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b/tests/test_wsic.py |
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"""Tests for `wsic` package.""" |
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import sys |
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import warnings |
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from pathlib import Path |
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from typing import Dict |
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import cv2 as _cv2 # Avoid adding "cv2" to sys.modules for fallback tests |
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import numpy as np |
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import pytest |
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import tifffile |
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import zarr |
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from wsic import readers, utils, writers |
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from wsic.enums import Codec, ColorSpace |
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from wsic.readers import Reader |
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from wsic.writers import Writer |
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def test_jp2_to_deflate_tiled_tiff(samples_path, tmp_path): |
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"""Test that we can convert a JP2 to a DEFLATE compressed tiled TIFF.""" |
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with warnings.catch_warnings(): |
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warnings.simplefilter("error") |
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reader = readers.Reader.from_file(samples_path / "XYC.jp2") |
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writer = writers.TIFFWriter( |
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path=tmp_path / "XYC.tiff", |
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shape=reader.shape, |
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overwrite=False, |
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tile_size=(256, 256), |
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codec="deflate", |
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microns_per_pixel=(0.5, 0.5), # the input .jp2 has no resolution box |
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) |
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writer.copy_from_reader(reader=reader, num_workers=3, read_tile_size=(512, 512)) |
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assert writer.path.exists() |
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assert writer.path.is_file() |
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assert writer.path.stat().st_size > 0 |
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output = tifffile.imread(writer.path) |
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assert np.all(reader[:512, :512] == output[:512, :512]) |
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def test_jp2_to_deflate_pyramid_tiff(samples_path, tmp_path): |
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"""Test that we can convert a JP2 to a DEFLATE compressed pyramid TIFF.""" |
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pyramid_downsamples = [2, 4] |
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with warnings.catch_warnings(): |
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warnings.simplefilter("error") |
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reader = readers.Reader.from_file(samples_path / "XYC.jp2") |
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writer = writers.TIFFWriter( |
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path=tmp_path / "XYC.tiff", |
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shape=reader.shape, |
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overwrite=False, |
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tile_size=(256, 256), |
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codec="deflate", |
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pyramid_downsamples=pyramid_downsamples, |
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microns_per_pixel=(0.5, 0.5), # the input .jp2 has no resolution box |
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) |
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writer.copy_from_reader(reader=reader, num_workers=3, read_tile_size=(512, 512)) |
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assert writer.path.exists() |
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assert writer.path.is_file() |
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assert writer.path.stat().st_size > 0 |
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output = tifffile.imread(writer.path) |
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assert np.all(reader[:512, :512] == output[:512, :512]) |
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tif = tifffile.TiffFile(writer.path) |
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assert len(tif.series[0].levels) == len(pyramid_downsamples) + 1 |
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def test_no_tqdm(samples_path, tmp_path, monkeypatch): |
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"""Test making a pyramid TIFF with no tqdm (progress bar) installed.""" |
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# Make tqdm unavailable |
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monkeypatch.setitem(sys.modules, "tqdm", None) |
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monkeypatch.setitem(sys.modules, "tqdm.auto", None) |
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# Sanity check the imports fail |
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with pytest.raises(ImportError): |
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import tqdm # noqa |
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with pytest.raises(ImportError): |
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from tqdm.auto import tqdm # noqa |
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pyramid_downsamples = [2, 4] |
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with warnings.catch_warnings(): |
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warnings.simplefilter("error") |
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reader = readers.Reader.from_file(samples_path / "XYC.jp2") |
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writer = writers.TIFFWriter( |
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path=tmp_path / "XYC.tiff", |
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shape=reader.shape, |
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overwrite=False, |
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tile_size=(256, 256), |
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codec="deflate", |
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pyramid_downsamples=pyramid_downsamples, |
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microns_per_pixel=(0.5, 0.5), # the input .jp2 has no resolution box |
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) |
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writer.copy_from_reader(reader=reader, num_workers=3, read_tile_size=(512, 512)) |
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assert writer.path.exists() |
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assert writer.path.is_file() |
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assert writer.path.stat().st_size > 0 |
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output = tifffile.imread(writer.path) |
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assert np.all(reader[:512, :512] == output[:512, :512]) |
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tif = tifffile.TiffFile(writer.path) |
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assert len(tif.series[0].levels) == len(pyramid_downsamples) + 1 |
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def test_pyramid_tiff(samples_path, tmp_path, monkeypatch): |
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"""Test pyramid generation using OpenCV to downsample.""" |
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# Try to make a pyramid TIFF |
