 29
aligned_quality: 18
aligned_quality: 29
aligned_quality: 33
aligned_quality: 28
aligned_quality: 29
aligned_quality: 32
aligned_quality: 29
aligned_quality: 29
aligned_quality: 31
aligned_quality: 18
aligned_quality: 29
aligned_quality: 29
aligned_quality: 26
aligned_quality: 29
aligned_quality: 29
aligned_quality: 31
aligned_quality: 31
aligned_quality: 29
aligned_quality: 26
aligned_quality: 20
aligned_quality: 21
aligned_quality: 20
aligned_quality: 26
aligned_quality: 29
aligned_quality: 26
aligned_quality: 26
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 28
aligned_quality: 24
aligned_quality: 20
aligned_quality: 20
aligned_quality: 24
aligned_quality: 24
aligned_quality: 11
aligned_quality: 24
aligned_quality: 24
aligned_quality: 26
aligned_quality: 26
aligned_quality: 28
aligned_quality: 30
aligned_quality: 28
aligned_quality: 28
aligned_quality: 28
aligned_quality: 27
aligned_quality: 26
aligned_quality: 28
aligned_quality: 26
aligned_quality: 26
aligned_quality: 26
aligned_quality: 26
aligned_quality: 26
aligned_quality: 26
aligned_quality: 26
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 28
aligned_quality: 28
aligned_quality: 25
aligned_quality: 26
aligned_quality: 30
aligned_quality: 26
aligned_quality: 23
aligned_quality: 25
aligned_quality: 28
aligned_quality: 25
aligned_quality: 25
aligned_quality: 30
aligned_quality: 26
aligned_quality: 28
aligned_quality: 28
aligned_quality: 30
aligned_quality: 28
aligned_quality: 30
aligned_quality: 30
aligned_quality: 27
aligned_quality: 24
aligned_quality: 23
aligned_quality: 26
aligned_quality: 27
aligned_quality: 27
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 23
aligned_quality: 26
aligned_quality: 18
next_mate_position {
  reference_name: "20"
  position: 38092151
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1208:16019:91136"
proper_placement: true
fragment_length: -576
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091744
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TCTCACTCTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCC"
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 35
aligned_quality: 33
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 33
aligned_quality: 31
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091316
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:2214:13506:9222"
proper_placement: true
fragment_length: 831
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091748
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "ACTCTGCCGATCAGCCACACATACCACGAAACACAACCATAATAACAGACATCAACAGTTAAAAGGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAAGATACTTCTAAGGCAAACTAAAGACGTCTCTTTCTAAATTTCCCCTGCA"
aligned_quality: 7
aligned_quality: 7
aligned_quality: 7
aligned_quality: 17
aligned_quality: 27
aligned_quality: 31
aligned_quality: 31
aligned_quality: 27
aligned_quality: 18
aligned_quality: 27
aligned_quality: 7
aligned_quality: 7
aligned_quality: 15
aligned_quality: 8
aligned_quality: 8
aligned_quality: 13
aligned_quality: 17
aligned_quality: 8
aligned_quality: 23
aligned_quality: 7
aligned_quality: 25
aligned_quality: 8
aligned_quality: 8
aligned_quality: 8
aligned_quality: 16
aligned_quality: 23
aligned_quality: 8
aligned_quality: 16
aligned_quality: 25
aligned_quality: 26
aligned_quality: 18
aligned_quality: 12
aligned_quality: 13
aligned_quality: 18
aligned_quality: 28
aligned_quality: 28
aligned_quality: 20
aligned_quality: 20
aligned_quality: 28
aligned_quality: 26
aligned_quality: 26
aligned_quality: 29
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 23
aligned_quality: 26
aligned_quality: 8
aligned_quality: 13
aligned_quality: 7
aligned_quality: 28
aligned_quality: 30
aligned_quality: 8
aligned_quality: 27
aligned_quality: 28
aligned_quality: 8
aligned_quality: 23
aligned_quality: 29
aligned_quality: 24
aligned_quality: 29
aligned_quality: 18
aligned_quality: 8
aligned_quality: 25
aligned_quality: 24
aligned_quality: 8
aligned_quality: 7
aligned_quality: 8
aligned_quality: 13
aligned_quality: 7
aligned_quality: 13
aligned_quality: 24
aligned_quality: 18
aligned_quality: 28
aligned_quality: 16
aligned_quality: 7
aligned_quality: 12
aligned_quality: 7
aligned_quality: 7
aligned_quality: 12
aligned_quality: 25
aligned_quality: 26
aligned_quality: 12
aligned_quality: 17
aligned_quality: 20
aligned_quality: 20
aligned_quality: 25
aligned_quality: 20
aligned_quality: 7
aligned_quality: 11
aligned_quality: 17
aligned_quality: 7
aligned_quality: 7
aligned_quality: 8
aligned_quality: 23
aligned_quality: 17
aligned_quality: 25
aligned_quality: 25
aligned_quality: 7
aligned_quality: 5
aligned_quality: 5
aligned_quality: 10
aligned_quality: 10
aligned_quality: 10
aligned_quality: 17
aligned_quality: 25
aligned_quality: 19
aligned_quality: 25
aligned_quality: 7
aligned_quality: 7
aligned_quality: 7
aligned_quality: 7
aligned_quality: 7
aligned_quality: 10
aligned_quality: 17
aligned_quality: 10
aligned_quality: 17
aligned_quality: 17
aligned_quality: 18
aligned_quality: 25
aligned_quality: 20
aligned_quality: 21
aligned_quality: 14
aligned_quality: 21
aligned_quality: 22
aligned_quality: 5
aligned_quality: 10
aligned_quality: 5
aligned_quality: 7
aligned_quality: 20
aligned_quality: 21
aligned_quality: 25
aligned_quality: 25
aligned_quality: 20
aligned_quality: 7
aligned_quality: 19
aligned_quality: 14
aligned_quality: 25
aligned_quality: 7
aligned_quality: 18
aligned_quality: 25
