| Status |
Public on Mar 19, 2025 |
| Title |
Genetically Engineered Brain Organoids Recapitulate Spatial and Developmental States of Glioblastoma Progression [scRNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study develops engineered glioblastoma organoids (eGBOs) to investigate the role of specific mutations in tumor progression. The analytic framework spans single-cell analysis, spatial transcriptomics, single-cell trajectory analysis, orthotopic implantation, clinically oriented imaging, and histopathological analysis. The work provides an important proof of concept that engineered tumor organoids can model glioblastoma progression.
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| Overall design |
Single-cell transcriptomes from wildtype (WT) and genetically engineered brain organoids harboring glioblastoma-associated mutations (PRO - TERTp C228T / TP53 R248Q; MES - TERTp C228T / NF1 deletion / PTEN deletion). Organoids were assessed 1 month after in vitro differentiation and 3 months after orthotopic xenotransplantation.
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| Contributor(s) |
Ishahak M, Kim AH, Millman JR |
| Citation(s) |
- Ishahak M, Han RH, Annamalai D, Woodiwiss T et al. Genetically Engineered Brain Organoids Recapitulate Spatial and Developmental States of Glioblastoma Progression. Adv Sci (Weinh) 2025 Mar;12(10):e2410110. PMID: 39836549
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| Submission date |
Dec 04, 2024 |
| Last update date |
Mar 20, 2025 |
| Contact name |
Jeffrey Robert Millman |
| E-mail(s) |
jmillman@wustl.edu
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| Organization name |
Washington University in St. Louis
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| Lab |
Jeffrey Millman
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| Street address |
660 South Euclid
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| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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