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| Status |
Public on May 22, 2021 |
| Title |
An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Conventional in vitro methods for biological evaluation of intra-arterial devices such as stents fail to accurately predict cytotoxicity and remodeling events. An ex vivo flow-tunable vascular bioreactor system (VesselBRx), comprising intra- and extra-luminal monitoring capabilities, addresses these limitations. VesselBRx mimics the in vivo physiological, hyperplastic, and cytocompatibility events of absorbable magnesium (Mg)-based stents in ex vivo stent-treated porcine and human coronary arteries, with in-situ and real-time monitoring of local stent degradation effects. Unlike conventional, static cell culture, the VesselBRx perfusion system eliminates unphysiologically high intracellular Mg2+ concentrations and localized O2 consumption resulting from stent degradation. Whereas static stented arteries exhibited only 20.1% cell viability and upregulated apoptosis, necrosis, metallic ion, and hypoxia-related gene signatures, stented arteries in VesselBRx showed almost identical cell viability to in vivo rabbit models (~94.0%). Hyperplastic intimal remodeling developed in unstented arteries subjected to low shear stress, but was inhibited by Mg-based stents in VesselBRx, similarly to in vivo. VesselBRx represents a critical advance from the current static culture standard of testing absorbable vascular implants.
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| Overall design |
Vascular mRNA profiles of native, bioreactor static stented (Stent/Flow-), bioreactor dynamic stented (Stent/Flow++), bioreactor dynamic no-stent (Flow++), and bioreactor dynamic low flow no-stent (Flow+) porcine coronary arteries
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| Contributor(s) |
Wang J, Wu J |
| Citation(s) |
- Wang J, Kural MH, Wu J, Leiby KL et al. An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies. Biomaterials 2021 Aug;275:120911. PMID: 34087584
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https://doi.org/10.1016/j.biomaterials.2021.120911Get
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| Submission date |
May 21, 2021 |
| Last update date |
Jun 24, 2022 |
| Contact name |
Juan Wang |
| E-mail(s) |
Juan.wang@yale.edu
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| Organization name |
Yale University
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| Street address |
10 Amistad Street
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| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06510 |
| Country |
USA |
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