aligned_quality: 7
aligned_quality: 7
aligned_quality: 7
aligned_quality: 10
aligned_quality: 7
aligned_quality: 10
aligned_quality: 7
aligned_quality: 7
next_mate_position {
  reference_name: "20"
  position: 38092431
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1206:7920:65998"
proper_placement: true
fragment_length: 592
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091751
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "CTGCCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGA"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 30
next_mate_position {
  reference_name: "20"
  position: 38092195
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1113:2055:59097"
proper_placement: true
fragment_length: 827
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091754
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "CCGAGCAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCT"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 36
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38092433
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1108:14571:30699"
proper_placement: true
fragment_length: 659
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091759
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "CAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAA"
aligned_quality: 27
aligned_quality: 31
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 26
aligned_quality: 35
aligned_quality: 30
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 36
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 30
aligned_quality: 38
aligned_quality: 38
aligned_quality: 32
aligned_quality: 27
aligned_quality: 37
aligned_quality: 38
aligned_quality: 34
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 36
aligned_quality: 34
aligned_quality: 39
aligned_quality: 28
aligned_quality: 31
aligned_quality: 31
aligned_quality: 32
aligned_quality: 31
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 29
aligned_quality: 32
aligned_quality: 36
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 27
aligned_quality: 32
aligned_quality: 33
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 32
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 25
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 29
aligned_quality: 31
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 36
aligned_quality: 29
aligned_quality: 31
aligned_quality: 32
aligned_quality: 29
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 30
aligned_quality: 30
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 25
next_mate_position {
  reference_name: "20"
  position: 38092270
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1203:1303:16206"
proper_placement: true
fragment_length: 492
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091759
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "CAACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAA"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38092103
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:2114:4783:56735"
proper_placement: true
fragment_length: 629
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091760
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAAT"
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 30
aligned_quality: 36
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 32
aligned_quality: 34
aligned_quality: 25
aligned_quality: 29
aligned_quality: 32
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38092241
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:2207:10041:55773"
proper_placement: true
fragment_length: 791
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091760
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAAT"
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 39
aligned_quality: 37
aligned_quality: 32
aligned_quality: 37
aligned_quality: 39
aligned_quality: 35
aligned_quality: 38
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 36
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 35
aligned_quality: 34
aligned_quality: 37
aligned_quality: 39
aligned_quality: 37
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 31
aligned_quality: 33
aligned_quality: 37
aligned_quality: 39
aligned_quality: 38
aligned_quality: 35
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 36
aligned_quality: 29
aligned_quality: 36
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 30
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 19
aligned_quality: 29
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 30
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 25
next_mate_position {
  reference_name: "20"
  position: 38092403
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:2107:4042:52716"
proper_placement: true
fragment_length: 578
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091761
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "ACCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATG"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38092191
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1209:6822:56558"
proper_placement: true
fragment_length: -894
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091762
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "CCACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGA"
aligned_quality: 22
aligned_quality: 33
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 29
