Series GSE283496 Metadata

Series: GSE283496
Query DataSets: for GSE283496
Status: Public on Mar 19, 2025
Title: Genetically Engineered Brain Organoids Recapitulate Spatial and Developmental States of Glioblastoma Progression [scRNA-seq]
Organism: Homo sapiens
Experiment type: Expression profiling by high throughput sequencing
Summary: This study develops engineered glioblastoma organoids (eGBOs) to investigate the role of specific mutations in tumor progression. The analytic framework spans single-cell analysis, spatial transcriptomics, single-cell trajectory analysis, orthotopic implantation, clinically oriented imaging, and histopathological analysis. The work provides an important proof of concept that engineered tumor organoids can model glioblastoma progression.
Overall design: Single-cell transcriptomes from wildtype (WT) and genetically engineered brain organoids harboring glioblastoma-associated mutations (PRO - TERTp C228T / TP53 R248Q; MES - TERTp C228T / NF1 deletion / PTEN deletion). Organoids were assessed 1 month after in vitro differentiation and 3 months after orthotopic xenotransplantation.
Contributor(s): Ishahak M, Kim AH, Millman JR
Citation(s): Ishahak M, Han RH, Annamalai D, Woodiwiss T et al. Genetically Engineered Brain Organoids Recapitulate Spatial and Developmental States of Glioblastoma Progression. Adv Sci (Weinh) 2025 Mar;12(10):e2410110. PMID: 39836549
Submission date: Dec 04, 2024
Last update date: Mar 20, 2025
Contact name: Jeffrey Robert Millman
E-mail(s): jmillman@wustl.edu
Organization name: Washington University in St. Louis
Lab: Jeffrey Millman
Street address: 660 South Euclid
City: St. Louis
State/province: MO
ZIP/Postal code: 63110
Country: USA