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| Status |
Public on Jan 09, 2025 |
| Title |
PDX models of relapsed pediatric AML preserve global gene expression patterns and reveal therapeutic targets |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Patient-derived xenograft (PDX) models are commonly used for preclinical evaluation of targeted therapies. It is important to consider the fidelity with which these systems recapitulate the patient disease state. Currently, there is little information regarding how well pediatric AML PDXs mimic the global gene expression found in patients. Here we analyzed RNAseq data from a diverse series of high-risk pediatric AML PDXs, separately and compared to primary patient data. Unsupervised clustering of PDX data resulted in segregation according to KMT2A (MLL) status. Combined analysis showed PDX samples aligned with patient samples harboring similar genetics. Among KMT2A rearranged samples, we observed strong correlation of expression levels of nearly all expressed transcripts. Furthermore, matched patient/PDX pairs showed strong concordance, suggesting retention of sample-specific gene expression. Interestingly, our analysis uncovered previously unidentified cryptic CBFA2T3-GLIS2 rearrangement in two PDX models. Based on high BCL2 mRNA in these models, we tested the efficacy of venetoclax in combination with chemotherapy. While standard daunorubicin/ara-C treatment failed to produce benefits, CPX-351 decreased disease burden and prolonged survival, particularly when combined with venetoclax. These results validate PDX modeling of high-risk pediatric AML and highlight this system's utility for pre-clinical therapeutic discovery, especially for rare subtypes of disease.
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| Overall design |
We generated a series of PDX models from pediatric AML patient specimens and obtained RNA from BM of mice with high levels of disease. We performed single end RNA sequencing and compared gene expression to samples selected from the NCI TARGET database as well as to residual matched patient samples. PDX models generated in parallel in NSG and NSGS mice were also compared to each other. We identified gene expression profiles associated with MLL and GLIS2 rearrangements in order to identify potential therapeutic strategies.
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| Contributor(s) |
Wunderlich M, Chen J, Mizukawa B |
| Citation missing |
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| Submission date |
Jan 10, 2024 |
| Last update date |
Jan 09, 2025 |
| Contact name |
Mark Wunderlich |
| Organization name |
Cincinnati Children's Hospital Medical Center
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| Street address |
3333 Burnet Ave
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| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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