aligned_quality: 27
aligned_quality: 27
aligned_quality: 17
aligned_quality: 31
aligned_quality: 29
aligned_quality: 27
aligned_quality: 24
aligned_quality: 10
aligned_quality: 30
aligned_quality: 24
aligned_quality: 33
aligned_quality: 31
aligned_quality: 25
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 29
aligned_quality: 31
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 29
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 36
aligned_quality: 37
aligned_quality: 28
aligned_quality: 37
aligned_quality: 32
aligned_quality: 34
aligned_quality: 28
aligned_quality: 40
aligned_quality: 38
aligned_quality: 32
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 31
aligned_quality: 36
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 30
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 37
aligned_quality: 30
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 32
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 37
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 35
aligned_quality: 40
aligned_quality: 37
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 31
aligned_quality: 31
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091016
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1106:13809:91853"
proper_placement: true
fragment_length: -849
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091764
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "ACACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCGCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATT"
aligned_quality: 7
aligned_quality: 33
aligned_quality: 33
aligned_quality: 30
aligned_quality: 29
aligned_quality: 19
aligned_quality: 20
aligned_quality: 17
aligned_quality: 10
aligned_quality: 7
aligned_quality: 15
aligned_quality: 29
aligned_quality: 18
aligned_quality: 18
aligned_quality: 27
aligned_quality: 33
aligned_quality: 27
aligned_quality: 30
aligned_quality: 32
aligned_quality: 32
aligned_quality: 29
aligned_quality: 29
aligned_quality: 29
aligned_quality: 19
aligned_quality: 32
aligned_quality: 25
aligned_quality: 19
aligned_quality: 7
aligned_quality: 30
aligned_quality: 29
aligned_quality: 32
aligned_quality: 32
aligned_quality: 25
aligned_quality: 19
aligned_quality: 19
aligned_quality: 7
aligned_quality: 19
aligned_quality: 7
aligned_quality: 7
aligned_quality: 29
aligned_quality: 31
aligned_quality: 25
aligned_quality: 19
aligned_quality: 25
aligned_quality: 32
aligned_quality: 31
aligned_quality: 25
aligned_quality: 25
aligned_quality: 23
aligned_quality: 27
aligned_quality: 24
aligned_quality: 33
aligned_quality: 32
aligned_quality: 24
aligned_quality: 32
aligned_quality: 29
aligned_quality: 32
aligned_quality: 32
aligned_quality: 31
aligned_quality: 19
aligned_quality: 7
aligned_quality: 7
aligned_quality: 18
aligned_quality: 29
aligned_quality: 29
aligned_quality: 31
aligned_quality: 25
aligned_quality: 20
aligned_quality: 18
aligned_quality: 17
aligned_quality: 14
aligned_quality: 30
aligned_quality: 28
aligned_quality: 18
aligned_quality: 26
aligned_quality: 20
aligned_quality: 18
aligned_quality: 6
aligned_quality: 28
aligned_quality: 31
aligned_quality: 30
aligned_quality: 28
aligned_quality: 30
aligned_quality: 30
aligned_quality: 26
aligned_quality: 36
aligned_quality: 32
aligned_quality: 30
aligned_quality: 22
aligned_quality: 13
aligned_quality: 35
aligned_quality: 31
aligned_quality: 13
aligned_quality: 8
aligned_quality: 31
aligned_quality: 26
aligned_quality: 28
aligned_quality: 31
aligned_quality: 26
aligned_quality: 28
aligned_quality: 31
aligned_quality: 19
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 31
aligned_quality: 22
aligned_quality: 30
aligned_quality: 15
aligned_quality: 25
aligned_quality: 30
aligned_quality: 9
aligned_quality: 35
aligned_quality: 34
aligned_quality: 24
aligned_quality: 38
aligned_quality: 36
aligned_quality: 35
aligned_quality: 31
aligned_quality: 37
aligned_quality: 36
aligned_quality: 34
aligned_quality: 37
aligned_quality: 32
aligned_quality: 25
aligned_quality: 25
aligned_quality: 36
aligned_quality: 24
aligned_quality: 37
aligned_quality: 38
aligned_quality: 33
aligned_quality: 29
aligned_quality: 34
aligned_quality: 26
aligned_quality: 25
aligned_quality: 19
aligned_quality: 37
aligned_quality: 27
aligned_quality: 35
aligned_quality: 30
aligned_quality: 32
aligned_quality: 26
aligned_quality: 35
aligned_quality: 32
aligned_quality: 16
aligned_quality: 27
aligned_quality: 31
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091063
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:1111:15948:54840"
proper_placement: true
fragment_length: 538
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091766
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "ACAGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAA"
aligned_quality: 31
aligned_quality: 34
aligned_quality: 31
aligned_quality: 35
aligned_quality: 37
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 33
aligned_quality: 35
aligned_quality: 39
aligned_quality: 40
aligned_quality: 36
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 36
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 32
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 33
aligned_quality: 37
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 36
aligned_quality: 39
aligned_quality: 28
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 36
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 35
