--- a
+++ b/BioAid/diluter.py
@@ -0,0 +1,78 @@
+# This code was developed and authored by Jerzy Twarowski in Malkova Lab at the University of Iowa 
+# Contact: jerzymateusz-twarowski@uiowa.edu, tvarovski1@gmail.com
+
+def calculate_dilution(count_per_square: int|float, final_plate: int|float) -> None:
+    """
+    Calculates the dilution factor and dilution amounts for a given cell count and final plate volume.
+
+    Args:
+        count_per_square (float): The number of cells counted per square on a hemocytometer.
+        final_plate (float): The desired number of cells per plate.
+
+    Returns:
+        None
+
+    Examples:
+        >>> calculate_dilution(50, 100)
+        
+        You should dilute by using 0.25 of cell culture with 0.75uL ddH2O 
+        and then with three steps of 100uL of diluted culture and 900uL ddH2O.
+        Plate with 100uL of diluted solution per plate
+    """
+    init_cells_ml = (count_per_square/4.0)*10**6
+    final_cells_ml = final_plate*10
+
+    dilution_factor = init_cells_ml/final_cells_ml
+    dilution_factor = dilution_factor/1000
+
+    fourth_dilution_amount = 1000/dilution_factor
+
+    print(f"\nYou should dilute by using {fourth_dilution_amount} of cell culture with {1000-fourth_dilution_amount}uL ddH2O \nand then with three steps of 100uL of diluted culture and 900uL ddH2O.\nPlate with 100uL of diluted solution per plate")
+
+def dilute() -> None:
+    """
+    Prompts the user for input and calculates the dilution factor and dilution amounts using the calculate_dilution function.
+
+    Args:
+        None
+
+    Returns:
+        None
+
+    Raises:
+        None
+
+    Examples:
+        >>> dilute()
+
+        Welcome to the Diluter 1.0. Enjoy not doing the math yourself!
+
+        Initial number of cells refers to the average number of cells on the 1/16th of hematocrit slide.
+        For best results take the average of at least three of these squares.
+
+        Type initial number of cells in media or enter 'x' to exit: 50
+
+        Type in desired number of colonies per plate (100uL of media): 100
+
+        You should dilute by using 0.25 of cell culture with 0.75uL ddH2O 
+        and then with three steps of 100uL of diluted culture and 900uL ddH2O.
+        Plate with 100uL of diluted solution per plate
+    """
+
+    welcome_message = "\nWelcome to the Diluter 1.0. Enjoy not doing the math yourself!\n"
+    print(welcome_message)
+
+    print("Initial number of cells refers to the average number of cells on the 1/16th of hematocrit slide.\nFor best results take the average of at least three of these squares.")
+
+    looping = True
+
+    while looping:
+        initial_cells = input("\nType initial number of cells in media or enter 'x' to exit: ")
+        if initial_cells == "x":
+            looping = False
+            continue
+        initial_cells = float(initial_cells)
+        final_cells = input("\nType in desired number of colonies per plate (100uL of media): ")
+        final_cells = float(final_cells)
+
+        calculate_dilution(initial_cells,final_cells)
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