################################################################################
# VOLCANO PLOTS
################################################################################
#_______________________________________________________________________________
## color by number of sign concentration steps / one dot per drug
#-------------------------------------------------------------------------------
## MAIN FUNCTION
ggvolc2 = function(df, title, Ycut, color=NA, xlab, maxX, maxY, expY, hghBox,
axisMarkY, breaksX, arrowLength, Xhang, minConc, fixedHght) {
# quiets concerns of R CMD check "no visible binding for global variable"
X=NULL; Y=NULL; labX=NULL; labY=NULL; Label=NULL;
hjust=NULL; x=NULL; y=NULL; xend=NULL; yend=NULL;
Color=NULL; .x=NULL; Fill=NULL
# color palette
pal = setNames(rev(c(brewer.pal(11, "RdYlBu")[1:5], "#000000",
brewer.pal(11, "RdBu")[7:11])), nm=1:11)
# check if dataq.frame have required columns
stopifnot(all(c("X","Y","Label","Grey") %in% colnames(df)))
# check if colors are for the palette # if not use the default
col.default = c(pal[2], pal[9])
color = if(all(color %in% colors())) color else col.default
# x axis
Xlims = c(-maxX, maxX)
# y axis
Ylims = c(-0.05, maxY)
# direction of the effect
df$Direction = 0
df$Direction[df$Y>=Ycut & df$X<0] = -1
df$Direction[df$Y>=Ycut & df$X>0] = 1
# add column saying if association is significant
df$IsSignificant = df$Y>=Ycut & !df$Grey
# decide what should have label
df$haveLabel = df$IsSignificant & df$signConc >= minConc
# positions for labels
calcY = function(y.org) {
rng = c(Ycut+0.1,maxY-0.1)
if(length(y.org)==1) {
y.org = rng[1]+(rng[2]-rng[1])/2
} else {
inc = (rng[2]-rng[1])/length(y.org)
newY = rng[1]+inc*(1:length(y.org))
y.org[order(y.org)] = newY
}
y.org
}
df$labY[df$haveLabel] = calcY(y.org=df$Y[df$haveLabel])
df$labX = with(df, ifelse(haveLabel,
ifelse(Direction==1, Xlims[2]-Xhang,
Xlims[1]+Xhang), NA))
df$hjust = ifelse(sign(df$labX)==1, 0, 1)
labNo = length(df$Y[df$haveLabel])
df$Color = factor(6+(df$signConc*df$Direction), levels=1:11)
# make grey dots really grey
df$Color[df$Grey] = 6
# construct the plot
if(sum(df$haveLabel)>0) {
# construct the labels
df2 = df[df$haveLabel,]
gg = ggplot() +
geom_segment(data=df2, aes(x=X, y=Y, xend=labX, yend=labY),
colour="darkgrey", alpha=0.7, linetype="longdash", size=0.1) +
geom_text(data=df2, mapping=aes(x=labX, y=labY, label=Label, hjust=hjust),
size=2.5) # dotted, size=2.5
# and arrows
gg = gg +
geom_segment(data=data.frame(x=0, y=0, xend=-arrowLength, yend=0),
aes(x=x,y=y,xend=xend,yend=yend),
arrow=arrow(length = unit(0.25, "cm"), type="open"),
colour=color[1], size=0.8) +
geom_segment(data=data.frame(x=0, y=0, xend=arrowLength, yend=0),
aes(x=x,y=y,xend=xend,yend=yend),
arrow=arrow(length = unit(0.25, "cm"), type="open"),
colour=color[2], size=0.8)
} else {
gg = ggplot()
}
gg = gg + geom_hline(yintercept=Ycut, colour="dimgrey", linetype="dashed") +
geom_vline(xintercept=0, colour="dimgrey", size=0.1) +
geom_point(data=df, aes(x=X, y=Y, fill=Color, color=Color), shape=21,
size=3) +
scale_fill_manual(values=col2hex(pal, alpha=0.7)) +
scale_color_manual(values=pal) + theme_bw() +
xlab(xlab) + ggtitle(title) +
scale_y_continuous(
expression(italic(p)*"-value"), breaks=seq(0,maxY,axisMarkY),
labels=math_format(expr=10^.x)(-seq(0,maxY,axisMarkY)), limits=Ylims,
expand = c(expY,0)) +
scale_x_continuous(limits=Xlims, breaks=breaksX, labels=percentAxisScale) +
theme(axis.text.y=element_text(size=8), axis.text.x=element_text(size=8),