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38092156
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1115:12510:86015"
proper_placement: true
fragment_length: 705
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091768
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAA"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38092325
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:2211:18638:85306"
proper_placement: true
fragment_length: -669
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091768
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AGACCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAA"
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 31
aligned_quality: 35
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 35
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 34
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091247
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1212:2189:90731"
proper_placement: true
fragment_length: -598
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091771
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "CCACCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCATGCAGGATCTGAAAATGATTAAAAAAG"
aligned_quality: 7
aligned_quality: 27
aligned_quality: 17
aligned_quality: 27
aligned_quality: 29
aligned_quality: 30
aligned_quality: 32
aligned_quality: 30
aligned_quality: 27
aligned_quality: 20
aligned_quality: 17
aligned_quality: 10
aligned_quality: 10
aligned_quality: 7
aligned_quality: 29
aligned_quality: 25
aligned_quality: 35
aligned_quality: 32
aligned_quality: 35
aligned_quality: 32
aligned_quality: 27
aligned_quality: 27
aligned_quality: 18
aligned_quality: 29
aligned_quality: 25
aligned_quality: 19
aligned_quality: 29
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 32
aligned_quality: 29
aligned_quality: 29
aligned_quality: 25
aligned_quality: 32
aligned_quality: 19
aligned_quality: 29
aligned_quality: 29
aligned_quality: 19
aligned_quality: 7
aligned_quality: 17
aligned_quality: 23
aligned_quality: 27
aligned_quality: 29
aligned_quality: 25
aligned_quality: 7
aligned_quality: 29
aligned_quality: 32
aligned_quality: 25
aligned_quality: 32
aligned_quality: 25
aligned_quality: 18
aligned_quality: 32
aligned_quality: 29
aligned_quality: 29
aligned_quality: 18
aligned_quality: 25
aligned_quality: 11
aligned_quality: 11
aligned_quality: 20
aligned_quality: 20
aligned_quality: 14
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 29
aligned_quality: 26
aligned_quality: 26
aligned_quality: 18
aligned_quality: 31
aligned_quality: 31
aligned_quality: 32
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 30
aligned_quality: 22
aligned_quality: 13
aligned_quality: 28
aligned_quality: 13
aligned_quality: 8
aligned_quality: 26
aligned_quality: 32
aligned_quality: 32
aligned_quality: 28
aligned_quality: 22
aligned_quality: 26
aligned_quality: 26
aligned_quality: 28
aligned_quality: 29
aligned_quality: 23
aligned_quality: 8
aligned_quality: 23
aligned_quality: 28
aligned_quality: 32
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 30
aligned_quality: 30
aligned_quality: 18
aligned_quality: 30
aligned_quality: 30
aligned_quality: 19
aligned_quality: 35
aligned_quality: 33
aligned_quality: 24
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 34
aligned_quality: 24
aligned_quality: 23
aligned_quality: 30
aligned_quality: 36
aligned_quality: 37
aligned_quality: 36
aligned_quality: 29
aligned_quality: 34
aligned_quality: 17
aligned_quality: 25
aligned_quality: 37
aligned_quality: 34
aligned_quality: 25
aligned_quality: 19
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 32
aligned_quality: 36
aligned_quality: 35
aligned_quality: 27
aligned_quality: 29
aligned_quality: 35
aligned_quality: 32
aligned_quality: 28
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
next_mate_position {
  reference_name: "20"
  position: 38091321
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1115:7781:61049"
proper_placement: true
fragment_length: -683
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091774
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "CCAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAACCATACTTCAAAGACAAACTAAAGACGACTCTTTCTAAAGTTCCCCTACAGGATCTGAAAATGATTAAAAAAGAAA"
aligned_quality: 7
aligned_quality: 18
aligned_quality: 26
aligned_quality: 23
aligned_quality: 10
aligned_quality: 26
aligned_quality: 20
aligned_quality: 23
aligned_quality: 10
aligned_quality: 10
aligned_quality: 23
aligned_quality: 26
aligned_quality: 30
aligned_quality: 25
aligned_quality: 10
aligned_quality: 23
aligned_quality: 30
aligned_quality: 23
aligned_quality: 23
aligned_quality: 23
aligned_quality: 26
aligned_quality: 23
aligned_quality: 10
aligned_quality: 10
aligned_quality: 7
aligned_quality: 7
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 25
aligned_quality: 18
aligned_quality: 25
aligned_quality: 30
aligned_quality: 25
aligned_quality: 18
aligned_quality: 25
aligned_quality: 23
aligned_quality: 10
aligned_quality: 10
aligned_quality: 23
aligned_quality: 18
aligned_quality: 23
aligned_quality: 23
aligned_quality: 7
aligned_quality: 18
aligned_quality: 23
aligned_quality: 23
aligned_quality: 10
aligned_quality: 7
aligned_quality: 7
aligned_quality: 7
aligned_quality: 25
aligned_quality: 28
aligned_quality: 24
aligned_quality: 28
aligned_quality: 25
aligned_quality: 7
aligned_quality: 20