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reader = readers.Reader.from_file(samples_path / "XYC.jp2") |
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pyramid_downsamples = [2, 4] |
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writer = writers.TIFFWriter( |
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path=tmp_path / "XYC.tiff", |
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shape=reader.shape, |
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overwrite=False, |
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tile_size=(256, 256), |
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codec="deflate", |
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pyramid_downsamples=pyramid_downsamples, |
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) |
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writer.copy_from_reader( |
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reader=reader, num_workers=3, read_tile_size=(512, 512), downsample_method="cv2" |
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) |
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assert writer.path.exists() |
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assert writer.path.is_file() |
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assert writer.path.stat().st_size > 0 |
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output = tifffile.imread(writer.path) |
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assert np.all(reader[:512, :512] == output[:512, :512]) |
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tif = tifffile.TiffFile(writer.path) |
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assert len(tif.series[0].levels) == len(pyramid_downsamples) + 1 |
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def test_pyramid_tiff_no_cv2(samples_path, tmp_path, monkeypatch): |
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"""Test pyramid generation when cv2 is not installed. |
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This will use SciPy. This method has a high error on synthetic data, |
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e.g. a test grid image. It performns better on natural images. |
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""" |
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# Make cv2 unavailable |
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monkeypatch.setitem(sys.modules, "cv2", None) |
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# Sanity check the import fails |
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with pytest.raises(ImportError): |
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import cv2 # noqa # skipcq |
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# Try to make a pyramid TIFF |
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reader = readers.Reader.from_file(samples_path / "XYC.jp2") |
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pyramid_downsamples = [2, 4] |
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writer = writers.TIFFWriter( |
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path=tmp_path / "XYC.tiff", |
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shape=reader.shape, |
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overwrite=False, |
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tile_size=(256, 256), |
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codec="deflate", |
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pyramid_downsamples=pyramid_downsamples, |
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) |
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writer.copy_from_reader( |
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reader=reader, |
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num_workers=3, |
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read_tile_size=(512, 512), |
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downsample_method="scipy", |
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) |
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assert writer.path.exists() |
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assert writer.path.is_file() |
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assert writer.path.stat().st_size > 0 |
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output = tifffile.imread(writer.path) |
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assert np.all(reader[:512, :512] == output[:512, :512]) |
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tif = tifffile.TiffFile(writer.path) |
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level_0 = tif.series[0].levels[0].asarray() |
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assert len(tif.series[0].levels) == len(pyramid_downsamples) + 1 |
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for level in tif.series[0].levels[:2]: |
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level_array = level.asarray() |
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level_size = level_array.shape[:2][::-1] |
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resized_level_0 = _cv2.resize(level_0, level_size) |
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level_array = _cv2.GaussianBlur(level_array, (11, 11), 0) |
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resized_level_0 = _cv2.GaussianBlur(resized_level_0, (11, 11), 0) |
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mse = ((level_array.astype(float) - resized_level_0.astype(float)) ** 2).mean() |
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assert mse < 200 |
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assert len(np.unique(level_array)) > 1 |
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assert resized_level_0.mean() == pytest.approx(level_array.mean(), abs=5) |
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assert np.allclose(level_array, resized_level_0, atol=50) |
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def test_pyramid_tiff_no_cv2_no_scipy(samples_path, tmp_path, monkeypatch): |
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"""Test pyramid generation when neither cv2 or scipy are installed.""" |
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# Make cv2 and scipy unavailable |
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monkeypatch.setitem(sys.modules, "cv2", None) |
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monkeypatch.setitem(sys.modules, "scipy", None) |
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# Sanity check the imports fail |
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with pytest.raises(ImportError): |
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import cv2 # noqa # skipcq |
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with pytest.raises(ImportError): |
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import scipy # noqa # skipcq |
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# Try to make a pyramid TIFF |
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reader = readers.Reader.from_file(samples_path / "XYC.jp2") |
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pyramid_downsamples = [2, 4] |
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writer = writers.TIFFWriter( |
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path=tmp_path / "XYC.tiff", |
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shape=reader.shape, |
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overwrite=False, |
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tile_size=(256, 256), |
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codec="deflate", |
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pyramid_downsamples=pyramid_downsamples, |
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) |
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writer.copy_from_reader( |
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reader=reader, num_workers=3, read_tile_size=(512, 512), downsample_method="np" |
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) |
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assert writer.path.exists() |
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assert writer.path.is_file() |
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assert writer.path.stat().st_size > 0 |
