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+<table><tbody><tr><td><table cellpadding="2" cellspacing="0" width="600"><tbody><tr bgcolor="#cccccc" valign="top"><td colspan="2"><table width="600"><tbody><tr><td><strong class="acc" id="GSE275043"><a href="/geo/query/acc.cgi?acc=GSE275043" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)">Series GSE275043</a></strong></td>
+<td></td>
+<td align="right" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_gds)"><a href="/gds/?term=GSE275043[Accession]">Query DataSets for GSE275043</a></td>
+</tr></tbody></table></td></tr>
+<tr valign="top"><td>Status</td>
+<td>Public on Mar 19, 2025</td>
+</tr>
+<tr valign="top"><td nowrap="">Title</td>
+<td style="text-align: justify">Genome-wide CRISPR screen identifies IRF1 and TFAP4 as transcriptional regulators of Galectin-9 in T cell acute lymphoblastic leukemia [CRISPR]</td>
+</tr>
+<tr valign="top"><td nowrap="">Organism</td>
+<td><a href="/Taxonomy/Browser/wwwtax.cgi?mode=Info&amp;id=9606" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_organismus)">Homo sapiens</a></td>
+</tr>
+<tr valign="top"><td nowrap="">Experiment type</td>
+<td>Other<br></td>
+</tr>
+<tr valign="top"><td nowrap="">Summary</td>
+<td style="text-align: justify">Galectin-9 is overexpressed in a variety of cancers and associated with worse clinical outcome in some cancers. However, the regulators driving Galectin-9 expression are unknown. Here, we defined the transcriptional regulators and epigenetic circuitry of Galectin-9 in pediatric T cell acute lymphoblastic leukemia (T-ALL), as an example of a disease with strong Galectin-9 expression, in which higher expression was associated with lower overall survival. By performing a genome-wide CRISPR screen, we identified the transcription factors IRF1 and TFAP4 as key regulators for Galectin-9 expression by binding its regulatory elements. While IRF1 was observed exclusively on the promoter, TFAP4 binding was detected at an enhancer solely in T-ALL cells associated with higher Galectin-9 levels. Together, our results show that IRF1 is responsible and indispensable for Galectin-9 expression and TFAP4 further fine-tunes its expression. Our approach, a flow-based genome-wide CRISPR screen complemented by transcription factor binding and enhancer mapping, creates innovative opportunities for understanding and manipulating epigenetic transcriptional regulation in cancer.<br></td>
+</tr>
+<tr valign="top"><td nowrap="">&nbsp;</td>
+<td></td>
+</tr>
+<tr valign="top"><td nowrap="">Overall design</td>
+<td style="text-align: justify">Genome-wide CRISPR screen (Brunello library) of Galectin-9 in T-ALL cell line Jurkat<br></td>
+</tr>
+<tr valign="top"><td nowrap="">&nbsp;</td>
+<td></td>
+</tr>
+<tr valign="top"><td>Contributor(s)</td>
+<td><a href="/pubmed/?term=Wiggers CR[Author]">Wiggers CR</a>, <a href="/pubmed/?term=Yüzügüldü B[Author]">Yüzügüldü B</a>, <a href="/pubmed/?term=Lohr JG[Author]">Lohr JG</a>, <a href="/pubmed/?term=Knoechel B[Author]">Knoechel B</a></td>
+</tr>
+<tr valign="top"><td>Citation missing</td>
+<td class="message" id="pmidCell">Has this study been published? Please <a href="/geo/submitter">login</a> to update or <a href="mailto:geo@ncbi.nlm.nih.gov?subject=Citation for GSE275043 [not logged in]&amp;body=Thank you very much for helping to keep GEO citation links up-to-date. Please enter the PubMed ID (PMID) or full citation for GSE275043 below, and we will update the record accordingly within the next day or two. The PubMed ID or citation is:
+">notify GEO</a>.</td>
+</tr>
+<tr valign="top"><td colspan="2"><span id="geo2r"></span> <span id="rnaseq_counts"></span></td></tr>
+<tr bgcolor="#eeeeee" valign="top"><td>Submission date</td>
+<td>Aug 16, 2024</td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td>Last update date</td>
+<td>Mar 20, 2025</td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td>Contact name</td>
+<td>Birgit Knoechel</td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td nowrap="">E-mail(s)</td>
+<td><a href="mailto:birgit.knoechel@hci.utah.edu">birgit.knoechel@hci.utah.edu</a><br></td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Organization name</td>
+<td style="text-align: justify">HCI<br></td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Street address</td>
+<td style="text-align: justify">2000 Circle of Hope<br></td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td nowrap="">City</td>
+<td style="text-align: justify">Salt Lake City</td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td nowrap="">ZIP/Postal code</td>
+<td style="text-align: justify">84112</td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Country</td>
+<td style="text-align: justify">USA</td>
+</tr>
+<tr valign="top"><td nowrap="">&nbsp;</td>
+<td></td>
+</tr>
+<tr valign="top"><td>Platforms (1)</td>
+<td onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)"><table cellpadding="3" style="position:relative;top:-5px;left:-5px"><tbody><tr><td valign="top"><a href="/geo/query/acc.cgi?acc=GPL18573" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)">GPL18573</a></td>
+<td valign="top">Illumina NextSeq 500 (Homo sapiens)</td>
+</tr></tbody></table></td>
+</tr>
+</td></tr></tbody></table>
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