This DATSETNAMEreadme.txt file was generated on 2022-05-18 by Li Liu

GENERAL INFORMATION

1. Title of Dataset: Datasets used in the manuscript "Early detection of cerebrovascular pathology and protective antiviral immunity by MRI"

2. Author Information
	A. Principal Investigator Contact Information
		Name: Li Liu
		Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health
		Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA
		Email: li.liu3@nih.gov

		Name: Alan P. Koretsky
		Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health
		Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA
		Email: koretskya@ninds.nih.gov

	B. Associate or Co-investigator Contact Information
		Name: Stephen Dodd
		Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health
		Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 
		Email: doddst@ninds.nih.gov

		Name: Ryan D. Hunt
		Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health
		Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 
		Email: Ryan.Hunt@uvm.edu

		Name: Nikorn Pothayee
		Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health
		Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 
		Email: nikorn.pothayee@nih.gov

		Name: Tatjana Atanasijevic
		Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health
		Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 
		Email: tatjana.atanasijevic@nih.gov

		Name: Nadia Bouraoud
		Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health
		Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 
		Email: Nadia.Bouraoud@nih.gov

		Name: Dragan Maric
		Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health
		Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 
		Email: maricd@ninds.nih.gov

		Name: E. Ashley Moseman
		Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health
		Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 
		Email: ashley.moseman@duke.edu

		Name: Selamawit Gossa
		Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health
		Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 
		Email: selamawit.gossa@nih.gov

		Name: Dorian B. McGavern
		Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health
		Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 
		Email: mcgavernd@ninds.nih.gov

	C. Alternate Contact Information
		Name: E. Ashley Moseman
		Institution: Duke University School of Medicine
		Address: Durham, NC 27710, USA 
		Email: ashley.moseman@duke.edu

3. Date of data collection: From 2017-12-01 to 2022-03-02

4. Geographic location of data collection: Bethesda, MD, USA

5. Information about funding sources that supported the collection of the data: 
	The Intramural Program of the National Institute of Neurological Disorders and Stroke (1ZIANS003047-15) for Alan P Koretsky
	The Intramural Program of the National Institute of Neurological Disorders and Stroke (1ZIANS003112-13) for Dorian B McGavern

DATA & FILE OVERVIEW
File List: 
	Method of Data Collection.docx - Description of experimental methodology that used in this study.
	Source Data Fig 1.xlsx - Quantification of volume of hypointensity in the turbinates and numbers of hypointensity spots in the OB on day 4, 6, and 11. 
	Source Data Fig 2.xlsx - Quantification of numbers of hypointensity spots in the brain (not including OB) on day 4, 6, and 11.
	Source Data Fig 3.xlsx - Quantification of IHC staining showed increased expression of endothelial VCAM-1 and ICAM-1 in the OB on day 6 of infection. Quantification of the volume of bleeding in the turbinates and the numbers of hypointensity spots in the OB and brain on day 6, in the isotype control group and anti-LFA-1/VLA-4 antibodies treated group.
	Source Data Fig 5.xlsx - Quantification of volume of bleeding in the turbinates and numbers of hypointensity spots in the OB and brain without or with T cells early and late transfer 
	Source Data Fig 6.xlsx - Quantification of IHC study verified that antiviral CD8 T cells reduced VSV-induced brain bleeding.
	Source Data Fig 8.xlsx - Quantification of IHC study showing the activation of vessel endothelium cells and microglia were found at the ONL/GL and MCL/GCL on day 1. 
	Source Data Figure 3-figure supplement 1.xlsx - Quantification of IHC staining showed increased expression of endothelial VCAM-1 and ICAM-1 in the OB and thalamus on day 6 of infection.
	Source Data Figure 6-figure supplement 1.xlsx - Quantification of bleeding volume in turbinates, and number of hypointensity spots in OB and brain without or with T cell transfer (5 x 105 T cells, and 3 x 106 T cells) on day 11.
	Source Data Figure 8-figure supplement 2.xlsx - Quantification of the number of hypointensity spots in OB and brain on day 6 and 11, upon transfer of unlabeled CD8 T cells or MPIO-labeled CD8 T cells.

METHODOLOGICAL INFORMATION
	Method of Data Collection.docx