--- a +++ b/softwares_config/homer_4.11.config @@ -0,0 +1,77 @@ +HOMER Program Index +Below is a quick introduction to the different programs included in HOMER. Running each program without any arguments will provide basic instructions and a list of command line options. +FASTA file Motif Discovery +findMotifs.pl - performs motif analysis with lists of Gene Identifiers or FASTA files (See FASTA file analysis) + +homer2 - core component of motif finding (Called by everything else , See FASTA file analysis) +Gene/Promoter-based Analysis +findMotifs.pl - performs motif and gene ontology analysis with lists of Gene Identifiers, both promoter and mRNA motifs (See Gene ID Analysis Tutorial) + +findGO.pl - performs only gene ontology analysis with lists of Gene Identifiers (Called by findMotifs.pl, See Gene Ontology Analysis) +loadPromoters.pl - setup custom promoter sets for specialized analysis (See Customization) +Next-Gen Sequencing/Genomic Position Analysis +findMotifsGenome.pl - performs motif analysis from genomic positions (See Finding Motifs from Peaks) + +makeTagDirectory - creates a "tag directory" from high-throughput sequencing alignment files, performs quality control (See Creating a Tag Directory) +makeUCSCfile & makeBigWig.pl - create bedGraph file for visualization with the UCSC Genome Browser (See Creating UCSC file) +findPeaks - find peaks in ChIP-Seq data, regions in histone data, de novo transcripts from GRO-Seq (See Finding ChIP-Seq Peaks) +analyzeChIP-Seq.pl - automation of programs found above (See Automation of ChIP-Seq analysis) + +annotatePeaks.pl - annotation of genomic positions, organization of motif and sequencing data, histograms, heatmaps, and more... (See Annotating Peaks, Quantification) +analyzeRNA.pl - quantification of RNA levels across transcripts (See RNA quantification) +analyzeRepeats.pl - quantification of RNA levels across repeats (See RNA quantification) +getDiffExpression.pl - Calculate differential enrichment of RNA-seq/ChIP-seq/ATAC-seq data (See RNA quantification) +getDifferentialPeaks - find differentially bound regions from ChIP/ATAC-seq experiments +getDifferentialPeaksReplicates.pl - perform peak finding/Differential peak detection taking into account replicates + +mergePeaks - find overlapping peak positions (See Comparing ChIP-Seq Peaks) + +homerTools - basic sequence manipulation (See Sequence Manipulation) +tagDir2bed.pl - output tag directory as an alignment BED file (See Miscellaneous) +bed2pos.pl, pos2bed.pl - convert between HOMER peak file format and BED file format (See Miscellaneous) + +checkPeakFile.pl - use this to see if your peak file is in the correct format +removeOutOfBoundsReads.pl - remove reads found outside acceptable chromosome limits + +annotateTranscripts.pl - annotation of de novo identified transcripts + +Motif Manipulation +compareMotifs.pl - checks a library of motifs for known motifs, creates in HTML output summarizing motif results (described here). +motif2Logo.pl - creates a PNG or PDF logo from any motif file. +revoppMotif.pl - creates a new motif file reflecting the nucleotide preferences of the opposite strand. +seq2profile.pl - creates a new motif file from a consensus sequence + +Hi-C Analysis Programs +analyzeHiC - primary analysis program - generates interaction matrices, normalization, identification of significant interactions, clustering of domains, generates Circos plots (most of the following programs use this one internally, See Hi-C analysis) + +runHiCpca.pl - automated PCA analysis on Hi-C data to identify "compartments" (see Hi-C PCA analysis) +getHiCcorrDiff.pl - calculates the difference in correlation profiles between two Hi-C experiments (see Hi-C PCA analysis) +findHiCCompartments.pl - find continuous or differential regions from PCA/corrDiff results that describe what compartment regions of DNA belong to (see Hi-C PCA analysis) + +findHiCInteractionsByChr.pl - helps automate the finding of high-resolution intra-chromosomal interactions (see Finding Hi-C Interactions) +annotateInteractions.pl - program for re-analysis of significant interactions, such as relating them to ChIP-Seq peaks (see Annotating Interactions) + +SIMA.pl - Novel tool to boost sensitivity by pooling features together when performing interaction calculations. (see SIMA analysis) + +Additional Utilities that may be useful (and sub-programs used by those above) + +addData.pl, addDataHeader.pl, mergeData.pl - tools for joining/merging tab separated flat files + +homerTools extract - extract genomic sequence for peaks from a peak file. +homerTools freq - finds nucleotide/dinucleotide frequencies of a collection of sequence and GC/CpG content. +getPeakTags - finds sequencing tags associated with genomic positions. +scanMotifGenomeWide.pl - look for all instances of a motif in the genome. + + +tagDir2bed.pl - convert *.tags.tsv file directory into a BED file for use with other programs + +homer2 - new motif finding program +homer - original motif finding program (not used anymore) + +getTopPeaks.pl - return peaks with the best peak scores. +getFocalPeaks.pl - return peaks with the highest focus ratios. +assignGenomeAnnotation - assign peaks to specific annotations in the genome + +fasta2tab.pl, tab2fasta.pl - convert between HOMER-style sequence file and a FASTA file. + +changeNewLine.pl - converts mac and dos formated text files (new lines of "\r" and "\r\n") to UNIX style ("\n").