axis.title.x=element_text(size=8), axis.title.y=element_text(size=8),
plot.title= element_text(size=8))
# construct the gtable
wdths = c(0.2, 0.4, 3.5*(Xlims[2]-Xlims[1]), 0.2)
hghts = c(0.3, ifelse(is.na(fixedHght), hghBox*nrow(df2), fixedHght), 0.3, 0.2)
gt = gtable(widths=unit(wdths, "in"), heights=unit(hghts, "in"))
## make grobs
ggr = ggplotGrob(gg)
## fill in the gtable
gt = gtable_add_grob(gt, gtable_filter(ggr, "panel"), 2, 3)
gt = gtable_add_grob(gt, ggr$grobs[[whichInGrob(ggr, "axis-l")]], 2, 2)
gt = gtable_add_grob(gt, ggr$grobs[[whichInGrob(ggr, "axis-b")]], 3, 3)
gt = gtable_add_grob(gt, ggr$grobs[[whichInGrob(ggr, "xlab-b")]], 4, 3)
gt = gtable_add_grob(gt, ggr$grobs[[whichInGrob(ggr, "ylab-l")]], 2, 1)
gt = gtable_add_grob(gt, gtable_filter(ggr, "title"), 1, 3) # title
# legend # it should adjust depending on minConc
pal = setNames(pal[-ceiling(length(pal)/2)], nm=c(-5:-1,1:5))
fakeDF = data.frame(X=1:length(pal), Y=LETTERS[1:length(pal)], Fill=names(pal))
gl = ggplot() +
geom_bar(data=fakeDF, aes(x=X, y=Y, fill=Fill),
stat="identity", position="identity") +
scale_fill_manual(name="Number of\nsignificant\nconcentrations",
values=pal,
labels=c(`-5`="", `-4`="", `-3`="", `-2`="", `-1`="",
`1`="1 conc.", `2`="2 conc.", `3`="3 conc.",
`4`="4 conc.", `5`="5 conc"), drop = FALSE) +
guides(fill=guide_legend(ncol=2)) +
theme(legend.text=element_text(size=8),
legend.title=element_text(size=8, face="bold"),
legend.title.align=0.5)
# construct the gtable
wdthsL = c(2)
hghtsL = 2
gtL = gtable(widths=unit(wdthsL, "in"), heights=unit(hghtsL, "in"))
## make grobs
ggl = ggplotGrob(gl)
## fill in the gtable
gtL = gtable_add_grob(gtL, ggl$grobs[[whichInGrob(ggl, "guide-box-right")]], 1, 1)
return(list("figure"=list(width=sum(wdths), height=sum(hghts), plot=gt),
"legend"=list(width=sum(wdthsL), height=sum(hghtsL), plot=gtL)))
}
## RUNNING FUNCTION
run.ggvolcGr2 = function(results, effects, screen, mts, fdr, maxX, maxY,
expY, hghBox, axisMarkY, breaksX, arrowLength,
Xhang, minConc, dtab=BloodCancerMultiOmics2017::drugs, fixedHght=NA) {
# select appropriate data to plot
results = results[[screen]]
effects = effects[[screen]]
# merge the results and effects
reseff = merge(results, effects, by=c("DrugID","TestFac","FacDr"))
# filter the data to only those lines which will be plotted
reseff = reseff[reseff$TestFac %in% mts,]
# mark significant cases
reseff$fdr = reseff$adj.pval <= fdr
# find out the p-value threshold
cut = max(reseff$pval[reseff$fdr])
# iterate on each mutation
waste = tapply(1:nrow(reseff), reseff$TestFac, function(idx) {
# mutation to be plotted
mt = reseff[idx[1], "TestFac"]
# select the data to be plotted
re = reseff[idx,]
## for each drug select the association with the biggest difference in effects
re = do.call(rbind, tapply(1:nrow(re), re$DrugID2, function(i) {
tmp = re[i,]
if(all(tmp$fdr==FALSE)) {
return(cbind(tmp[which.max(abs(tmp$WM)),], signConc=0))
} else {
tmp = tmp[tmp$fdr,]
return(cbind(tmp[which.max(abs(tmp$WM)),], signConc=nrow(tmp)))
}
}))
# create input data frame
plotDF = with(re, data.frame(X=(-1)*WM, Y=-log10(pval),
Label=dtab[DrugID2,"name"],
Grey= mean.0 <0.1 & mean.1 <0.1 & fdr,
signConc))
# maxY
if(is.na(maxY)) maxY=max(ceiling(plotDF$Y))
# additional paremeters (labels, colors)
xlab = "Difference of effects"
# run the plotting function
ggvolc2(df=plotDF, title=mt, Ycut=-log10(cut), color=NA, xlab=xlab,
maxX=maxX, maxY=maxY, expY=expY, hghBox=hghBox,
axisMarkY=axisMarkY, breaksX=breaksX, arrowLength=arrowLength,
Xhang=Xhang, minConc=minConc, fixedHght=fixedHght)
})