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output = tifffile.imread(writer.path) |
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assert np.all(reader[:512, :512] == output[:512, :512]) |
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tif = tifffile.TiffFile(writer.path) |
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level_0 = tif.series[0].levels[0].asarray() |
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assert len(tif.series[0].levels) == len(pyramid_downsamples) + 1 |
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# Check that the levels are not blank and have a sensible range |
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for level in tif.series[0].levels[:2]: |
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level_array = level.asarray() |
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level_size = level_array.shape[:2][::-1] |
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resized_level_0 = _cv2.resize(level_0, level_size) |
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mse = ((level_array.astype(float) - resized_level_0.astype(float)) ** 2).mean() |
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assert mse < 10 |
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assert len(np.unique(level_array)) > 1 |
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assert resized_level_0.mean() == pytest.approx(level_array.mean(), abs=1) |
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assert np.allclose(level_array, resized_level_0, atol=1) |
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def test_jp2_to_webp_tiled_tiff(samples_path, tmp_path): |
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"""Test that we can convert a JP2 to a WebP compressed tiled TIFF.""" |
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with warnings.catch_warnings(): |
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warnings.simplefilter("error") |
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reader = readers.Reader.from_file(samples_path / "XYC.jp2") |
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writer = writers.TIFFWriter( |
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path=tmp_path / "XYC.tiff", |
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shape=reader.shape, |
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overwrite=False, |
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tile_size=(256, 256), |
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codec="WebP", |
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compression_level=-1, # <0 for lossless |
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microns_per_pixel=(0.5, 0.5), # input has no resolution box |
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) |
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writer.copy_from_reader(reader=reader, num_workers=3, read_tile_size=(512, 512)) |
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assert writer.path.exists() |
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assert writer.path.is_file() |
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assert writer.path.stat().st_size > 0 |
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output = tifffile.imread(writer.path) |
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assert np.all(reader[:512, :512] == output[:512, :512]) |
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267 |
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def test_jp2_to_zarr(samples_path, tmp_path): |
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"""Convert JP2 to a single level Zarr.""" |
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with warnings.catch_warnings(): |
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warnings.simplefilter("error") |
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reader = readers.Reader.from_file(samples_path / "XYC.jp2") |
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writer = writers.ZarrWriter( |
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path=tmp_path / "XYC.zarr", |
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shape=reader.shape, |
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) |
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writer.copy_from_reader( |
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reader=reader, |
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num_workers=3, |
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read_tile_size=(512, 512), |
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) |
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assert writer.path.exists() |
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assert writer.path.is_dir() |
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assert list(writer.path.iterdir()) |
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287 |
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output = zarr.open(writer.path) |
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assert np.all(reader[:512, :512] == output[0][:512, :512]) |
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290 |
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291 |
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def test_jp2_to_pyramid_zarr(samples_path, tmp_path): |
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"""Convert JP2 to a pyramid Zarr.""" |
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with warnings.catch_warnings(): |
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warnings.simplefilter("error") |
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296 |
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reader = readers.Reader.from_file(samples_path / "XYC.jp2") |
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pyramid_downsamples = [2, 4, 8, 16, 32] |
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writer = writers.ZarrWriter( |
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path=tmp_path / "XYC.zarr", |
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shape=reader.shape, |
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pyramid_downsamples=pyramid_downsamples, |
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tile_size=(256, 256), |
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) |
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writer.copy_from_reader(reader=reader, num_workers=3, read_tile_size=(256, 256)) |
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assert writer.path.exists() |
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assert writer.path.is_dir() |
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assert list(writer.path.iterdir()) |
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310 |
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output = zarr.open(writer.path) |
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assert np.all(reader[:512, :512] == output[0][:512, :512]) |
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313 |
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for level, dowmsample in zip(output.values(), [1] + pyramid_downsamples): |
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assert level.shape[:2] == ( |
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reader.shape[0] // dowmsample, |
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reader.shape[1] // dowmsample, |
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) |
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320 |
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def test_warn_unused(samples_path, tmp_path): |
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"""Test the warning about unsued arguments.""" |
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reader = readers.Reader.from_file(samples_path / "XYC.jp2") |
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with pytest.warns(UserWarning): |
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writers.JP2Writer( |
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path=tmp_path / "XYC.tiff", |
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shape=reader.shape, |
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overwrite=False, |
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tile_size=(256, 256), |
