--- a +++ b/README.md @@ -0,0 +1,39 @@ +Quantitative Histology +====================== + +Context +------- + +Tissue samples can contain significant information on the state of health of an individual, and their +interpretation can aid in the elucidation of mechanisms of degeneration of health. Commonly, a small sample of +images is captured from histological preparations, and these images are assessed by eye. This can be +prohibitatively slow at the population level, and human error can cause bias in the results. In addition, the +procurement of quantitative information allows the use of rigorous statistical methods which would otherwise +be unavailable to the analysis. + +This repository contains in-progress code for the structural analysis of histopathological slides of soay +sheep liver. Analyses occur on two levels of zoom in images. + +Widefield : 2.0 x 3.0 mm +------------------ + +Representative of the general state of the tissue. This is wide enough to see a number of inflammatory foci +and a signficant amount of hepatic tissue. + +- Characteristic scale of sinusoids ( by Gabor filtering ) +- Directionality coefficient ( by Gabor filtering ) +- Information entropy at characteristic scale +- Lacunarity at characteristic scale +- Count of inflammatory foci +- Approximate average size of inflammatory foci +- Ratio of tissue to sinusoids ( a measure of tissue density ) + +Narrowfield : 0.8 x 1.2 mm +-------------------------- + +Images are targetted at hepatic portal triads. + +- Background and inflamed nuclear density +- Count of nuclei ( watershed segmentation for overlapping nuclei ) +- Nuclear radial distribution function +- Dimensions and areas of inflamed portal triads