aligned_quality: 6
aligned_quality: 6
aligned_quality: 26
aligned_quality: 20
aligned_quality: 26
aligned_quality: 11
aligned_quality: 13
aligned_quality: 18
aligned_quality: 31
aligned_quality: 27
aligned_quality: 31
aligned_quality: 29
aligned_quality: 26
aligned_quality: 8
aligned_quality: 30
aligned_quality: 32
aligned_quality: 26
aligned_quality: 28
aligned_quality: 13
aligned_quality: 8
aligned_quality: 8
aligned_quality: 22
aligned_quality: 13
aligned_quality: 24
aligned_quality: 29
aligned_quality: 22
aligned_quality: 13
aligned_quality: 22
aligned_quality: 28
aligned_quality: 28
aligned_quality: 28
aligned_quality: 23
aligned_quality: 8
aligned_quality: 22
aligned_quality: 28
aligned_quality: 24
aligned_quality: 7
aligned_quality: 22
aligned_quality: 7
aligned_quality: 9
aligned_quality: 9
aligned_quality: 8
aligned_quality: 9
aligned_quality: 15
aligned_quality: 9
aligned_quality: 9
aligned_quality: 9
aligned_quality: 9
aligned_quality: 32
aligned_quality: 33
aligned_quality: 30
aligned_quality: 28
aligned_quality: 20
aligned_quality: 25
aligned_quality: 15
aligned_quality: 32
aligned_quality: 30
aligned_quality: 16
aligned_quality: 16
aligned_quality: 9
aligned_quality: 17
aligned_quality: 27
aligned_quality: 17
aligned_quality: 10
aligned_quality: 17
aligned_quality: 27
aligned_quality: 11
aligned_quality: 27
aligned_quality: 11
aligned_quality: 19
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 22
aligned_quality: 17
aligned_quality: 11
aligned_quality: 27
aligned_quality: 28
aligned_quality: 32
aligned_quality: 22
aligned_quality: 29
aligned_quality: 27
aligned_quality: 22
aligned_quality: 32
aligned_quality: 27
aligned_quality: 16
aligned_quality: 29
aligned_quality: 22
aligned_quality: 16
next_mate_position {
  reference_name: "20"
  position: 38091239
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1208:10341:30973"
proper_placement: true
fragment_length: -781
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091775
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "CAAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAAAAGAAAT"
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 33
aligned_quality: 31
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 36
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091142
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:2209:9975:69037"
proper_placement: true
fragment_length: 461
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091776
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AAACACAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAAAAGAAATT"
aligned_quality: 31
aligned_quality: 31
aligned_quality: 27
aligned_quality: 35
aligned_quality: 30
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 35
aligned_quality: 39
aligned_quality: 39
aligned_quality: 33
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 33
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 29
aligned_quality: 33
aligned_quality: 38
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 38
aligned_quality: 35
aligned_quality: 31
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 34
aligned_quality: 27
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 37
aligned_quality: 39
aligned_quality: 31
aligned_quality: 33
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 32
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 37
aligned_quality: 33
aligned_quality: 36
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 34
aligned_quality: 36
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 26
aligned_quality: 32
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 25
aligned_quality: 31
aligned_quality: 27
aligned_quality: 33
aligned_quality: 31
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 29
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 7
aligned_quality: 29
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 30
aligned_quality: 34
aligned_quality: 34
aligned_quality: 27
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 31
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 31
aligned_quality: 32
aligned_quality: 34
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 31
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38092089
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:2212:1077:32349"
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091781
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "CAACCATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATNTGAAANTGANTANAAAAGNAATTATGTC"
aligned_quality: 34
aligned_quality: 33
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 34
aligned_quality: 39
aligned_quality: 36
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 35
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 2
aligned_quality: 10
aligned_quality: 23
aligned_quality: 27
aligned_quality: 31
aligned_quality: 33
aligned_quality: 2
aligned_quality: 10
aligned_quality: 23
aligned_quality: 27
aligned_quality: 2
aligned_quality: 10
aligned_quality: 23
aligned_quality: 2
aligned_quality: 10
aligned_quality: 23
aligned_quality: 30
aligned_quality: 30
aligned_quality: 31
aligned_quality: 2
aligned_quality: 10
aligned_quality: 23
aligned_quality: 27
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1205:1617:65287"
proper_placement: true
fragment_length: -383
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091785
