<table><tbody><tr><td><table cellpadding="2" cellspacing="0" width="600"><tbody><tr bgcolor="#cccccc" valign="top"><td colspan="2"><table width="600"><tbody><tr><td><strong class="acc" id="GSE174858"><a href="/geo/query/acc.cgi?acc=GSE174858" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)">Series GSE174858</a></strong></td>

<td></td>

<td align="right" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_gds)"><a href="/gds/?term=GSE174858[Accession]">Query DataSets for GSE174858</a></td>

</tr></tbody></table></td></tr>

<tr valign="top"><td>Status</td>

<td>Public on May 22, 2021</td>

</tr>

<tr valign="top"><td nowrap="">Title</td>

<td style="text-align: justify">An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies</td>

</tr>

<tr valign="top"><td nowrap="">Organism</td>

<td><a href="/Taxonomy/Browser/wwwtax.cgi?mode=Info&amp;id=9823" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_organismus)">Sus scrofa</a></td>

</tr>

<tr valign="top"><td nowrap="">Experiment type</td>

<td>Expression profiling by high throughput sequencing<br></td>

</tr>

<tr valign="top"><td nowrap="">Summary</td>

<td style="text-align: justify">Conventional in vitro methods for biological evaluation of intra-arterial devices such as stents fail to accurately predict cytotoxicity and remodeling events. An ex vivo flow-tunable vascular bioreactor system (VesselBRx), comprising intra- and extra-luminal monitoring capabilities, addresses these limitations. VesselBRx mimics the in vivo physiological, hyperplastic, and cytocompatibility events of absorbable magnesium (Mg)-based stents in ex vivo stent-treated porcine and human coronary arteries, with in-situ and real-time monitoring of local stent degradation effects. Unlike conventional, static cell culture, the VesselBRx perfusion system eliminates unphysiologically high intracellular Mg2+ concentrations and localized O2 consumption resulting from stent degradation. Whereas static stented arteries exhibited only 20.1% cell viability and upregulated apoptosis, necrosis, metallic ion, and hypoxia-related gene signatures, stented arteries in VesselBRx showed almost identical cell viability to in vivo rabbit models (~94.0%). Hyperplastic intimal remodeling developed in unstented arteries subjected to low shear stress, but was inhibited by Mg-based stents in VesselBRx, similarly to in vivo. VesselBRx represents a critical advance from the current static culture standard of testing absorbable vascular implants.<br></td>

</tr>

<tr valign="top"><td nowrap="">&nbsp;</td>

<td></td>

</tr>

<tr valign="top"><td nowrap="">Overall design</td>

<td style="text-align: justify">Vascular mRNA profiles of native, bioreactor static stented (Stent/Flow-), bioreactor dynamic stented (Stent/Flow++), bioreactor dynamic no-stent (Flow++), and bioreactor dynamic low flow no-stent (Flow+) porcine coronary arteries<br></td>

</tr>

<tr valign="top"><td nowrap="">&nbsp;</td>

<td></td>

</tr>

<tr valign="top"><td>Contributor(s)</td>

<td><a href="/pubmed/?term=Wang J[Author]">Wang J</a>, <a href="/pubmed/?term=Wu J[Author]">Wu J</a></td>

</tr>

<tr valign="top"><td nowrap="">Citation(s)</td>

<td><span class="pubmed_id" id="34087584"><!--?xml version="1.0" encoding="UTF-8"?-->

<div class="PubmedCitation"><ul><li><span class="authors">Wang J, Kural MH, Wu J, Leiby KL et al. </span><span class="title">An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies. </span><span class="source">Biomaterials</span> 2021 Aug;275:120911. PMID:&nbsp;<a title="Link to PubMed record" href="https://www.ncbi.nlm.nih.gov/pubmed/34087584">34087584</a></li></ul></div>

</span></td>

</tr>

<tr valign="top"><td nowrap=""></td>

<td style="text-align: justify"><a href="https://doi.org/10.1016/j.biomaterials.2021.120911Get">https://doi.org/10.1016/j.biomaterials.2021.120911Get</a><br></td>

</tr>

<tr valign="top"><td colspan="2"><span id="geo2r"></span> <span id="rnaseq_counts"></span></td></tr>

<tr bgcolor="#eeeeee" valign="top"><td>Submission date</td>

<td>May 21, 2021</td>

</tr>

<tr bgcolor="#eeeeee" valign="top"><td>Last update date</td>

<td>Jun 24, 2022</td>

</tr>

<tr bgcolor="#eeeeee" valign="top"><td>Contact name</td>

<td>Juan Wang</td>

</tr>

<tr bgcolor="#eeeeee" valign="top"><td nowrap="">E-mail(s)</td>

<td><a href="mailto:Juan.wang@yale.edu">Juan.wang@yale.edu</a><br></td>

</tr>

<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Organization name</td>

<td style="text-align: justify">Yale University<br></td>

</tr>

<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Street address</td>

<td style="text-align: justify">10 Amistad Street<br></td>

</tr>

<tr bgcolor="#eeeeee" valign="top"><td nowrap="">City</td>

<td style="text-align: justify">New Haven</td>

</tr>

<tr bgcolor="#eeeeee" valign="top"><td nowrap="">State/province</td>

<td style="text-align: justify">CT</td>

</tr>

<tr bgcolor="#eeeeee" valign="top"><td nowrap="">ZIP/Postal code</td>

<td style="text-align: justify">06510</td>

</tr>

<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Country</td>

<td style="text-align: justify">USA</td>

</tr>

<tr valign="top"><td nowrap="">&nbsp;</td>

<td></td>

</tr>

</tbody></table>