# names(waste) = paste(names(waste), screen, sep=".")
waste
}
################################################################################
# HEAT MAP
################################################################################
ggheat = function(results, effects, dtab=BloodCancerMultiOmics2017::drugs, ctab=BloodCancerMultiOmics2017::conctab) {
# quiets concerns of R CMD check "no visible binding for global variable"
diag.conc=NULL; Drug2=NULL; Fill=NULL; x=NULL; fill=NULL
# COLORS
# color palette
pal = c(brewer.pal(11, "PiYG")[2], brewer.pal(11, "RdBu")[10]) # only 2 colors!
# border of whole plot
cPanb = "black"
sPanb = 0.5
# minor & major grid
cGrid = c("grey", "dimgrey")
# apply the FDR threshold
FDR = 0.1
plotDF = do.call(rbind, lapply(names(results), function(nm) {
df = merge(results[[nm]], effects[[nm]], by=c("FacDr", "TestFac", "DrugID"))
df$plot.pval = ifelse(df$adj.pval <= FDR, df$pval*sign(df$WM), 1)
df$plot.simple.pval = ifelse(df$adj.pval <= FDR, 1*sign(df$WM), 0)
df$plot.simple.pval = factor(df$plot.simple.pval, levels=c(-1,1,0))
df
}))
# add column which orders the coulumns of the plot
plotDF$diag = sapply(plotDF$TestFac, function(tf)
strsplit(tf, ".", fixed=TRUE)[[1]][[2]])
plotDF$conc = sapply(plotDF$DrugID, function(drid) {
splits = strsplit(drid, "-")[[1]]
colnames(ctab)[which(ctab[splits[1],] == splits[2])]
})
plotDF$diag.conc = paste(plotDF$diag, plotDF$conc, sep=".")
diagOrder = names(colDiagS)[names(colDiagS) %in% unique(plotDF$diag)]
plotDF$diag.conc = factor(plotDF$diag.conc,
levels=as.vector(sapply(diagOrder,
function(d)
paste(d, paste0("c", 1:5),
sep="."))))
# names of drugs / order them
plotDF$Drug2 = dtab[plotDF$DrugID2, "name"]
plotDF$Drug2 = factor(plotDF$Drug2, levels=dtab[rev(mainDrOrd),"name"])
# data frame for vline
vltab = table(sapply(unique(plotDF$diag), function(x) grep(x, diagAmt)))
vldf = data.frame(x=seq(5.5, length(levels(plotDF$diag.conc)), 5), col=1)
vldf$col[cumsum(vltab[1:(length(vltab)-1)])] = 2 # remove latest
vldf$col = factor(vldf$col, levels=c(1,2))
plotDF$Fill = log10div(plotDF$plot.pval) # transform the values to log10 scale
plotDF$Fill = pmax(pmin(plotDF$Fill, 12), -12) # censore
# color palette
pal = rev(c(brewer.pal(11,"PiYG")[1:6], brewer.pal(11,"RdBu")[7:11]))
pal[6] = "gray95"
gMain2 = ggplot() + geom_tile(data=plotDF,
aes(x=diag.conc, y=Drug2, fill=Fill)) +
scale_fill_gradientn(expression(italic(p)*"-value"),
colours=pal, limits=c(-12, 12),
breaks=c(-12,-8,-4,0,4,8,12), labels=exp10div) +
theme_bw() +
geom_vline(xintercept=seq(0.5, length(levels(plotDF$diag.conc))+1, 1),
color="white", size=1.5) +
geom_hline(yintercept=seq(0.5, length(levels(plotDF$Drug2))+1, 1),
color="white", size=1.5) +
geom_vline(data=vldf, aes(xintercept=x, color=col)) +
scale_color_manual(values=c("1"=cGrid[1], "2"=cGrid[2])) +
coord_equal() + xlab("") + ylab("") +
scale_y_discrete(expand=c(0,0)) +
scale_x_discrete(expand=c(0,0)) +
theme(axis.title=element_blank(),