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "CATAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAAAAGAAATTATGTCTGAA"
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 36
aligned_quality: 37
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091550
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:2203:10845:86770"
proper_placement: true
fragment_length: -470
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091787
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAAAAGAAATTATGTCTGAAAA"
aligned_quality: 25
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 29
aligned_quality: 20
aligned_quality: 35
aligned_quality: 36
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 33
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 35
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 31
aligned_quality: 34
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091465
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1207:5151:86995"
proper_placement: true
fragment_length: 1001
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091787
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TAATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGCCAAACTAAAGACGTCTCTTTCTAAAGTTCACCTGCAGGATCTGAAAATGATTACAAAAGAAATTATGTCTGATAA"
aligned_quality: 7
aligned_quality: 7
aligned_quality: 26
aligned_quality: 7
aligned_quality: 12
aligned_quality: 13
aligned_quality: 8
aligned_quality: 13
aligned_quality: 13
aligned_quality: 7
aligned_quality: 7
aligned_quality: 24
aligned_quality: 19
aligned_quality: 25
aligned_quality: 26
aligned_quality: 28
aligned_quality: 8
aligned_quality: 28
aligned_quality: 7
aligned_quality: 31
aligned_quality: 29
aligned_quality: 8
aligned_quality: 13
aligned_quality: 23
aligned_quality: 31
aligned_quality: 23
aligned_quality: 27
aligned_quality: 8
aligned_quality: 17
aligned_quality: 27
aligned_quality: 23
aligned_quality: 8
aligned_quality: 31
aligned_quality: 29
aligned_quality: 30
aligned_quality: 30
aligned_quality: 28
aligned_quality: 28
aligned_quality: 29
aligned_quality: 29
aligned_quality: 30
aligned_quality: 30
aligned_quality: 28
aligned_quality: 21
aligned_quality: 16
aligned_quality: 25
aligned_quality: 8
aligned_quality: 21
aligned_quality: 18
aligned_quality: 27
aligned_quality: 29
aligned_quality: 30
aligned_quality: 25
aligned_quality: 24
aligned_quality: 7
aligned_quality: 18
aligned_quality: 16
aligned_quality: 13
aligned_quality: 18
aligned_quality: 20
aligned_quality: 12
aligned_quality: 26
aligned_quality: 8
aligned_quality: 27
aligned_quality: 29
aligned_quality: 29
aligned_quality: 26
aligned_quality: 8
aligned_quality: 26
aligned_quality: 21
aligned_quality: 26
aligned_quality: 29
aligned_quality: 8
aligned_quality: 7
aligned_quality: 23
aligned_quality: 8
aligned_quality: 8
aligned_quality: 7
aligned_quality: 26
aligned_quality: 23
aligned_quality: 23
aligned_quality: 23
aligned_quality: 7
aligned_quality: 16
aligned_quality: 7
aligned_quality: 11
aligned_quality: 26
aligned_quality: 6
aligned_quality: 19
aligned_quality: 7
aligned_quality: 20
aligned_quality: 21
aligned_quality: 26
aligned_quality: 20
aligned_quality: 22
aligned_quality: 22
aligned_quality: 7
aligned_quality: 7
aligned_quality: 22
aligned_quality: 28
aligned_quality: 28
aligned_quality: 21
aligned_quality: 7
aligned_quality: 7
aligned_quality: 20
aligned_quality: 17
aligned_quality: 7
aligned_quality: 7
aligned_quality: 18
aligned_quality: 21
aligned_quality: 7
aligned_quality: 7
aligned_quality: 7
aligned_quality: 10
aligned_quality: 21
aligned_quality: 20
aligned_quality: 17
aligned_quality: 21
aligned_quality: 25
aligned_quality: 14
aligned_quality: 7
aligned_quality: 7
aligned_quality: 7
aligned_quality: 7
aligned_quality: 7
aligned_quality: 21
aligned_quality: 7
aligned_quality: 10
aligned_quality: 20
aligned_quality: 7
aligned_quality: 23
aligned_quality: 14
aligned_quality: 7
aligned_quality: 7
aligned_quality: 10
aligned_quality: 23
aligned_quality: 17
aligned_quality: 21
aligned_quality: 20
aligned_quality: 21
aligned_quality: 17
aligned_quality: 7
aligned_quality: 23
aligned_quality: 21
aligned_quality: 25
aligned_quality: 7
aligned_quality: 18
aligned_quality: 25
next_mate_position {
  reference_name: "20"
  position: 38092640
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1205:11612:39712"
proper_placement: true
fragment_length: -474
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091788
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AATAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAAAAGAAATTATGTCTGAAAAA"
aligned_quality: 31
aligned_quality: 31
aligned_quality: 25
aligned_quality: 27
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 31
aligned_quality: 25
aligned_quality: 34
aligned_quality: 32
aligned_quality: 25
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 29
aligned_quality: 20
aligned_quality: 15
aligned_quality: 23
aligned_quality: 18
aligned_quality: 24
aligned_quality: 34
aligned_quality: 33
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 29
aligned_quality: 31
aligned_quality: 29
aligned_quality: 26
aligned_quality: 12
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 36
aligned_quality: 35
aligned_quality: 33
aligned_quality: 26
aligned_quality: 36
aligned_quality: 33
aligned_quality: 35
aligned_quality: 34
aligned_quality: 29
aligned_quality: 32
aligned_quality: 20
aligned_quality: 40
aligned_quality: 39
aligned_quality: 36
aligned_quality: 38
aligned_quality: 36