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codec="WebP", |
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compression_level=70, |
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) |
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333 |
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334 |
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def test_read_zarr_array(tmp_path): |
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"""Test that we can open a Zarr array.""" |
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# Create a Zarr array |
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array = zarr.open( |
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tmp_path / "test.zarr", |
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mode="w", |
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shape=(10, 10), |
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chunks=(2, 2), |
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dtype=np.uint8, |
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) |
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array[:] = np.random.randint(0, 255, size=(10, 10)) |
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# Open the array |
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reader = readers.Reader.from_file(tmp_path / "test.zarr") |
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349 |
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assert reader.shape == (10, 10) |
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assert reader.dtype == np.uint8 |
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352 |
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353 |
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def test_tiff_get_tile(samples_path): |
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355 |
"""Test getting a tile from a TIFF.""" |
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356 |
reader = readers.TIFFReader(samples_path / "CMU-1-Small-Region.svs") |
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357 |
tile = reader.get_tile((1, 1), decode=False) |
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358 |
assert isinstance(tile, bytes) |
|
|
359 |
|
|
|
360 |
|
|
|
361 |
def test_transcode_jpeg_svs_to_zarr(samples_path, tmp_path): |
|
|
362 |
"""Test that we can transcode an JPEG SVS to a Zarr.""" |
|
|
363 |
reader = readers.TIFFReader(samples_path / "CMU-1-Small-Region.svs") |
|
|
364 |
writer = writers.ZarrWriter( |
|
|
365 |
path=tmp_path / "CMU-1-Small-Region.zarr", |
|
|
366 |
shape=reader.shape, |
|
|
367 |
tile_size=reader.tile_shape[::-1], |
|
|
368 |
dtype=reader.dtype, |
|
|
369 |
) |
|
|
370 |
writer.transcode_from_reader(reader=reader) |
|
|
371 |
|
|
|
372 |
assert writer.path.exists() |
|
|
373 |
assert writer.path.is_dir() |
|
|
374 |
assert list(writer.path.iterdir()) |
|
|
375 |
|
|
|
376 |
output = zarr.open(writer.path) |
|
|
377 |
assert np.all(reader[...] == output[0][...]) |
|
|
378 |
|
|
|
379 |
|
|
|
380 |
def test_transcode_svs_to_zarr(samples_path, tmp_path): |
|
|
381 |
"""Test that we can transcode an J2K SVS to a Zarr.""" |
|
|
382 |
reader = readers.Reader.from_file( |
|
|
383 |
samples_path |
|
|
384 |
/ "bfconvert" |
|
|
385 |
/ ( |
|
|
386 |
"XYC_-compression_JPEG-2000" |
|
|
387 |
"_-tilex_128_-tiley_128" |
|
|
388 |
"_-pyramid-scale_2" |
|
|
389 |
"_-merge.ome.tiff" |
|
|
390 |
) |
|
|
391 |
) |
|
|
392 |
writer = writers.ZarrWriter( |
|
|
393 |
path=tmp_path |
|
|
394 |
/ ( |
|
|
395 |
"XYC_-compression_JPEG-2000" |
|
|
396 |
"_-tilex_128_-tiley_128_" |
|
|
397 |
"-pyramid-scale_2_" |
|
|
398 |
"-merge.zarr" |
|
|
399 |
), |
|
|
400 |
shape=reader.shape, |
|
|
401 |
tile_size=reader.tile_shape[::-1], |
|
|
402 |
dtype=reader.dtype, |
|
|
403 |
) |
|
|
404 |
writer.transcode_from_reader(reader=reader) |
|
|
405 |
|
|
|
406 |
assert writer.path.exists() |
|
|
407 |
assert writer.path.is_dir() |
|
|
408 |
assert list(writer.path.iterdir()) |
|
|
409 |
|
|
|
410 |
output = zarr.open(writer.path) |
|
|
411 |
original = reader[...] |
|
|
412 |
new = output[0][...] |
|
|
413 |
|
|
|
414 |
assert np.array_equal(original, new) |
|
|
415 |
|
|
|
416 |
|
|
|
417 |
def test_transcode_svs_to_pyramid_ome_zarr(samples_path, tmp_path): |
|
|
418 |
"""Test that we can transcode an J2K SVS to a pyramid OME-Zarr (NGFF).""" |
|
|
419 |
reader = readers.Reader.from_file( |
|
|
420 |
samples_path |
|
|
421 |
/ "bfconvert" |
|
|
422 |
/ ( |
|
|
423 |
"XYC_-compression_JPEG-2000" |
|
|
424 |
"_-tilex_128_-tiley_128" |
|
|
425 |
"_-pyramid-scale_2" |
|
|
426 |
"_-merge.ome.tiff" |
|
|
427 |
) |
|
|
428 |
) |
|
|
429 |
out_path = tmp_path / ( |
|
|
430 |
"XYC_-compression_JPEG-2000" |
|
|
431 |
"_-tilex_128_-tiley_128_" |
|
|
432 |
"-pyramid-scale_2_" |
|
|
433 |
"-merge.zarr" |
|
|
434 |
) |
|
|
435 |
writer = writers.ZarrWriter( |
|
|
436 |
path=out_path, |
|
|
437 |
shape=reader.shape, |
|
|
438 |
tile_size=reader.tile_shape[::-1], |
|
|
439 |
dtype=reader.dtype, |
|
|
440 |
pyramid_downsamples=[2, 4, 8], |
|
|
441 |
ome=True, |
|
|
442 |
) |
|
|
443 |
writer.transcode_from_reader(reader=reader) |
|
|
444 |
|
|
|
445 |
assert writer.path.exists() |
|
|
446 |
assert writer.path.is_dir() |
|
|
447 |
assert list(writer.path.iterdir()) |
|
|
448 |
|
|
|
449 |
output = zarr.open(writer.path) |
|
|
450 |
original = reader[...] |
|
|
451 |
new = output[0][...] |
|
|
452 |
|
|
|
453 |
assert np.array_equal(original, new) |
|
|
454 |
|
|
|
455 |
assert "_creator" in writer.zarr.attrs |
|
|
456 |
assert "omero" in writer.zarr.attrs |
|
|
457 |
assert "multiscales" in writer.zarr.attrs |
|
|
458 |
|
|
|
459 |
|
|
|
460 |
def test_transcode_jpeg_dicom_wsi_to_zarr(samples_path, tmp_path): |
|
|
461 |
"""Test that we can transcode a JPEG compressed DICOM WSI to a Zarr.""" |
|
|
462 |
reader = readers.DICOMWSIReader(samples_path / "CMU-1-Small-Region") |
|
|
463 |
writer = writers.ZarrWriter( |
|
|
464 |
path=tmp_path / "CMU-1.zarr", |
|
|
465 |
shape=reader.shape, |
|
|
466 |
tile_size=reader.tile_shape[::-1], |
|
|
467 |
dtype=reader.dtype, |
|
|
468 |
) |
|
|
469 |
writer.transcode_from_reader(reader=reader) |
|
|
470 |
|
|
|
471 |
assert writer.path.exists() |
|
|
472 |
assert writer.path.is_dir() |
|
|
473 |
assert list(writer.path.iterdir()) |
|
|
474 |
|
|
|
475 |
output = zarr.open(writer.path) |
|
|
476 |
original = reader[...] |
|
|
477 |
new = output[0][...] |
|
|
478 |
|
|
|
479 |
assert original.shape == new.shape |
|
|
480 |
|
|
|
481 |
# Allow for some slight differences in the pixel values due to |
|
|
482 |
# different decoders. |
|
|
483 |
difference = original.astype(np.float16) - new.astype(np.float16) |
|
|
484 |
mse = (difference**2).mean() |
|
|
485 |
|
|
|
486 |
assert mse < 1.5 |
|
|
487 |
assert np.percentile(np.abs(difference), 95) < 1 |
|
|
488 |
|
|
|
489 |
|
|
|
490 |
def test_transcode_j2k_dicom_wsi_to_zarr(samples_path, tmp_path): |
|
|
491 |
"""Test that we can transcode a J2K compressed DICOM WSI to a Zarr.""" |
|
|
492 |
reader = readers.Reader.from_file(samples_path / "CMU-1-Small-Region-J2K") |
|
|
493 |
writer = writers.ZarrWriter( |
|
|
494 |
path=tmp_path / "CMU-1.zarr", |
|
|
495 |
shape=reader.shape, |
|
|
496 |
tile_size=reader.tile_shape[::-1], |
|
|
497 |
dtype=reader.dtype, |
|
|
498 |
) |
|
|
499 |
writer.transcode_from_reader(reader=reader) |
|
|
500 |
|
|
|
501 |
assert writer.path.exists() |
|
|
502 |
assert writer.path.is_dir() |
|
|
503 |
assert list(writer.path.iterdir()) |
|
|
504 |
|
|
|
505 |
output = zarr.open(writer.path) |
|
|
506 |
original = reader[...] |
|
|
507 |
new = output[0][...] |
|
|
508 |
|
|
|
509 |
assert original.shape == new.shape |
|
|
510 |
|
|
|
511 |
# Allow for some slight differences in the pixel values due to |
|
|
512 |
# different decoders. |
|
|
513 |
difference = original.astype(np.float16) - new.astype(np.float16) |
|
|
514 |
mse = (difference**2).mean() |
|
|
515 |
|
|
|
516 |
assert mse < 1.5 |
|
|
517 |
assert np.percentile(np.abs(difference), 95) < 1 |
|
|
518 |
|
|
|
519 |
|
|
|
520 |
def test_tiff_res_tags(samples_path): |
|
|
521 |
"""Test that we can read the resolution tags from a TIFF.""" |
|
|
522 |
reader = readers.Reader.from_file(samples_path / "XYC-half-mpp.tiff") |
|
|
523 |
assert reader.microns_per_pixel == (0.5, 0.5) |
|
|
524 |
|
|
|
525 |
|
|