axis.text.x=element_blank(),
axis.text.y=element_text(size=10),
axis.ticks.x=element_blank(),
panel.border=element_rect(colour=cPanb, fill=NA, size=sPanb),
legend.key=element_rect(color="black"),
legend.text=element_text(size=10),
legend.title=element_text(size=10, face="bold")) +
guides(color=FALSE)
#################################################
# concentrations
concDF = data.frame(x=factor(levels(plotDF$diag.conc)),
fill=factor(rep(paste0("c",1:5)), levels=paste0("c",1:5)))
concpal = setNames(paste0("grey", seq(35, 95, 15)), nm=paste0("c",1:5))
gConc = ggplot() + geom_tile(data=concDF, aes(x=x, y=1, fill=fill)) +
scale_fill_manual("Drug\nconcentration", values=concpal) +
scale_y_continuous(expand=c(0,0)) +
scale_x_discrete(expand=c(0,0)) + theme_bw() +
theme(axis.title=element_blank(), axis.text=element_blank(),
axis.ticks=element_blank(), legend.text=element_text(size=10),
legend.title=element_text(size=10, face="bold"),
legend.key=element_rect(color="black"),
panel.border=element_rect(colour=cPanb, fill=NA, size=sPanb)) +
geom_vline(data=vldf, aes(xintercept=x, color=col)) +
scale_color_manual(values=c("1"=cGrid[1], "2"=cGrid[2])) +
guides(color=FALSE)
# annotation of cell of origin
diagOrd = sapply(unique(plotDF$diag), function(x) grep(x, diagAmt))
diagOrd = diagOrd[diagOrder]
annoDF = data.frame(x=names(diagAmt)[diagOrd],
diag=factor(names(diagOrd), levels=names(diagOrd)))
annoDF$x = factor(annoDF$x, levels=unique(annoDF$x))
# vline
vldf2 = data.frame(x=seq(1.5, length(levels(annoDF$diag)), 1), col=1)
vldf2$col[cumsum(table(diagOrd)[1:(length(table(diagOrd))-1)])] = 2
vldf2$col = factor(vldf2$col, levels=c(1,2))
gAnno = ggplot() + geom_tile(data=annoDF, aes(x=diag, y=1, fill=x)) +
scale_fill_manual("Disease origin", values=colDiagL) +
scale_y_continuous(expand=c(0,0)) + scale_x_discrete(expand=c(0,0)) +
theme_bw() +
theme(axis.title=element_blank(),
axis.text=element_blank(),
axis.ticks=element_blank(),
legend.text=element_text(size=10),
legend.title=element_text(size=10, face="bold"),
panel.border=element_rect(colour=cPanb, fill=NA, size=sPanb),
legend.key=element_rect(color="black")) +
geom_vline(data=vldf2, aes(xintercept=x, color=col)) +
scale_color_manual(values=c("1"=cGrid[1], "2"=cGrid[2])) +
guides(color=FALSE) +
geom_text(data=annoDF, aes(label=diag, y=1, x=diag))
## MERGE PLOTS INTO ONE FIGURE
# prepare grobs
ggM = ggplotGrob(gMain2)
ggA = ggplotGrob(gAnno)
ggC = ggplotGrob(gConc)
# prepare gtable
nX = length(levels(plotDF$diag.conc))
nY = length(levels(plotDF$Drug2))
unM = 0.15 # unit for main heat map
unA = 0.25 # unit for y-axis of the annotation - diagnosis
unC = 0.15 # unit for y-axis of the concentration
sp = 0.0 # space
wdths = c(1.5, nX*unM, 2)
hghts = c(unA, sp, nY*unM, unC)
gt = gtable(widths=unit(wdths, "in"), heights=unit(hghts, "in"))
# add pieces to gtable
gt = gtable_add_grob(gt, ggA$grobs[[whichInGrob(ggA, "panel")]], 1, 2)
gt = gtable_add_grob(gt, ggM$grobs[[whichInGrob(ggM, "panel")]], 3, 2)