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 35
aligned_quality: 31
aligned_quality: 37
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 36
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 32
aligned_quality: 32
aligned_quality: 30
aligned_quality: 29
aligned_quality: 32
aligned_quality: 33
aligned_quality: 35
aligned_quality: 27
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 30
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091462
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:2203:7369:35760"
proper_placement: true
fragment_length: -467
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091790
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAAAAGAAATTATGTCTGAAAAAGG"
aligned_quality: 31
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 33
aligned_quality: 32
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 33
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 37
aligned_quality: 34
aligned_quality: 29
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 35
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 34
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 36
aligned_quality: 37
aligned_quality: 35
aligned_quality: 30
aligned_quality: 34
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091471
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1114:18674:74455"
proper_placement: true
fragment_length: 276
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091790
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TAACAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAAAAGAAATTATGTCTGAAAAAGG"
aligned_quality: 34
aligned_quality: 33
aligned_quality: 34
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 30
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38091918
  reverse_strand: true
}

fragment_name: "HISEQ1:23:H9UD5ADXX:2:1214:20239:5891"
proper_placement: true
fragment_length: 657
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091793
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "CAAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAAAAGAAATTATGTCTGAAAAAGGAGC"
aligned_quality: 31
aligned_quality: 27
aligned_quality: 31
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 38
aligned_quality: 39
aligned_quality: 35
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 36
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 31
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 34
aligned_quality: 36
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 35
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 36
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 35
aligned_quality: 40
aligned_quality: 36
aligned_quality: 35
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 36
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 36
aligned_quality: 28
aligned_quality: 32
aligned_quality: 36
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 23
aligned_quality: 27
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 33
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 29
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
next_mate_position {
  reference_name: "20"
  position: 38092302
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:2114:4019:67696"
proper_placement: true
fragment_length: -366
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091794
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAAAAGAAATTATGTCTGAAAAAGGAGCT"
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 31
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 31
aligned_quality: 33
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091576
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1210:18770:99355"
proper_placement: true
fragment_length: -349
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091794
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAAAAGAAATTATGTCTGAAAAAGGAGCT"
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 25
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 29
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 27
aligned_quality: 29
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 31
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 32
aligned_quality: 36
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 30
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 38
aligned_quality: 36
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 36
aligned_quality: 36
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 32
aligned_quality: 32
aligned_quality: 34
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 38
aligned_quality: 30
aligned_quality: 38
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091593
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1114:15096:7766"
proper_placement: true
fragment_length: -533
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091794
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AAACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAAAAGAAATTATGTCTGAAAAAGGAGCT"
aligned_quality: 32
aligned_quality: 29
aligned_quality: 29
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 31
aligned_quality: 33
aligned_quality: 25
aligned_quality: 25
aligned_quality: 31
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 29
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 27
aligned_quality: 34
aligned_quality: 34
aligned_quality: 30
aligned_quality: 32
aligned_quality: 24
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 31