|
526 |
def test_copy_from_reader_timeout(samples_path, tmp_path): |
|
|
527 |
"""Check that Writer.copy_from_reader raises IOError when timed out.""" |
|
|
528 |
reader = readers.TIFFReader(samples_path / "CMU-1-Small-Region.svs") |
|
|
529 |
writer = writers.ZarrWriter( |
|
|
530 |
path=tmp_path / "CMU-1-Small-Region.zarr", |
|
|
531 |
shape=reader.shape, |
|
|
532 |
tile_size=reader.tile_shape[::-1], |
|
|
533 |
dtype=reader.dtype, |
|
|
534 |
) |
|
|
535 |
warnings.simplefilter("ignore") |
|
|
536 |
with pytest.raises(IOError, match="timed out"): |
|
|
537 |
writer.copy_from_reader(reader=reader, timeout=0) |
|
|
538 |
|
|
|
539 |
|
|
|
540 |
def test_block_downsample_shape(): |
|
|
541 |
"""Test that the block downsample shape is correct.""" |
|
|
542 |
shape = (135, 145) |
|
|
543 |
block_shape = (32, 32) |
|
|
544 |
downsample = 3 |
|
|
545 |
# (32, 32) / 3 = (10, 10) |
|
|
546 |
# (135, 145) / 32 = (4.21875, 4.53125) |
|
|
547 |
# floor((0.21875, 0.53125) * 10) = (2, 5) |
|
|
548 |
# ((4, 4) * 10) + (2, 5) = (42, 45) |
|
|
549 |
expected = (42, 45) |
|
|
550 |
result_shape, result_tile_shape = utils.block_downsample_shape( |
|
|
551 |
shape=shape, block_shape=block_shape, downsample=downsample |
|
|
552 |
) |
|
|
553 |
assert result_shape == expected |
|
|
554 |
assert result_tile_shape == (10, 10) |
|
|
555 |
|
|
|
556 |
|
|
|
557 |
def test_thumbnail(samples_path): |
|
|
558 |
"""Test generating a thumbnail from a reader.""" |
|
|
559 |
# Compare with cv2 downsampling |
|
|
560 |
reader = readers.TIFFReader(samples_path / "XYC-half-mpp.tiff") |
|
|
561 |
thumbnail = reader.thumbnail(shape=(64, 64)) |
|
|
562 |
cv2_thumbnail = _cv2.resize(reader[...], (64, 64), interpolation=_cv2.INTER_AREA) |
|
|
563 |
assert thumbnail.shape == (64, 64, 3) |
|
|
564 |
assert np.allclose(thumbnail, cv2_thumbnail, atol=1) |
|
|
565 |
|
|
|
566 |
|
|
|
567 |
def test_thumbnail_pil(samples_path, monkeypatch): |
|
|
568 |
"""Test generating a thumbnail from a reader without cv2 installed. |
|
|
569 |
|
|
|
570 |
This should fall back to Pillow. |
|
|
571 |
""" |
|
|
572 |
from PIL import Image |
|
|
573 |
|
|
|
574 |
# Monkeypatch cv2 to not be installed |
|
|
575 |
monkeypatch.setitem(sys.modules, "cv2", None) |
|
|
576 |
|
|
|
577 |
# Sanity check that cv2 is not installed |
|
|
578 |
with pytest.raises(ImportError): |
|
|
579 |
import cv2 # noqa # skipcq |
|
|
580 |
|
|
|
581 |
reader = readers.TIFFReader(samples_path / "XYC-half-mpp.tiff") |
|
|
582 |
thumbnail = reader.thumbnail(shape=(64, 64)) |
|
|
583 |
pil_thumbnail = Image.fromarray(reader[...]).resize( |
|
|
584 |
(64, 64), |
|
|
585 |
resample=Image.Resampling.BOX, |
|
|
586 |
) |
|
|
587 |
assert thumbnail.shape == (64, 64, 3) |
|
|
588 |
|
|
|
589 |
mse = np.mean((thumbnail - pil_thumbnail) ** 2) |
|
|
590 |
assert mse < 1 |
|
|
591 |
assert np.allclose(thumbnail, pil_thumbnail, atol=1) |
|
|
592 |
|
|
|
593 |
|
|
|
594 |
def test_thumbnail_no_cv2_no_pil(samples_path, monkeypatch): |
|
|
595 |
"""Test generating a thumbnail from a reader without cv2 or Pillow installed. |
|
|
596 |
|
|
|
597 |
This should fall back to scipy.ndimage.zoom. |
|
|
598 |
""" |
|
|
599 |
# Monkeypatch cv2 and Pillow to not be installed |
|
|
600 |
monkeypatch.setitem(sys.modules, "cv2", None) |
|
|
601 |
monkeypatch.setitem(sys.modules, "PIL", None) |
|
|
602 |
|
|
|
603 |
# Sanity check that cv2 and Pillow are not installed |
|
|
604 |
with pytest.raises(ImportError): |
|
|
605 |
import cv2 # noqa # skipcq |
|
|
606 |
with pytest.raises(ImportError): |
|
|
607 |
import PIL # noqa # skipcq |
|
|
608 |
|
|
|
609 |
reader = readers.TIFFReader(samples_path / "XYC-half-mpp.tiff") |
|
|
610 |
thumbnail = reader.thumbnail(shape=(64, 64)) |
|
|
611 |
zoom = np.divide((64, 64), reader.shape[:2]) |
|
|
612 |
zoom = np.append(zoom, 1) |
|
|
613 |
cv2_thumbnail = _cv2.resize(reader[...], (64, 64), interpolation=_cv2.INTER_AREA) |
|
|
614 |
assert thumbnail.shape == (64, 64, 3) |
|
|
615 |
assert np.allclose(thumbnail, cv2_thumbnail, atol=1) |
|
|
616 |
|
|
|
617 |
|
|
|
618 |
def test_thumbnail_no_cv2_no_pil_no_scipy(samples_path, monkeypatch): |
|
|
619 |
"""Test generating a thumbnail with nearest neighbor subsampling. |
|
|
620 |
|
|
|
621 |
This should be the raw numpy fallaback. |
|
|
622 |
""" |
|
|
623 |
# Monkeypatch cv2 and Pillow to not be installed |
|
|
624 |
monkeypatch.setitem(sys.modules, "cv2", None) |
|
|
625 |
monkeypatch.setitem(sys.modules, "PIL", None) |
|
|
626 |
monkeypatch.setitem(sys.modules, "scipy", None) |
|
|
627 |
|
|
|
628 |
# Sanity check that modules are not installed |
|
|
629 |
with pytest.raises(ImportError): |
|
|
630 |
import cv2 # noqa # skipcq |
|
|
631 |
with pytest.raises(ImportError): |
|
|
632 |
import PIL # noqa # skipcq |
|
|
633 |
with pytest.raises(ImportError): |
|
|
634 |
import scipy # noqa # skipcq |
|
|
635 |
|
|
|
636 |
reader = readers.TIFFReader(samples_path / "XYC-half-mpp.tiff") |
|
|
637 |
with pytest.warns(UserWarning, match="slower"): |
|
|
638 |
thumbnail = reader.thumbnail(shape=(64, 64)) |
|
|
639 |
cv2_thumbnail = _cv2.resize(reader[...], (64, 64), interpolation=_cv2.INTER_AREA) |
|
|
640 |
assert thumbnail.shape == (64, 64, 3) |
|
|
641 |
assert np.allclose(thumbnail, cv2_thumbnail, atol=1) |
|
|
642 |
|
|
|
643 |
|
|
|
644 |
def test_thumbnail_non_power_two(samples_path): |
|
|
645 |
"""Test generating a thumbnail from a reader. |
|
|
646 |
|
|
|
647 |
Outputs a non power of two sized thumbnail. |
|
|
648 |
""" |
|
|
649 |
# Compare with cv2 downsampling |
|
|
650 |
reader = readers.TIFFReader(samples_path / "CMU-1-Small-Region.svs") |
|
|
651 |
thumbnail = reader.thumbnail(shape=(59, 59)) |
|
|
652 |
cv2_thumbnail = _cv2.resize(reader[...], (59, 59), interpolation=_cv2.INTER_AREA) |
|
|
653 |
assert thumbnail.shape == (59, 59, 3) |
|
|
654 |
mse = np.mean((thumbnail - cv2_thumbnail) ** 2) |
|
|
655 |
psnr = 10 * np.log10(255**2 / mse) |
|
|
656 |
assert psnr > 30 |
|
|
657 |
|
|
|
658 |
|
|
|
659 |
def test_write_rgb_jpeg_svs(samples_path, tmp_path): |
|
|
660 |
"""Test writing an SVS file with RGB JPEG compression.""" |
|
|
661 |
reader = readers.TIFFReader(samples_path / "CMU-1-Small-Region.svs") |
|
|
662 |
writer = writers.SVSWriter( |
|
|
663 |
path=tmp_path / "Neo-CMU-1-Small-Region.svs", |
|
|
664 |
shape=reader.shape, |
|
|
665 |
pyramid_downsamples=[2, 4], |
|
|
666 |
compression_level=70, |
|
|
667 |
) |
|
|
668 |
writer.copy_from_reader(reader=reader) |
|
|
669 |
assert writer.path.exists() |
|
|
670 |
assert writer.path.is_file() |
|
|
671 |
|
|
|
672 |
# Pass the tiffile is_svs test |
|
|
673 |
tiff = tifffile.TiffFile(str(writer.path)) |
|
|
674 |
assert tiff.is_svs |
|
|
675 |
|
|
|
676 |
# Read and compare with OpenSlide |
|
|
677 |
import openslide |
|
|
678 |
|
|
|
679 |
with openslide.OpenSlide(str(writer.path)) as slide: |
|
|
680 |
new_svs_region = slide.read_region((0, 0), 0, (1024, 1024)) |
|
|
681 |
with openslide.OpenSlide(str(samples_path / "CMU-1-Small-Region.svs")) as slide: |
|
|
682 |
old_svs_region = slide.read_region((0, 0), 0, (1024, 1024)) |
|
|
683 |
|
|
|
684 |
# Check mean squared error |
|
|
685 |
# There will be some error due to JPEG compression |
|
|
686 |
mse = (np.subtract(new_svs_region, old_svs_region) ** 2).mean() |
|
|
687 |
assert mse < 10 |
|
|
688 |
|
|
|
689 |
|
|
|
690 |
def test_write_ycbcr_jpeg_svs(samples_path, tmp_path): |
|
|
691 |
"""Test writing an SVS file with YCbCr JPEG compression.""" |
|
|
692 |
reader = readers.TIFFReader(samples_path / "CMU-1-Small-Region.svs") |
|
|
693 |
writer = writers.SVSWriter( |
|
|
694 |
path=tmp_path / "Neo-CMU-1-Small-Region.svs", |
|
|
695 |
shape=reader.shape, |
|
|
696 |
pyramid_downsamples=[2, 4], |
|
|
697 |
compression_level=70, |
|
|
698 |
color_mode="YCbCr", |
|
|
699 |
) |
|
|
700 |
writer.copy_from_reader(reader=reader) |
|
|
701 |
assert writer.path.exists() |
|
|
702 |
assert writer.path.is_file() |
|
|
703 |
|
|
|
704 |
# Pass the tiffile is_svs test |
|
|
705 |
tiff = tifffile.TiffFile(str(writer.path)) |
|
|
706 |
assert tiff.is_svs |
|
|
707 |
|
|
|
708 |
# Read and compare with OpenSlide |
|
|
709 |
import openslide |
|
|
710 |
|
|
|
711 |
with openslide.OpenSlide(str(writer.path)) as slide: |
|
|
712 |
new_svs_region = slide.read_region((0, 0), 0, (1024, 1024)) |
|
|
713 |
with openslide.OpenSlide(str(samples_path / "CMU-1-Small-Region.svs")) as slide: |
