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# Getting Started |
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A [video tutorial](https://youtu.be/dOwkInn6eDw) covering the instructions below is also available. |
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### <a name="system-requirements"></a>System Requirements |
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* JDK [17](https://www.oracle.com/java/technologies/downloads/#jdk17-windows)+ |
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* A `JAVA_HOME` environment variable pointing to your JDK, or the Java executable in your PATH |
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* [Gradle](https://gradle.org/install/) (included) |
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### <a name="build-run"></a>Build & Run |
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The easiest way to run the program on Windows is by using the executable file located in the [releases](https://github.com/VerisimilitudeX/DNAnalyzer/releases/latest) section to install the program, build gradle and run the GUI. |
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* We use [Gradle](https://gradle.org) for building. The Gradle wrapper takes care of downloading dependencies, testing, compiling, linking, and packaging the code. |
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<details> |
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<summary>Windows</summary> |
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The easiest way to run the program on Windows is by using the executable file located in the [releases](https://github.com/VerisimilitudeX/DNAnalyzer/releases/latest) section to install the program, build gradle and run the GUI. |
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```pwsh |
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.\gradlew build |
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``` |
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### <a name="usage"></a>Usage |
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```pwsh |
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<executable> <arguments> |
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``` |
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#### <a name="executable"></a>Executable |
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```pwsh |
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java -jar build/libs/DNAnalyzer.jar |
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``` |
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#### <a name="arguments"></a>Arguments |
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DNAnalyzer uses CLI arguments instead of `stdin`. For example, you can do: |
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```pwsh |
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assets/dna/random/dnalong.fa --amino=arg --min=16450 --max=520218 -r |
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``` |
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### <a name="example"></a>Example |
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```pwsh |
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java -jar build/libs/DNAnalyzer.jar assets/dna/random/dnalong.fa --amino=ser --min=16450 --max=520218 -r |
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``` |
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#### <a name="gradle-run"></a>Gradle Run |
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If you prefer, you can also run it directly from Gradle: |
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```pwsh |
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.\gradlew run --args="assets/dna/random/dnalong.fa --amino=ser --min=10 --max=100" |
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``` |
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#### <a name="gui"></a>GUI |
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DNAnalyzer also comes with a (very basic) GUI; to start DNAnalyzer with the GUI, run: |
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```pwsh |
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.\gradlew run --args="--gui assets/dna/random/dnalong.fa" |
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``` |
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Then: |
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* Enter the file name of the DNA file in the text field |
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* Set min and max |
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* Click analyze |
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* Note: Ensure you have Java [17](https://www.oracle.com/java/technologies/downloads/#java17) or higher installed and a `JAVA_HOME` path variable set for the program to function correctly! |
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The results of your analysis will be shown in the right pane. |
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</details> |
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<details> |
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<summary>Linux & macOS</summary> |
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### <a name="features"></a>DNAnalyzer & Java Download |
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To run DNAnaylzer on Linux, you'll need to download the DNAnalyzer source code and download and install Java 17. |
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First, download the zip or tar.gz file from [DNAnalyzer releases](https://github.com/VerisimilitudeX/DNAnalyzer/releases/latest) and unzip it using the native utility. |
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Then, download [Java 17](https://www.oracle.com/ca-en/java/technologies/downloads/#java17), find the download at https://www.oracle.com/ca-en/java/technologies/downloads/#java17, ensure you choose the Linux option and get the one for your correct processor architecture. |
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### Java installation |
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```bash |
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wget https://download.oracle.com/java/17/latest/jdk-17_linux-x64_bin.tar.gz |
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``` |
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Next, unzip it. |
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```bash |
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tar -xvzf jdk-17_linux-x64_bin.tar.gz |
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``` |
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Then map the `JAVA_HOME` path. Fill it in with your JAVA directory where JAVA is unzipped. |
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```bash |
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export JAVA_HOME="{YOUR JAVA DIRECTORY HERE}/jdk-17.0.7" && export PATH=$JAVA_HOME/bin:$PATH |
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``` |
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<details> |
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<summary>Example of a full path command.</summary> |
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```bash |
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export JAVA_HOME="/workspaces/DNAnalyzer/jdk-17.0.7" |
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``` |
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</details> |
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```bash |
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./gradlew build |
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``` |
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If you see `Task :compileJava FAILED`, the program cannot find your Java installation. You may need to export your JAVA_HOME path again. |
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## OpenAI API Key |
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We use GPT-4 API to display the results of the analysis. To use the API, you'll need to get an API key from OpenAI. You can get one [here](https://platform.openai.com/). **Note, this is an optional step.** If you don't want to use the API, you can still use the program, but you won't be able to see the results of the analysis. |
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To set the API key on Linux or macOS, run `export OPENAI_API_KEY=sk-xxxxxxxxxxxxxxxxxxxxxxxx` where `sk-xxxxxxxxxxxxxxxxxxxxxxxx` is your API key. |
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For Windows, use `setx OPENAI_API_KEY=sk-xxxxxxxxxxxxxxxxxxxxxxxx` instead. |
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### Usage |
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```bash |
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<executable> <arguments> |
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``` |
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#### Executable |
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```pwsh |
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java -jar build/libs/DNAnalyzer.jar |
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``` |
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#### Arguments |
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DNAnalyzer uses CLI arguments instead of `stdin`. For example, you can do: |
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```bash |
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assets/dna/random/dnalong.fa --amino=arg --min=16450 --max=520218 -r |
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``` |
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### Example |
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```pwsh |
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java -jar build/libs/DNAnalyzer.jar assets/dna/random/dnalong.fa --amino=ser --min=16450 --max=520218 -r |
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``` |
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#### Gradle Run |
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If you prefer, you can also run it directly from Gradle: |
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```pwsh |
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./gradlew run --args="assets/dna/random/dnalong.fa --amino=ser --min=10 --max=100" |
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``` |
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#### GUI |
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DNAnalyzer also comes with a (very basic) GUI; to start DNAnalyzer with the GUI, run: |
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```pwsh |
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./gradlew run --args="--gui assets/dna/random/dnalong.fa" |
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``` |
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Then: |
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* Enter the file name of the DNA file in the text field |
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* Set min and max |
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* Click analyze |
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The results of your analysis will be shown in the right pane. |
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</details> |
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#### Available Commands |
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```py |
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Usage: DNAnalyzer [-hrV] --amino=<aminoAcid> [--find=<proteinFile>] |
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[--max=<maxCount>] [--min=<minCount>] DNA |
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A program to analyze DNA sequences. |
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DNA The FASTA file to be analyzed. |
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--amino=<aminoAcid> The amino acid representing the start of a gene. |
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--find=<proteinFile> The DNA sequence to be found within the FASTA file. |
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-h, --help Show this help message and exit. |
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--max=<maxCount> The maximum count of the reading frame. |
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--min=<minCount> The minimum count of the reading frame. |
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-r, --reverse Reverse the DNA sequence before processing. |
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-V, --version Print version information and exit. |
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``` |