--- a +++ b/man/generateXeniumImage.Rd @@ -0,0 +1,43 @@ +% Generated by roxygen2: do not edit by hand +% Please edit documentation in R/import.R +\name{generateXeniumImage} +\alias{generateXeniumImage} +\title{generateXeniumImage} +\usage{ +generateXeniumImage( + dir.path, + increase.contrast = TRUE, + resolution_level = 7, + overwrite_resolution = FALSE, + output.path = NULL, + file.name = "morphology_lowres.tif", + verbose = TRUE, + ... +) +} +\arguments{ +\item{dir.path}{Xenium output folder} + +\item{increase.contrast}{increase the contrast of the image before writing} + +\item{resolution_level}{the level of resolution within Xenium OME-TIFF image. Default: 7 (553x402)} + +\item{overwrite_resolution}{if TRUE, the image "file.name" will be generated again although it exists at "dir.path"} + +\item{output.path}{The path to the new morphology image created if the image should be saved to a location other than Xenium output folder.} + +\item{file.name}{the name of the lowred morphology image. Default: morphology_lowres.tif} + +\item{verbose}{verbose} + +\item{...}{additional parameters passed to the \link{writeImage} function} +} +\description{ +Generate a low resolution DAPI image of the Xenium experiment +} +\details{ +The Xenium morphology_mip.ome.tif file that is found under the outs folder comes is an hyperstack of different resolutions of the DAPI image. +\link{generateXeniumImage} allows extracting only one of these layers by specifying the \code{resolution} parameter (Default: 7 for 553x402) among 1 to 8. +Lower incides of resolutions have higher higher resolutions, e.g. 1 for 35416x25778. Note that you may need to allocate larger memory of Java to import +higher resolution images. +}