|
|
714 |
old_svs_region = slide.read_region((0, 0), 0, (1024, 1024)) |
|
|
715 |
|
|
|
716 |
# Check mean squared error |
|
|
717 |
mse = (np.subtract(new_svs_region, old_svs_region) ** 2).mean() |
|
|
718 |
assert mse < 10 |
|
|
719 |
|
|
|
720 |
|
|
|
721 |
def test_write_ycrcb_j2k_svs_fails(samples_path, tmp_path): |
|
|
722 |
"""Test writing an SVS file with YCrCb JP2 compression fails.""" |
|
|
723 |
reader = readers.TIFFReader(samples_path / "CMU-1-Small-Region.svs") |
|
|
724 |
with pytest.raises(ValueError, match="only supports JPEG"): |
|
|
725 |
writers.SVSWriter( |
|
|
726 |
path=tmp_path / "Neo-CMU-1-Small-Region.svs", |
|
|
727 |
shape=reader.shape, |
|
|
728 |
pyramid_downsamples=[2, 4], |
|
|
729 |
codec=Codec.JPEG2000, |
|
|
730 |
compression_level=70, |
|
|
731 |
photometric=ColorSpace.YCBCR, |
|
|
732 |
) |
|
|
733 |
|
|
|
734 |
|
|
|
735 |
def test_write_jp2_resolution(samples_path, tmp_path): |
|
|
736 |
"""Test writing a JP2 with capture resolution metadata.""" |
|
|
737 |
reader = readers.TIFFReader(samples_path / "CMU-1-Small-Region.svs") |
|
|
738 |
out_path = tmp_path / "CMU-1-Small-Region.jp2" |
|
|
739 |
writer = writers.JP2Writer( |
|
|
740 |
path=out_path, |
|
|
741 |
shape=reader.shape, |
|
|
742 |
pyramid_downsamples=[2, 4, 8], |
|
|
743 |
compression_level=70, |
|
|
744 |
microns_per_pixel=(0.5, 0.5), |
|
|
745 |
) |
|
|
746 |
writer.copy_from_reader(reader=reader) |
|
|
747 |
jp2_reader = readers.JP2Reader(out_path) |
|
|
748 |
assert jp2_reader.microns_per_pixel == (0.5, 0.5) |
|
|
749 |
|
|
|
750 |
|
|
|
751 |
def test_missing_imagecodecs_codec(samples_path, tmp_path): |
|
|
752 |
"""Test writing an SVS file with YCrCb JP2 compression fails.""" |
|
|
753 |
reader = readers.TIFFReader(samples_path / "CMU-1-Small-Region.svs") |
|
|
754 |
with pytest.raises(ValueError, match="Unknown"): |
|
|
755 |
writers.ZarrWriter( |
|
|
756 |
path=tmp_path / "test.zarr", |
|
|
757 |
shape=reader.shape, |
|
|
758 |
pyramid_downsamples=[2, 4], |
|
|
759 |
codec="foo", |
|
|
760 |
compression_level=70, |
|
|
761 |
color_space=ColorSpace.RGB, |
|
|
762 |
) |
|
|
763 |
|
|
|
764 |
|
|
|
765 |
# Zarr tests for alternate stores |
|
|
766 |
|
|
|
767 |
|
|
|
768 |
def test_write_read_sqlite_store_zarr(samples_path, tmp_path): |
|
|
769 |
"""Test writing and reading a Zarr with an SQLite store.""" |
|
|
770 |
reader = readers.TIFFReader(samples_path / "CMU-1-Small-Region.svs") |
|
|
771 |
writer = writers.ZarrWriter( |
|
|
772 |
shape=reader.shape, |
|
|
773 |
store=zarr.SQLiteStore(tmp_path / "test.zarr.sqlite"), |
|
|
774 |
) |
|
|
775 |
writer.copy_from_reader(reader=reader) |
|
|
776 |
writer.close() |
|
|
777 |
|
|
|
778 |
# SQLite doesn't have a standard file extension. Therefore it is |
|
|
779 |
# hard to infer that the file is a SQLite file. We must pass a |
|
|
780 |
# zarr.SQLiteStore instance to open it. |
|
|
781 |
readers.ZarrReader(zarr.SQLiteStore(tmp_path / "test.zarr.sqlite")) |
|
|
782 |
|
|
|
783 |
|
|
|
784 |
def test_write_read_zip_store_zarr(samples_path, tmp_path): |
|
|
785 |
"""Test writing and reading a Zarr with a Zip store.""" |
|
|
786 |
reader = readers.TIFFReader(samples_path / "CMU-1-Small-Region.svs") |
|
|
787 |
writer = writers.ZarrWriter( |
|
|
788 |
path=tmp_path / "test.zarr.zip", |
|
|
789 |
shape=reader.shape, |
|
|
790 |
) |
|
|
791 |
|
|
|
792 |
writer.copy_from_reader(reader=reader) |
|
|
793 |
writer.close() # Important for zip store! |
|
|
794 |
|
|
|
795 |
readers.ZarrReader(tmp_path / "test.zarr.zip") |
|
|
796 |
|
|
|
797 |
|
|
|
798 |
def test_write_read_temp_store_zarr(samples_path): |
|
|
799 |
"""Test writing and reading a Zarr with a Temp store.""" |
|
|
800 |
reader = readers.TIFFReader(samples_path / "CMU-1-Small-Region.svs") |
|
|
801 |
writer = writers.ZarrWriter( |
|
|
802 |
shape=reader.shape, |
|
|
803 |
store=zarr.TempStore("test.zarr"), |
|
|
804 |
) |
|
|
805 |
writer.copy_from_reader(reader=reader) |
|
|
806 |
|
|
|
807 |
readers.ZarrReader(writer.zarr.store) |
|
|
808 |
|
|
|
809 |
|
|
|
810 |
# Test Scenarios |
|
|
811 |
|
|
|
812 |
WRITER_EXT_MAPPING = { |
|
|
813 |
".zarr": writers.ZarrWriter, |
|
|
814 |
".tiff": writers.TIFFWriter, |
|
|
815 |
".dcm": writers.DICOMWSIWriter, |
|
|
816 |
} |
|
|
817 |
|
|
|
818 |
|
|
|
819 |
class TestTranscodeScenarios: |
|
|
820 |
"""Test scenarios for the transcoding WSIs.""" |
|
|
821 |
|
|
|
822 |
scenarios = [ |
|
|
823 |
( |
|
|
824 |
"jpeg_svs_to_zarr", |
|
|
825 |
{ |
|
|
826 |
"sample_name": "CMU-1-Small-Region.svs", |
|
|
827 |
"reader_cls": readers.TIFFReader, |
|
|
828 |
"out_reader": readers.ZarrReader, |
|
|
829 |
"out_ext": ".zarr", |
|
|
830 |
}, |
|
|
831 |
), |
|
|
832 |
( |
|
|
833 |
"jpeg_tiff_to_zarr", |
|
|
834 |
{ |
|
|
835 |
"sample_name": "CMU-1-Small-Region.jpeg.tiff", |
|
|
836 |
"reader_cls": readers.TIFFReader, |
|
|
837 |
"out_reader": readers.ZarrReader, |
|
|
838 |
"out_ext": ".zarr", |
|
|
839 |
}, |
|
|
840 |
), |
|
|
841 |
( |
|
|
842 |
"webp_tiff_to_zarr", |
|
|
843 |
{ |
|
|
844 |
"sample_name": "CMU-1-Small-Region.webp.tiff", |
|
|
845 |
"reader_cls": readers.TIFFReader, |
|
|
846 |
"out_reader": readers.ZarrReader, |
|
|
847 |
"out_ext": ".zarr", |
|
|
848 |
}, |
|
|
849 |
), |
|
|
850 |
( |
|
|
851 |
"jp2_tiff_to_zarr", |
|
|
852 |
{ |
|
|
853 |
"sample_name": "CMU-1-Small-Region.jp2.tiff", |
|
|
854 |
"reader_cls": readers.TIFFReader, |
|
|
855 |
"out_reader": readers.ZarrReader, |
|
|
856 |
"out_ext": ".zarr", |
|
|
857 |
}, |
|
|
858 |
), |
|
|
859 |
( |
|
|
860 |
"jpeg_dicom_to_zarr", |
|
|
861 |
{ |
|
|
862 |
"sample_name": "CMU-1-Small-Region", |
|
|
863 |
"reader_cls": readers.DICOMWSIReader, |
|
|
864 |
"out_reader": readers.ZarrReader, |
|
|
865 |
"out_ext": ".zarr", |
|
|
866 |
}, |
|
|
867 |
), |
|
|
868 |
( |
|
|
869 |
"j2k_dicom_to_zarr", |
|
|
870 |
{ |
|
|
871 |
"sample_name": "CMU-1-Small-Region-J2K", |
|
|
872 |
"reader_cls": readers.DICOMWSIReader, |
|
|
873 |
"out_reader": readers.ZarrReader, |
|
|
874 |
"out_ext": ".zarr", |
|
|
875 |
}, |
|
|
876 |
), |
|
|
877 |
( |
|
|
878 |
"jpeg_dicom_to_tiff", |
|
|
879 |
{ |
|
|
880 |
"sample_name": "CMU-1-Small-Region", |
|
|
881 |
"reader_cls": readers.DICOMWSIReader, |
|
|
882 |
"out_reader": readers.TIFFReader, |
|
|
883 |
"out_ext": ".tiff", |
|
|
884 |
}, |
|
|
885 |
), |
|
|
886 |
( |
|
|
887 |
"j2k_dicom_to_tiff", |
|
|
888 |
{ |
|
|
889 |
"sample_name": "CMU-1-Small-Region-J2K", |
|
|
890 |
"reader_cls": readers.DICOMWSIReader, |
|
|
891 |
"out_reader": readers.TIFFReader, |
|
|
892 |
"out_ext": ".tiff", |
|
|
893 |
}, |
|
|
894 |
), |
|
|
895 |
( |
|
|
896 |
"webp_tiff_to_tiff", |
|
|
897 |
{ |
|
|
898 |
"sample_name": "CMU-1-Small-Region.webp.tiff", |
|
|
899 |
"reader_cls": readers.TIFFReader, |
|
|
900 |
"out_reader": readers.TIFFReader, |
|
|
901 |
"out_ext": ".tiff", |
|
|
902 |
}, |
|
|
903 |
), |
|
|
904 |
( |
|
|
905 |
"jpeg_tiff_to_jpeg_dicom", |
|
|
906 |
{ |
|
|
907 |
"sample_name": "CMU-1-Small-Region.jpeg.tiff", |
|
|
908 |
"reader_cls": readers.TIFFReader, |
|
|
909 |
"out_reader": readers.DICOMWSIReader, |
|
|
910 |
"out_ext": ".dcm", |
|
|
911 |
}, |
|
|
912 |
), |
|
|
913 |
] |
|
|
914 |
|
|
|
915 |
@staticmethod |
|
|
916 |
def test_transcode_tiled( |
|
|
917 |
samples_path: Path, |
|
|
918 |
sample_name: str, |
|
|
919 |
reader_cls: readers.Reader, |
|
|
920 |
out_reader: readers.Reader, |
|
|
921 |
out_ext: str, |
|
|
922 |
tmp_path: Path, |
|
|
923 |
): |
|
|
924 |
"""Test transcoding a tiled WSI.""" |
|
|
925 |
in_path = samples_path / sample_name |
|
|
926 |
out_path = (tmp_path / sample_name).with_suffix(out_ext) |
|
|
927 |
reader: Reader = reader_cls(in_path) |
|
|
928 |
writer_cls = WRITER_EXT_MAPPING[out_ext] |
|
|
929 |
writer: Writer = writer_cls( |
|
|
930 |
path=out_path, |
|
|
931 |
shape=reader.shape, |
|
|
932 |
tile_size=reader.tile_shape[::-1], |
|
|
933 |
) |
|
|
934 |
writer.transcode_from_reader(reader=reader) |
|
|
935 |
output_reader: Reader = out_reader(out_path) |
|
|
936 |
|
|
|
937 |
assert output_reader.shape == reader.shape |
|
|
938 |
assert output_reader.tile_shape == reader.tile_shape |
|
|
939 |
|
|
|
940 |
# Calculate error metrics |
|
|
941 |
reader_img = reader[...] |
|
|
942 |
output_reader_img = output_reader[...] |
|
|
943 |
squared_err = np.subtract(reader_img, output_reader_img) ** 2 |
|
|
944 |
