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| Status |
Public on Oct 24, 2024 |
| Title |
Single-cell transcriptomics of pediatric Burkitt lymphoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Burkitt lymphoma (BL) is the most frequent B-cell lymphoma in pediatric patients. While most patients are currently cured, a fraction of them are resistant to therapy. In order to investigate BL heterogeneity and the features distinguishing the non-responders (NR) to therapy, we analyzed pediatric EBV-negative BL specimens collected at diagnosis by single-cell (sc)-transcriptomics. Analysis of the non-tumor component revealed a predominance of immune cell infiltrates and a small representation of fibroblasts that was enriched in NR. Tumor cells displayed patient-specific features, as well as subpopulations that were common to most patients and expressed transcripts related to cell cycle, signaling pathways and cell-of-origin signatures.
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| Overall design |
Paired single-cell transcriptomics and immunoglobulin repertoire analyses were performed on 12 sporadic EBV-negative BL specimens (11 patients), including 8 pleural or abdominal effusions and 4 nodal tumor masses, which were collected at diagnosis and frozen as viable single cell suspensions.
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| Contributor(s) |
Basso K, Holmes AB, Mussolin L, Dalla-Favera R |
| Citation(s) |
- Corinaldesi C, Holmes AB, Martire G, Tosato A et al. Single-cell transcriptomics of pediatric Burkitt lymphoma reveals intra-tumor heterogeneity and markers of therapy resistance. Leukemia 2025 Jan;39(1):189-198. PMID: 39424708
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| Submission date |
Aug 07, 2023 |
| Last update date |
Oct 24, 2024 |
| Contact name |
Riccardo Dalla-Favera |
| E-mail(s) |
rd10@cumc.columbia.edu
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| Organization name |
Columbia University
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| Street address |
1130 St Nicholas Avenue
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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