This DATSETNAMEreadme.txt file was generated on 2022-05-18 by Li Liu

GENERAL INFORMATION

1. Title of Dataset: Datasets used in the manuscript "Early detection of cerebrovascular pathology and protective antiviral immunity by MRI"

2. Author Information

    A. Principal Investigator Contact Information

        Name: Li Liu

        Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health

        Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA

        Email: li.liu3@nih.gov

        Name: Alan P. Koretsky

        Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health

        Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA

        Email: koretskya@ninds.nih.gov

    B. Associate or Co-investigator Contact Information

        Name: Stephen Dodd

        Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health

        Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 

        Email: doddst@ninds.nih.gov

        Name: Ryan D. Hunt

        Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health

        Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 

        Email: Ryan.Hunt@uvm.edu

        Name: Nikorn Pothayee

        Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health

        Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 

        Email: nikorn.pothayee@nih.gov

        Name: Tatjana Atanasijevic

        Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health

        Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 

        Email: tatjana.atanasijevic@nih.gov

        Name: Nadia Bouraoud

        Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health

        Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 

        Email: Nadia.Bouraoud@nih.gov

        Name: Dragan Maric

        Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health

        Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 

        Email: maricd@ninds.nih.gov

        Name: E. Ashley Moseman

        Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health

        Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 

        Email: ashley.moseman@duke.edu

        Name: Selamawit Gossa

        Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health

        Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 

        Email: selamawit.gossa@nih.gov

        Name: Dorian B. McGavern

        Institution: National Institute of Neurological Disorders and Stroke, National Institutes of Health

        Address: 10 Center Drive, Building 10, Bethesda, MD 20892, USA 

        Email: mcgavernd@ninds.nih.gov

    C. Alternate Contact Information

        Name: E. Ashley Moseman

        Institution: Duke University School of Medicine

        Address: Durham, NC 27710, USA 

        Email: ashley.moseman@duke.edu

3. Date of data collection: From 2017-12-01 to 2022-03-02

4. Geographic location of data collection: Bethesda, MD, USA

5. Information about funding sources that supported the collection of the data: 

    The Intramural Program of the National Institute of Neurological Disorders and Stroke (1ZIANS003047-15) for Alan P Koretsky

    The Intramural Program of the National Institute of Neurological Disorders and Stroke (1ZIANS003112-13) for Dorian B McGavern

DATA & FILE OVERVIEW

File List: 

    Method of Data Collection.docx - Description of experimental methodology that used in this study.

    Source Data Fig 1.xlsx - Quantification of volume of hypointensity in the turbinates and numbers of hypointensity spots in the OB on day 4, 6, and 11. 

    Source Data Fig 2.xlsx - Quantification of numbers of hypointensity spots in the brain (not including OB) on day 4, 6, and 11.

    Source Data Fig 3.xlsx - Quantification of IHC staining showed increased expression of endothelial VCAM-1 and ICAM-1 in the OB on day 6 of infection. Quantification of the volume of bleeding in the turbinates and the numbers of hypointensity spots in the OB and brain on day 6, in the isotype control group and anti-LFA-1/VLA-4 antibodies treated group.

    Source Data Fig 5.xlsx - Quantification of volume of bleeding in the turbinates and numbers of hypointensity spots in the OB and brain without or with T cells early and late transfer 

    Source Data Fig 6.xlsx - Quantification of IHC study verified that antiviral CD8 T cells reduced VSV-induced brain bleeding.

    Source Data Fig 8.xlsx - Quantification of IHC study showing the activation of vessel endothelium cells and microglia were found at the ONL/GL and MCL/GCL on day 1. 

    Source Data Figure 3-figure supplement 1.xlsx - Quantification of IHC staining showed increased expression of endothelial VCAM-1 and ICAM-1 in the OB and thalamus on day 6 of infection.

    Source Data Figure 6-figure supplement 1.xlsx - Quantification of bleeding volume in turbinates, and number of hypointensity spots in OB and brain without or with T cell transfer (5 x 105 T cells, and 3 x 106 T cells) on day 11.

    Source Data Figure 8-figure supplement 2.xlsx - Quantification of the number of hypointensity spots in OB and brain on day 6 and 11, upon transfer of unlabeled CD8 T cells or MPIO-labeled CD8 T cells.

METHODOLOGICAL INFORMATION

    Method of Data Collection.docx