gt = gtable_add_grob(gt, ggC$grobs[[whichInGrob(ggC, "panel")]], 4, 2)
gt = gtable_add_grob(gt, ggM$grobs[[whichInGrob(ggM, "axis-l")]], 3, 1)
# legend cell origin & concentration & effect
wdthsL = c(2,2,2)
hghtsL = 2
gtL = gtable(widths=unit(wdthsL, "in"), heights=unit(hghtsL, "in"))
gtL = gtable_add_grob(gtL, ggA$grobs[[whichInGrob(ggA, "guide-box-right")]], 1, 1)
gtL = gtable_add_grob(gtL, ggM$grobs[[whichInGrob(ggM, "guide-box-right")]], 1, 2)
gtL = gtable_add_grob(gtL, ggC$grobs[[whichInGrob(ggC, "guide-box-right")]], 1, 3)
return(list("figure"=list(width=sum(wdths), height=sum(hghts), plot=gt),
"legend"=list(width=sum(wdthsL), height=sum(hghtsL), plot=gtL)))
}
################################################################################
# BEE SWARM PLOTS
################################################################################
beeF <- function(drug, mut, cs, diag, y1, y2, custc,
lpd=BloodCancerMultiOmics2017::lpdAll,
ctab=BloodCancerMultiOmics2017::conctab,
dtab=BloodCancerMultiOmics2017::drugs) {
col1 <- vector(); col2 <- vector()
dr <- lpd[ , lpd$Diagnosis %in% diag ]
p = t.test( Biobase::exprs(dr)[drug,] ~ Biobase::exprs(dr)[mut,], var.equal = TRUE)$p.value
#clonsize
af <- fData(BloodCancerMultiOmics2017::mutCOM)[colnames(dr), paste0(mut, "cs")]
#Create a function to generate a continuous color palette
rbPal <- colorRampPalette(c('coral1','blue4'))
# This adds a cont. color code
if (cs==T) {
col2 <- rbPal(100)[as.numeric(cut( af, breaks = 100 ))]
} else {
col2 <- "blue4"
}
if (custc==T) {
col1 <- ifelse(Biobase::exprs(dr)[mut,]==1, col2,"coral1")
} else {
col1 <- ifelse(Biobase::exprs(dr)[mut,]==1, "green","magenta")
}
beeswarm( Biobase::exprs(dr)[drug,] ~ Biobase::exprs(dr)[mut,],
method = 'swarm',
pch = 19, pwcol = col1, cex = 1.6,
xlab = '', ylab = 'Viability', cex.axis=1.6, cex.lab=1.9,
ylim=c(y1,y2),
labels = c("AF=0", "AF>0"),
main=(paste0( giveDrugLabel(drug, ctab, dtab), " ~ ", mut,
"\n (p = ",
digits = format.pval(p,
max(1, getOption("digits") - 4)),
")")),
cex.main=2.0,
bty="n"
)
boxplot(Biobase::exprs(dr)[drug,] ~ Biobase::exprs(dr)[mut,], add = T, names = c("",""),
col="#0000ff22", axes = 0, outline=FALSE)
}
# BEESWARM FOR PRETREATMENT
beePretreatment = function(lpd, drug, y1, y2, fac, val, name) {
dr <- lpd[ , Biobase::exprs(lpd)[fac,] %in% val]
pretreat <- BloodCancerMultiOmics2017::patmeta[colnames(dr),
"IC50beforeTreatment"]
p = t.test( Biobase::exprs(dr)[drug,] ~ pretreat, var.equal = TRUE)$p.value
beeswarm( Biobase::exprs(dr)[drug,] ~ pretreat,
method = 'swarm',
pch = 19, cex = 1.2, pwcol=ifelse(pretreat, "blue4", "coral1"),
xlab = '', ylab = 'Viability', cex.axis=1.6, cex.lab=1.8,
labels = c("pre-treated", "not treated"),
main=paste0(
name," (p = ", digits = format.pval(
p, max(1, getOption("digits") - 4)), ")"), ylim=c(y1,y2),
cex.main=2,
bty="n"
)
boxplot(Biobase::exprs(dr)[drug,] ~ pretreat, add = T, names = c("",""),
col="#0000ff22", axes = 0, outline=FALSE)
}
Datasets
Models