abs_err = np.abs(np.subtract(reader_img, output_reader_img)) |
|
|
945 |
|
|
|
946 |
# A lot of the image should have zero error |
|
|
947 |
assert np.count_nonzero(abs_err) / abs_err.size < 0.25 |
|
|
948 |
|
|
|
949 |
# Check mean squared error is low |
|
|
950 |
assert np.all(squared_err.mean() < 2.56) |
|
|
951 |
|
|
|
952 |
# Check mean absolute error is low |
|
|
953 |
assert np.all(abs_err.mean() < 25.6) |
|
|
954 |
|
|
|
955 |
def visually_compare_readers( |
|
|
956 |
self, |
|
|
957 |
in_path: Path, |
|
|
958 |
out_path: Path, |
|
|
959 |
reader: readers.Reader, |
|
|
960 |
output_reader: readers.Reader, |
|
|
961 |
) -> Dict[str, bool]: |
|
|
962 |
"""Compare two readers for manual visual inspection. |
|
|
963 |
|
|
|
964 |
Used for debugging. |
|
|
965 |
|
|
|
966 |
Args: |
|
|
967 |
in_path: |
|
|
968 |
Path to the input file. |
|
|
969 |
out_path: |
|
|
970 |
Path to the output file. |
|
|
971 |
reader: |
|
|
972 |
Reader for the input file. |
|
|
973 |
output_reader: |
|
|
974 |
Reader for the output file. |
|
|
975 |
""" |
|
|
976 |
import inspect |
|
|
977 |
|
|
|
978 |
from matplotlib import pyplot as plt # type: ignore |
|
|
979 |
from matplotlib.widgets import Button # type: ignore |
|
|
980 |
|
|
|
981 |
current_frame = inspect.currentframe() |
|
|
982 |
class_name = self.__class__.__name__ |
|
|
983 |
function_name = current_frame.f_back.f_code.co_name |
|
|
984 |
# Create a dictionary of arg names to values |
|
|
985 |
args, _, _, values = inspect.getargvalues(current_frame) |
|
|
986 |
args_dict = {arg: values[arg] for arg in args} |
|
|
987 |
function_arguments = ",\n ".join( |
|
|
988 |
f"{k}={v}" if k not in ("self",) else k for k, v in args_dict.items() |
|
|
989 |
) |
|
|
990 |
|
|
|
991 |
# Display the function signature and arguments in axs[0] |
|
|
992 |
text_figure = plt.gcf() |
|
|
993 |
text_figure.canvas.set_window_title(f"{class_name} - {function_name}") |
|
|
994 |
text_figure.set_size_inches(8, 2) |
|
|
995 |
plt.suptitle( |
|
|
996 |
f"{function_name}(\n {function_arguments}\n)", |
|
|
997 |
horizontalalignment="left", |
|
|
998 |
verticalalignment="top", |
|
|
999 |
x=0, |
|
|
1000 |
) |
|
|
1001 |
plt.show(block=False) |
|
|
1002 |
|
|
|
1003 |
# Plot the readers to compare |
|
|
1004 |
_, axs = plt.subplots(1, 3, sharex=True, sharey=True) |
|
|
1005 |
axs[0].imshow(reader[...]) |
|
|
1006 |
axs[0].set_title(f"Input\n({in_path.name})") |
|
|
1007 |
axs[1].imshow(output_reader[...]) |
|
|
1008 |
axs[1].set_title(f"Output\n({out_path.name})") |
|
|
1009 |
diff = np.abs(np.subtract(reader[...], output_reader[...], dtype=float)) |
|
|
1010 |
axs[2].imshow(diff.mean(-1)) |
|
|
1011 |
max_diff = diff.max(axis=(0, 1)) |
|
|
1012 |
mean_diff = diff.mean(axis=(0, 1)) |
|
|
1013 |
axs[2].set_title( |
|
|
1014 |
f"Difference\nChannel Max Diff {max_diff}\nChannel Mean Diff {mean_diff}" |
|
|
1015 |
) |
|
|
1016 |
|
|
|
1017 |
# Set the window title |
|
|
1018 |
plt.gcf().canvas.set_window_title(f"{class_name} - {function_name}") |
|
|
1019 |
|
|
|
1020 |
# Add Pass / Fail Buttons with function callbacks |
|
|
1021 |
visual_inspections_passed = {} |
|
|
1022 |
|
|
|
1023 |
def pass_callback(event): |
|
|
1024 |
"""Callback for the pass button.""" |
|
|
1025 |
visual_inspections_passed[function_name] = True |
|
|
1026 |
plt.close(text_figure) |
|
|
1027 |
plt.close() |
|
|
1028 |
|
|
|
1029 |
def fail_callback(event): |
|
|
1030 |
"""Callback for the fail button.""" |
|
|
1031 |
plt.close(text_figure) |
|
|
1032 |
plt.close() |
|
|
1033 |
|
|
|
1034 |
ax_pass = plt.axes([0.8, 0.05, 0.1, 0.075]) |
|
|
1035 |
btn_pass = Button(ax_pass, "Pass", color="lightgreen") |
|
|
1036 |
btn_pass.on_clicked(pass_callback) |
|
|
1037 |
ax_fail = plt.axes([0.9, 0.05, 0.1, 0.075]) |
|
|
1038 |
btn_fail = Button(ax_fail, "Fail", color="red") |
|
|
1039 |
btn_fail.on_clicked(fail_callback) |
|
|
1040 |
|
|
|
1041 |
# Set suptitle to the function name |
|
|
1042 |
plt.suptitle("\n".join([class_name, function_name])) |
|
|
1043 |
plt.tight_layout() |
|
|
1044 |
plt.show(block=True) |
|
|
1045 |
|
|
|
1046 |
return visual_inspections_passed # noqa: R504 |
|
|
1047 |
|
|
|
1048 |
|
|
|
1049 |
class TestConvertScenarios: |
|
|
1050 |
"""Test scenarios for converting between formats.""" |
|
|
1051 |
|
|
|
1052 |
scenarios = [ |
|
|
1053 |
( |
|
|
1054 |
"j2k_dicom_to_zarr", |
|
|
1055 |
{ |
|
|
1056 |
"sample_name": "CMU-1-Small-Region-J2K", |
|
|
1057 |
"reader_cls": readers.DICOMWSIReader, |
|
|
1058 |
"writer_cls": writers.ZarrWriter, |
|
|
1059 |
"out_ext": ".zarr", |
|
|
1060 |
"codec": "blosc", |
|
|
1061 |
}, |
|
|
1062 |
), |
|
|
1063 |
( |
|
|
1064 |
"jpeg_dicom_to_blosc_zarr", |
|
|
1065 |
{ |
|
|
1066 |
"sample_name": "CMU-1-Small-Region", |
|
|
1067 |
"reader_cls": readers.DICOMWSIReader, |
|
|
1068 |
"writer_cls": writers.ZarrWriter, |
|
|
1069 |
"out_ext": ".zarr", |
|
|
1070 |
"codec": "blosc", |
|
|
1071 |
}, |
|
|
1072 |
), |
|
|
1073 |
( |
|
|
1074 |
"jpeg_dicom_to_jpeg_zarr", |
|
|
1075 |
{ |
|
|
1076 |
"sample_name": "CMU-1-Small-Region", |
|
|
1077 |
"reader_cls": readers.DICOMWSIReader, |
|
|
1078 |
"writer_cls": writers.ZarrWriter, |
|
|
1079 |
"out_ext": ".zarr", |
|
|
1080 |
"codec": "jpeg", |
|
|
1081 |
}, |
|
|
1082 |
), |
|
|
1083 |
( |
|
|
1084 |
"jp2_to_jpeg_tiff", |
|
|
1085 |
{ |
|
|
1086 |
"sample_name": "XYC.jp2", |
|
|
1087 |
"reader_cls": readers.JP2Reader, |
|
|
1088 |
"writer_cls": writers.TIFFWriter, |
|
|
1089 |
"out_ext": ".tiff", |
|
|
1090 |
"codec": "jpeg", |
|
|
1091 |
}, |
|
|
1092 |
), |
|
|
1093 |
( |
|
|
1094 |
"jp2_to_zarr", |
|
|
1095 |
{ |
|
|
1096 |
"sample_name": "XYC.jp2", |
|
|
1097 |
"reader_cls": readers.JP2Reader, |
|
|
1098 |
"writer_cls": writers.ZarrWriter, |
|
|
1099 |
"out_ext": ".zarr", |
|
|
1100 |
"codec": "blosc", |
|
|
1101 |
}, |
|
|
1102 |
), |
|
|
1103 |
( |
|
|
1104 |
"jp2_to_jpeg_svs", |
|
|
1105 |
{ |
|
|
1106 |
"sample_name": "XYC.jp2", |
|
|
1107 |
"reader_cls": readers.JP2Reader, |
|
|
1108 |
"writer_cls": writers.SVSWriter, |
|
|
1109 |
"out_ext": ".svs", |
|
|
1110 |
"codec": "jpeg", |
|
|
1111 |
}, |
|
|
1112 |
), |
|
|
1113 |
( |
|
|
1114 |
"tiff_to_jp2", |
|
|
1115 |
{ |
|
|
1116 |
"sample_name": "XYC-half-mpp.tiff", |
|
|
1117 |
"reader_cls": readers.TIFFReader, |
|
|
1118 |
"writer_cls": writers.JP2Writer, |
|
|
1119 |
"out_ext": ".jp2", |
|
|
1120 |
"codec": "jpeg2000", |
|
|
1121 |
}, |
|
|
1122 |
), |
|
|
1123 |
( |
|
|
1124 |
"jp2_to_zstd_tiff", |
|
|
1125 |
{ |
|
|
1126 |
"sample_name": "XYC.jp2", |
|
|
1127 |
"reader_cls": readers.JP2Reader, |
|
|
1128 |
"writer_cls": writers.TIFFWriter, |
|
|
1129 |
"out_ext": ".tiff", |
|
|
1130 |
"codec": "zstd", |
|
|
1131 |
}, |
|
|
1132 |
), |
|
|
1133 |
( |
|
|
1134 |
"jp2_to_png_tiff", |
|
|
1135 |
{ |
|
|
1136 |
"sample_name": "XYC.jp2", |
|
|
1137 |
"reader_cls": readers.JP2Reader, |
|
|
1138 |
"writer_cls": writers.TIFFWriter, |
|
|
1139 |
"out_ext": ".tiff", |
|
|
1140 |
"codec": "png", |
|
|
1141 |
}, |
|
|
1142 |
), |
|
|
1143 |
( |
|
|
1144 |
"jp2_to_jpegxr_tiff", |
|
|
1145 |
{ |
|
|
1146 |
"sample_name": "XYC.jp2", |
|
|
1147 |
"reader_cls": readers.JP2Reader, |
|
|
1148 |
"writer_cls": writers.TIFFWriter, |
|
|
1149 |
"out_ext": ".tiff", |
|
|
1150 |
"codec": "jpegxr", |
|
|
1151 |
}, |
|
|
1152 |
), |
|
|
1153 |
( |
|
|
1154 |
"jp2_to_deflate_tiff", |
|
|
1155 |
{ |
|
|
1156 |
"sample_name": "XYC.jp2", |
|
|
1157 |
"reader_cls": readers.JP2Reader, |
|
|
1158 |
"writer_cls": writers.TIFFWriter, |
|
|
1159 |
"out_ext": ".tiff", |
|
|
1160 |
"codec": "deflate", |
|
|
1161 |
}, |
|
|
1162 |
), |
|
|
1163 |
( |
|
|
1164 |
"jp2_to_jpegxl_tiff", |
|
|
1165 |
{ |
|
|
1166 |
"sample_name": "XYC.jp2", |
|
|
1167 |
"reader_cls": readers.JP2Reader, |
|
|
1168 |
"writer_cls": writers.TIFFWriter, |
|
|
1169 |
"out_ext": ".tiff", |
|
|
1170 |
"codec": "jpegxl", |
|
|
1171 |
}, |
|
|
1172 |
), |
|
|
1173 |
( |
|
|
1174 |
"jp2_to_jpeg_dicom", |
|
|
1175 |
{ |
|
|
1176 |
"sample_name": "XYC.jp2", |
|
|
1177 |
"reader_cls": readers.JP2Reader, |
|
|
1178 |
"writer_cls": writers.DICOMWSIWriter, |
|
|
1179 |
"out_ext": ".dcm", |
|
|
1180 |
"codec": "jpeg", |
|
|
1181 |
}, |
|
|
1182 |
), |
|
|
1183 |
( |
|
|
1184 |
"svs_to_jppeg_dicom", |
|
|
1185 |
{ |
|
|
1186 |
"sample_name": "CMU-1-Small-Region.svs", |
|
|
1187 |
"reader_cls": readers.OpenSlideReader, |
|
|
1188 |
"writer_cls": writers.DICOMWSIWriter, |
