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Alyssa Salazar
AlyssaS/
MOVE
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Data: Tabular Time Series Specialty: Endocrinology Laboratory: Blood Tests EHR: Demographics Diagnoses Medications Omics: Genomics Multi-omics Transcriptomics Wearable: Activity Clinical Purpose: Treatment Response Assessment Task: Biomarker Discovery
[c23b31]: / docs / source / tutorial / introduction.rst

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Introduction to MOVE

This guide can help you get started with MOVE.

About the MOVE pipeline

MOVE has the following four steps (also called tasks):

  1. Encoding data: taking input data and converting it into a format MOVE can read.
  2. Tuning the hyperparameters of the VAE model: training multiple models to find the set of hyperparameters that produces the best reconstructions or most stable latent space.
  3. Analyzing the latent space: training a model and inspecting the latent representation it creates.
  4. Identifying associations: using an ensemble of VAE to find associations between the input datasets.

Simulated dataset

For this short tutorial, we provide simulated dataset (available from our GitHub repository). This dataset consists of pretend proteomics and metagenomcis measurements for 500 fictitious individuals. We also report whether these individuals have taken or not 20 imaginary drugs.

All values were randomly generated, but we have added 200 associations between different pairs of drugs and omics features. Let us find these associations with MOVE!

Workspace structure

First, we take a look at how to organize our data and configuration::

tutorial/
│
├── data/
│   ├── changes.small.txt              <- Ground-truth associations (200 links)
│   ├── random.small.drugs.tsv         <- Drug dataset (20 drugs)
│   ├── random.small.ids.tsv           <- Sample IDs (500 samples)
│   ├── random.small.proteomics.tsv    <- Proteomics dataset (200 proteins)
│   └── random.small.metagenomics.tsv  <- Metagenomics dataset (1000 taxa)
│
└── config/                            <- Stores user configuration files
    ├── data/
    │   └── random_small.yaml          <- Configuration to read in the necessary
    │                                     data files.
    ├── experiment/                    <- Configuration for experiments (e.g.,
    │   └── random_small__tune.yaml       for tuning hyperparameters).
    │
    └── task/                          <- Configuration for tasks: such as
        |                                 latent space or identify associations
        │                                 using the t-test or Bayesian approach
        ├── random_small__id_assoc_bayes.yaml
        ├── random_small__id_assoc_ttest.yaml
        └── random_small__latent.yaml

The data directory

All "raw" data files should be placed inside the same directory. These files are TSVs (tab-separated value tables) containing discrete values (e.g., for binary or categorical datasets) or continuous values.

Additionally, make sure each sample has an assigned ID and provide an ID table containing a list of all valid IDs (must appear in every dataset).

The config directory

Configuration is composed and managed by Hydra.

User-defined configuration must be stored in a config folder. This folder can contain a data and task folder to store the configuration files for your dataset and tasks.

Data configuration

Let us take a look at the configuration for our dataset. It is a YAML file, specifying: the directories to look for raw data and store intermediary and final output files, and the list of categorical and continuous datasets we have.

Note that we do not recommend changing the defaults field, otherwise the configuration file will not be properly recognized by MOVE.

Task configuration

Similarly, the task folder contains YAML files to configure the tasks of MOVE. In this tutorial, we provided two examples for running the method to identify associations using our t-test and Bayesian approach, and an example to perform latent space analysis.

For example, for the t-test approach (random_small__id_assoc_ttest.yaml), we define the following values: batch size, number of refits, name of dataset to perturb, target perturb value, configuration for VAE model, and configuration for training loop.

Note that the random_small__id_assoc_bayes.yaml looks pretty similar, but declares a different defaults. This tells MOVE which algorithm to use!

Running MOVE

Encoding data

Make sure you are on the parent directory of the config folder (in this example, it is the tutorial folder), and proceed to run:

>>> cd tutorial
>>> move-dl data=random_small task=encode_data

|:arrow_up:| This command will encode the datasets. The random.small.drugs dataset (defined in config/data/random_small.yaml) will be one-hot encoded, whereas the other two omics datasets will be standardized. Encoded data will be placed in the intermediary folder defined in the :ref:`data config<Data configuration>`.

|:loud_sound:| Every move-dl command will generate a logs folder to store log files timestamping the program's current doings.

Tuning the model's hyperparameters

Once the data has been encoded, we can proceed with the first step of our pipeline: tuning the hyperparameters of our deep learning model. This process can be time-consuming, because several models will be trained and tested. For this short tutorial, you may choose to skip it and proceed to :ref:`analyze the latent space<Analyzing the latent space>`.

Analyzing the latent space

Next, we will train a variational autoencoder and analyze how good it is at reconstructing our input data and generating an informative latent space. Run:

>>> move-dl data=random_small task=random_small__latent

|:arrow_up:| This command will create a latent_space directory in the results folder defined in the :ref:`data config<Data configuration>`. This folder will contain the following plots:

  • Loss curve shows the overall loss, KLD term, binary cross-entropy term, and sum of squared errors term over number of training epochs.
  • Reconstructions metrics boxplot shows a score (accuracy or cosine similarity for categorical and continuous datasets, respectively) per reconstructed dataset.
  • Latent space scatterplot shows a reduced representation of the latent space. To generate this visualization, the latent space is reduced to two dimensions using TSNE (or another user-defined algorithm, e.g., UMAP).
  • Feature importance swarmplot displays the impact perturbing a feature has on the latent space.

Additionally, TSV files corresponding to each plot will be generated. These can be used, for example, to re-create the plots manually or with different styling.

Identifying associations

Next step is to find associations between the drugs taken by each individual and the omics features. Run:

>>> move-dl data=random_small task=random_small__id_assoc_ttest

|:arrow_up:| This command will create a results_sig_assoc.tsv file, listing each pair of associated features and the corresponding median p-value for such association. There should be ~120 associations found. Due to the nature of the method, this number may slightly fluctuate.

|:warning:| Note that the value after task= matches the name of our configuration file. We can create multiple configuration files (for example, changing hyperparameters like learning rate) and call them by their name here.

|:stopwatch:| This command takes approximately 45 min to run on a work laptop (Intel Core i7-10610U @ 1.80 GHz, 32 GB RAM). You can track the progress by checking the corresponding log file created in the logs folder.

If you want to try the Bayesian approach instead, run:

>>> move-dl data=random_small task=random_small__id_assoc_bayes

Again, it should generate similar results with over 120 associations known.

Take a look at the changes.small.txt file and compare your results against it. Did MOVE find any false positives?