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| Status |
Public on Jan 21, 2025 |
| Title |
Comparison of transcriptional profiles of human psoriatic skin and skin from K14-CreER/GPX4flox mouse model. |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Transcriptional profile of full thickness skin or epidermis only obtained from K14-CreER+/+;GPX4flox mouse model vs K14-CreER-/-;GPX4flox controls is analyzed and compared to transcriptional profile of epidermis plus papillary dermis obtained from patient-matched lesional vs perilesional psoriatic skin.
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| Overall design |
GPX4 was selectively deleted in a subset of K14+ keratinocytes of 8-10 week-old B6.K14-CreER+/+;GPX4flox male and female mice using tamoxifen injections. On day 15-18 after the initiation of tamoxifen injections, either full thickness skin (epidermis and dermis, 3 mice per group) or epidermis only (3 mice per group) were harvested from K14-CreER+/+;GPX4flox mice and K14-CreER-/-;GPX4flox control mice, and used for RNA-sequencing. In addition, clinical specimens composed of epidermis and superficial dermis were obtained from three patients suffering from psoriasis. Specifically, 3 lesional (from 2 males, 1 female) and 3 patient-matched perilesional (located 8 mm from the active lesion) specimens were obtained from the back, thigh or leg, and used for RNA-sequencing.
NOTE FROM SUBMITTER: Due to privacy rules and logistics we will only be uploading the processed raw counts file for the human data.
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| Contributor(s) |
Bunimovich Y, Vats K |
| Citation(s) |
- Vats K, Tian H, Singh K, Tyurina YY et al. Ferroptosis of select skin epithelial cells initiates and maintains chronic systemic immune-mediated psoriatic disease. J Clin Invest 2024 Nov 21;135(2). PMID: 39570671
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| Submission date |
Jun 27, 2023 |
| Last update date |
Jan 22, 2025 |
| Contact name |
Uma Chandran |
| E-mail(s) |
chandran@pitt.edu
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| Organization name |
University of Pittsburgh
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| Department |
Biomedical Informatics
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| Street address |
5607 Baum Blvd
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| City |
PITTSBURGH |
| State/province |
PA |
| ZIP/Postal code |
15206 |
| Country |
USA |
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