|
|
1189 |
"out_ext": ".dcm", |
|
|
1190 |
"codec": "jpeg", |
|
|
1191 |
}, |
|
|
1192 |
), |
|
|
1193 |
( |
|
|
1194 |
"tiff_to_dicom", |
|
|
1195 |
{ |
|
|
1196 |
"sample_name": "XYC-half-mpp.tiff", |
|
|
1197 |
"reader_cls": readers.TIFFReader, |
|
|
1198 |
"writer_cls": writers.DICOMWSIWriter, |
|
|
1199 |
"out_ext": ".dcm", |
|
|
1200 |
"codec": "jpeg", |
|
|
1201 |
}, |
|
|
1202 |
), |
|
|
1203 |
( |
|
|
1204 |
"zarr_to_jpeg_dicom", |
|
|
1205 |
{ |
|
|
1206 |
"sample_name": "CMU-1-Small-Region-JPEG.zarr", |
|
|
1207 |
"reader_cls": readers.ZarrReader, |
|
|
1208 |
"writer_cls": writers.DICOMWSIWriter, |
|
|
1209 |
"out_ext": ".tiff", |
|
|
1210 |
"codec": "jpeg", |
|
|
1211 |
}, |
|
|
1212 |
), |
|
|
1213 |
] |
|
|
1214 |
|
|
|
1215 |
@staticmethod |
|
|
1216 |
def test_convert( |
|
|
1217 |
samples_path: Path, |
|
|
1218 |
sample_name: str, |
|
|
1219 |
reader_cls: readers.Reader, |
|
|
1220 |
writer_cls: writers.Writer, |
|
|
1221 |
out_ext: str, |
|
|
1222 |
tmp_path: Path, |
|
|
1223 |
codec: str, |
|
|
1224 |
): |
|
|
1225 |
"""Test converting between formats.""" |
|
|
1226 |
in_path = samples_path / sample_name |
|
|
1227 |
out_path = (tmp_path / sample_name).with_suffix(out_ext) |
|
|
1228 |
reader: Reader = reader_cls(in_path) |
|
|
1229 |
writer: Writer = writer_cls( |
|
|
1230 |
out_path, |
|
|
1231 |
shape=reader.shape, |
|
|
1232 |
codec=codec, |
|
|
1233 |
compression_level=4 |
|
|
1234 |
if codec |
|
|
1235 |
in { |
|
|
1236 |
"blosc", |
|
|
1237 |
} |
|
|
1238 |
else 100, |
|
|
1239 |
) |
|
|
1240 |
writer.copy_from_reader( |
|
|
1241 |
reader, |
|
|
1242 |
num_workers=1, |
|
|
1243 |
timeout=1e32, |
|
|
1244 |
) |
|
|
1245 |
|
|
|
1246 |
# Check that the output file exists |
|
|
1247 |
assert out_path.exists() |
|
|
1248 |
|
|
|
1249 |
# Check that the output file has non-zero size |
|
|
1250 |
assert out_path.stat().st_size > 0 |
|
|
1251 |
|
|
|
1252 |
# Check that the output looks the same as the input |
|
|
1253 |
output_reader = readers.Reader.from_file(out_path) |
|
|
1254 |
mse = np.mean(np.square(reader[...] - output_reader[...])) |
|
|
1255 |
assert mse < 100 |
|
|
1256 |
|
|
|
1257 |
|
|
|
1258 |
class TestWriterScenarios: |
|
|
1259 |
"""Test scenarios for writing to formats with codecs.""" |
|
|
1260 |
|
|
|
1261 |
scenarios = [ |
|
|
1262 |
("svs_jpeg", {"writer_cls": writers.SVSWriter, "codec": "jpeg"}), |
|
|
1263 |
# Unsupported by tifffile |
|
|
1264 |
# ("tiff_blosc", {"writer_cls": writers.TIFFWriter, "codec": "blosc"}), |
|
|
1265 |
# ("tiff_blosc2", {"writer_cls": writers.TIFFWriter, "codec": "blosc2"}), |
|
|
1266 |
# ("tiff_brotli", {"writer_cls": writers.TIFFWriter, "codec": "brotli"}), |
|
|
1267 |
("tiff_deflate", {"writer_cls": writers.TIFFWriter, "codec": "deflate"}), |
|
|
1268 |
# Unsupported by tifffile |
|
|
1269 |
# ("tiff_j2k", {"writer_cls": writers.TIFFWriter, "codec": "j2k"}), |
|
|
1270 |
("tiff_jp2", {"writer_cls": writers.TIFFWriter, "codec": "jpeg2000"}), |
|
|
1271 |
("tiff_jpeg", {"writer_cls": writers.TIFFWriter, "codec": "jpeg"}), |
|
|
1272 |
# Unsupported by tifffile |
|
|
1273 |
# ("tiff_jpls", {"writer_cls": writers.TIFFWriter, "codec": "jpegls"}), |
|
|
1274 |
("tiff_jpxl", {"writer_cls": writers.TIFFWriter, "codec": "jpegxl"}), |
|
|
1275 |
("tiff_jpxr", {"writer_cls": writers.TIFFWriter, "codec": "jpegxr"}), |
|
|
1276 |
# Unsupported by tifffile |
|
|
1277 |
# ("tiff_lz4", {"writer_cls": writers.TIFFWriter, "codec": "lz4"}), |
|
|
1278 |
# Encode unsupported by imagecodecs |
|
|
1279 |
# ("tiff_lzw", {"writer_cls": writers.TIFFWriter, "codec": "lzw"}), |
|
|
1280 |
("tiff_png", {"writer_cls": writers.TIFFWriter, "codec": "png"}), |
|
|
1281 |
("tiff_webp", {"writer_cls": writers.TIFFWriter, "codec": "webp"}), |
|
|
1282 |
# Unsupported by tifffile |
|
|
1283 |
# ("tiff_zfp", {"writer_cls": writers.TIFFWriter, "codec": "zfp"}), |
|
|
1284 |
("tiff_zstd", {"writer_cls": writers.TIFFWriter, "codec": "zstd"}), |
|
|
1285 |
("zarr_blosc", {"writer_cls": writers.ZarrWriter, "codec": "blosc"}), |
|
|
1286 |
("zarr_blosc2", {"writer_cls": writers.ZarrWriter, "codec": "blosc2"}), |
|
|
1287 |
("zarr_brotli", {"writer_cls": writers.ZarrWriter, "codec": "brotli"}), |
|
|
1288 |
("zarr_deflate", {"writer_cls": writers.ZarrWriter, "codec": "deflate"}), |
|
|
1289 |
("zarr_j2k", {"writer_cls": writers.ZarrWriter, "codec": "j2k"}), |
|
|
1290 |
("zarr_jp2", {"writer_cls": writers.ZarrWriter, "codec": "jpeg2000"}), |
|
|
1291 |
("zarr_jpeg", {"writer_cls": writers.ZarrWriter, "codec": "jpeg"}), |
|
|
1292 |
("zarr_jpls", {"writer_cls": writers.ZarrWriter, "codec": "jpegls"}), |
|
|
1293 |
("zarr_jpxl", {"writer_cls": writers.ZarrWriter, "codec": "jpegxl"}), |
|
|
1294 |
("zarr_jpxr", {"writer_cls": writers.ZarrWriter, "codec": "jpegxr"}), |
|
|
1295 |
("zarr_lz4", {"writer_cls": writers.ZarrWriter, "codec": "lz4"}), |
|
|
1296 |
# Encode unsupported by imagecodecs |
|
|
1297 |
# ("zarr_lzw", {"writer_cls": writers.ZarrWriter, "codec": "lzw"}), |
|
|
1298 |
("zarr_png", {"writer_cls": writers.ZarrWriter, "codec": "png"}), |
|
|
1299 |
("zarr_webp", {"writer_cls": writers.ZarrWriter, "codec": "webp"}), |
|
|
1300 |
( |
|
|
1301 |
"zarr_zfp", |
|
|
1302 |
{"writer_cls": writers.ZarrWriter, "codec": "zfp"}, |
|
|
1303 |
), # Wrong data type |
|
|
1304 |
("zarr_zstd", {"writer_cls": writers.ZarrWriter, "codec": "zstd"}), |
|
|
1305 |
] |
|
|
1306 |
|
|
|
1307 |
@staticmethod |
|
|
1308 |
def test_write( |
|
|
1309 |
samples_path: Path, tmp_path: Path, writer_cls: writers.Writer, codec: str |
|
|
1310 |
): |
|
|
1311 |
"""Test writing to a format does not error.""" |
|
|
1312 |
reader = readers.Reader.from_file(samples_path / "CMU-1-Small-Region.svs") |
|
|
1313 |
writer = writer_cls( |
|
|
1314 |
tmp_path / "image", |
|
|
1315 |
shape=reader.shape, |
|
|
1316 |
codec=codec, |
|
|
1317 |
dtype=float if codec == "zfp" else np.uint8, |
|
|
1318 |
) |
|
|
1319 |
writer.copy_from_reader(reader) |
|
|
1320 |
|
|
|
1321 |
|
|
|
1322 |
class TestReaderScenarios: |
|
|
1323 |
"""Test scenarios for readers.""" |
|
|
1324 |
|
|
|
1325 |
scenarios = [ |
|
|
1326 |
( |
|
|
1327 |
"jpeg_svs_tifffile", |
|
|
1328 |
{ |
|
|
1329 |
"sample_name": "CMU-1-Small-Region.svs", |
|
|
1330 |
"reader_cls": readers.TIFFReader, |
|
|
1331 |
}, |
|
|
1332 |
), |
|
|
1333 |
( |
|
|
1334 |
"jpeg_svs_openslide", |
|
|
1335 |
{ |
|
|
1336 |
"sample_name": "CMU-1-Small-Region.svs", |
|
|
1337 |
"reader_cls": readers.OpenSlideReader, |
|
|
1338 |
}, |
|
|
1339 |
), |
|
|
1340 |
( |
|
|
1341 |
"j2k_dicom", |
|
|
1342 |
{ |
|
|
1343 |
"sample_name": "CMU-1-Small-Region-J2K", |
|
|
1344 |
"reader_cls": readers.DICOMWSIReader, |
|
|
1345 |
}, |
|
|
1346 |
), |
|
|
1347 |
( |
|
|
1348 |
"jpeg_dicom", |
|
|
1349 |
{ |
|
|
1350 |
"sample_name": "CMU-1-Small-Region", |
|
|
1351 |
"reader_cls": readers.DICOMWSIReader, |
|
|
1352 |
}, |
|
|
1353 |
), |
|
|
1354 |
( |
|
|
1355 |
"jpeg_zarr", |
|
|
1356 |
{ |
|
|
1357 |
"sample_name": "CMU-1-Small-Region-JPEG.zarr", |
|
|
1358 |
"reader_cls": readers.ZarrReader, |
|
|
1359 |
}, |
|
|
1360 |
), |
|
|
1361 |
] |
|
|
1362 |
|
|
|
1363 |
@staticmethod |
|
|
1364 |
def test_thumbnail_512_512_approx( |
|
|
1365 |
samples_path: Path, |
|
|
1366 |
sample_name: str, |
|
|
1367 |
reader_cls: readers.Reader, |
|
|
1368 |
): |
|
|
1369 |
"""Test creating a thumbnail.""" |
|
|
1370 |
in_path = samples_path / sample_name |
|
|
1371 |
reader: readers.Reader = reader_cls(in_path) |
|
|
1372 |
reader.thumbnail( |
|
|
1373 |
shape=(512, 512), |
|
|
1374 |
approx_ok=True, |
|
|
1375 |
) |
|
|
1376 |
|
|
|
1377 |
@staticmethod |
|
|
1378 |
def test_mpp_found( |
|
|
1379 |
samples_path: Path, |
|
|
1380 |
sample_name: str, |
|
|
1381 |
reader_cls: readers.Reader, |
|
|
1382 |
): |
|
|
1383 |
"""Check that resolution/mpp is read from the file (not None).""" |
|
|
1384 |
in_path = samples_path / sample_name |
|
|
1385 |
reader: readers.Reader = reader_cls(in_path) |
|
|
1386 |
assert hasattr(reader, "microns_per_pixel") |
|
|
1387 |
# A plain zarr doesn't have a resolution attribute so skip this. |
|
|
1388 |
# Maybe in duture this could be done via the xarray metadata. |
|
|
1389 |
if ( |
|
|
1390 |
not isinstance(reader, readers.ZarrReader) |
|
|
1391 |
or sample_name != "CMU-1-Small-Region-JPEG.zarr" |
|
|
1392 |
): |
|
|
1393 |
assert reader.microns_per_pixel is not None |