############ run this part every time before plotting
library('ggplot2')
library('ggpubr')
library('ggsci')
library('data.table')
library('grid')
library('ggthemes')
theme_Publication <- function(base_size=14, base_family="helvetica") {
      (theme_foundation(base_size=base_size, base_family=base_family)
       + theme(plot.title = element_text(size = rel(1.2), hjust = 0.5),
               text = element_text(),
               panel.background = element_rect(colour = NA),
               plot.background = element_rect(colour = NA),
               panel.border = element_rect(colour = NA),
               axis.title = element_text(size = rel(1)),
               axis.title.y = element_text(angle=90,vjust =2),
               axis.title.x = element_text(vjust = -0.2),
               axis.text = element_text(), 
               axis.line = element_line(colour = "black"),
               axis.ticks = element_line(),
               panel.grid.major = element_line(colour="#f0f0f0"),
               panel.grid.minor = element_blank(),
               legend.key = element_rect(colour = NA),
               legend.position = "bottom",#bottom
               legend.direction = "horizontal",#horizontal
               legend.key.size= unit(0.2, "cm"),
               #legend.margin = unit(0, "cm"),
               legend.title = element_text(face="italic"),
               plot.margin=unit(c(10,5,5,5),"mm"),
               strip.background=element_rect(colour="#f0f0f0",fill="#f0f0f0"),
               strip.text = element_text()
          ))
      
}

scale_fill_Publication <- function(...){
      library(scales)
      discrete_scale("fill","Publication",manual_pal(values = c("#386cb0","#fdb462","#7fc97f","#ef3b2c","#662506","#a6cee3","#fb9a99","#984ea3","#ffff33","#006400")), ...)

}

scale_colour_Publication <- function(...){
      library(scales)
      discrete_scale("colour","Publication",manual_pal(values = c("#386cb0","#fdb462","#7fc97f","#ef3b2c","#662506","#a6cee3","#fb9a99","#984ea3","#ffff33","#006400")), ...)

}