aligned_quality: 31
aligned_quality: 29
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 29
aligned_quality: 29
aligned_quality: 26
aligned_quality: 29
aligned_quality: 31
aligned_quality: 31
aligned_quality: 30
aligned_quality: 31
aligned_quality: 33
aligned_quality: 33
aligned_quality: 32
aligned_quality: 36
aligned_quality: 39
aligned_quality: 34
aligned_quality: 32
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 37
aligned_quality: 33
aligned_quality: 32
aligned_quality: 40
aligned_quality: 40
aligned_quality: 36
aligned_quality: 39
aligned_quality: 36
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 37
aligned_quality: 27
aligned_quality: 29
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 37
aligned_quality: 33
aligned_quality: 36
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 34
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 39
aligned_quality: 33
aligned_quality: 38
aligned_quality: 37
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091409
}

fragment_name: "HISEQ1:22:H9UJNADXX:2:1111:13451:59037"
proper_placement: true
fragment_length: -572
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091795
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "AACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAAAAGAAATTATGTCTGAAAAAGGAGCTG"
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 30
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 32
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 29
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 36
aligned_quality: 33
aligned_quality: 33
aligned_quality: 39
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 37
aligned_quality: 37
aligned_quality: 38
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 29
aligned_quality: 39
aligned_quality: 38
aligned_quality: 39
aligned_quality: 39
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 35
aligned_quality: 31
aligned_quality: 30
aligned_quality: 31
next_mate_position {
  reference_name: "20"
  position: 38091371
}

fragment_name: "HISEQ1:23:H9UD5ADXX:1:1207:13479:27191"
proper_placement: true
fragment_length: 544
read_number: 1
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091796
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "ACATCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAAAAGAAATTATGTCTGAAAAAGGAGCTGT"
aligned_quality: 31
aligned_quality: 34
aligned_quality: 31
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 36
aligned_quality: 35
aligned_quality: 39
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 41
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 41
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 35
aligned_quality: 39
aligned_quality: 38
aligned_quality: 36
aligned_quality: 39
aligned_quality: 38
aligned_quality: 38
aligned_quality: 40
aligned_quality: 38
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 33
aligned_quality: 39
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 36
aligned_quality: 28
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 39
aligned_quality: 40
aligned_quality: 38
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 37
aligned_quality: 39
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 34
aligned_quality: 39
aligned_quality: 39
aligned_quality: 36
aligned_quality: 39
aligned_quality: 39
aligned_quality: 33
aligned_quality: 36
aligned_quality: 37
aligned_quality: 36
aligned_quality: 34
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 29
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 32
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 34
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 34
aligned_quality: 29
aligned_quality: 31
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 32
aligned_quality: 31
aligned_quality: 27
aligned_quality: 33
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
next_mate_position {
  reference_name: "20"
  position: 38092192
  reverse_strand: true
}

fragment_name: "HISEQ1:22:H9UJNADXX:1:1110:3389:8225"
proper_placement: true
fragment_length: -394
number_reads: 2
alignment {
  position {
    reference_name: "20"
    position: 38091799
    reverse_strand: true
  }
  mapping_quality: 60
  cigar {
    operation: ALIGNMENT_MATCH
    operation_length: 148
  }
}
aligned_sequence: "TCAACAGTTAAAATGTCTGCTAATTTGCAGTTAGCCAAAAACAAAAAACATACTTCAAAGTCAAACTAAAGACGTCTCTTTCTAAAGTTCCCCTGCAGGATCTGAAAATGATTAAAAAAGAAATTATGTCTGAAAAAGGAGCTGTTTC"
aligned_quality: 29
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 36
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 33
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 37
aligned_quality: 35
aligned_quality: 35
aligned_quality: 34
aligned_quality: 32
aligned_quality: 34
aligned_quality: 34
aligned_quality: 32
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 34
aligned_quality: 35
aligned_quality: 35
aligned_quality: 36
aligned_quality: 35
aligned_quality: 35
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 39
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 41
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 40
aligned_quality: 40
aligned_quality: 41
aligned_quality: 40
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 39
aligned_quality: 38
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 37
aligned_quality: 34
aligned_quality: 34
aligned_quality: 34
next_mate_position {
  reference_name: "20"
  position: 38091553
}