--- a
+++ b/README.md
@@ -0,0 +1,82 @@
+<table><tbody><tr><td><table cellpadding="2" cellspacing="0" width="600"><tbody><tr bgcolor="#cccccc" valign="top"><td colspan="2"><table width="600"><tbody><tr><td><strong class="acc" id="GSE255802"><a href="/geo/query/acc.cgi?acc=GSE255802" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)">Series GSE255802</a></strong></td>
+<td></td>
+<td align="right" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_gds)"><a href="/gds/?term=GSE255802[Accession]">Query DataSets for GSE255802</a></td>
+</tr></tbody></table></td></tr>
+<tr valign="top"><td>Status</td>
+<td>Public on Feb 17, 2025</td>
+</tr>
+<tr valign="top"><td nowrap="">Title</td>
+<td style="text-align: justify">Transcriptional and epigenetic rewiring by the NUP98::KDM5A fusion oncoprotein directly activates CDK12 (Cut&amp;Tag)</td>
+</tr>
+<tr valign="top"><td nowrap="">Organisms</td>
+<td><a href="/Taxonomy/Browser/wwwtax.cgi?mode=Info&amp;id=9606" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_organismus)">Homo sapiens</a>; <a href="/Taxonomy/Browser/wwwtax.cgi?mode=Info&amp;id=10090" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_organismus)">Mus musculus</a></td>
+</tr>
+<tr valign="top"><td nowrap="">Experiment type</td>
+<td>Genome binding/occupancy profiling by high throughput sequencing<br></td>
+</tr>
+<tr valign="top"><td nowrap="">Summary</td>
+<td style="text-align: justify">Nucleoporin 98 (NUP98) fusion oncoproteins are strong drivers of pediatric acute myeloid leukemia (AML) with poor prognosis. Here we show that NUP98 fusion-expressing AML harbors an epigenetic signature that is characterized by increased accessibility of hematopoietic stem cell genes and enrichment of activating histone marks. We employ an AML model for ligand-induced degradation of the NUP98::KDM5A fusion oncoprotein to identify epigenetic programs and transcriptional targets that are directly regulated by NUP98::KDM5A through CUT&amp;Tag and nascent RNA-seq. Orthogonal genome-wide CRISPR/Cas9 screening identifies 12 direct NUP98::KDM5A target genes, which are essential for AML cell growth. Among these, we validate cyclin-dependent kinase 12 (CDK12) as a druggable vulnerability in NUP98::KDM5A-expressing AML. In line with its role in the transcription of DNA damage repair genes, small-molecule-mediated CDK12 inactivation causes increased DNA damage, leading to AML cell death. Altogether, we show that NUP98::KDM5A directly regulates a core set of essential target genes and reveal CDK12 as an actionable vulnerability in AML with oncogenic NUP98 fusions.<br></td>
+</tr>
+<tr valign="top"><td nowrap="">&nbsp;</td>
+<td></td>
+</tr>
+<tr valign="top"><td nowrap="">Overall design</td>
+<td style="text-align: justify">Cut&amp;Tag: H3K27ac and H3K4me3 CUT&amp;Tag was perfromed in primary material from a NUP98::KDM5A patient-derived xenograft model and in a dTAG-NUP98::KDM5A AML cell line that was treated with dTAG13 for 8h to investigate changes in histone mark distribution upon fusion protein degradation.<br></td>
+</tr>
+<tr valign="top"><td nowrap="">&nbsp;</td>
+<td></td>
+</tr>
+<tr valign="top"><td>Contributor(s)</td>
+<td><a href="/pubmed/?term=Troester S[Author]">Troester S</a>, <a href="/pubmed/?term=Eder T[Author]">Eder T</a>, <a href="/pubmed/?term=Wukowits N[Author]">Wukowits N</a>, <a href="/pubmed/?term=Grebien F[Author]">Grebien F</a></td>
+</tr>
+<tr valign="top"><td>Citation missing</td>
+<td class="message" id="pmidCell">Has this study been published? Please <a href="/geo/submitter">login</a> to update or <a href="mailto:geo@ncbi.nlm.nih.gov?subject=Citation for GSE255802 [not logged in]&amp;body=Thank you very much for helping to keep GEO citation links up-to-date. Please enter the PubMed ID (PMID) or full citation for GSE255802 below, and we will update the record accordingly within the next day or two. The PubMed ID or citation is:
+">notify GEO</a>.</td>
+</tr>
+<tr valign="top"><td colspan="2"><span id="geo2r"></span> <span id="rnaseq_counts"></span></td></tr>
+<tr bgcolor="#eeeeee" valign="top"><td>Submission date</td>
+<td>Feb 14, 2024</td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td>Last update date</td>
+<td>Feb 18, 2025</td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td>Contact name</td>
+<td>Thomas Eder</td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td nowrap="">E-mail(s)</td>
+<td><a href="mailto:Thomas.Eder@vetmeduni.ac.at">Thomas.Eder@vetmeduni.ac.at</a><br></td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Organization name</td>
+<td style="text-align: justify">University of Veterinary Medicine, Vienna<br></td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Department</td>
+<td style="text-align: justify">Department for Biological Sciences and Pathobiology<br></td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Lab</td>
+<td style="text-align: justify">Grebien Lab<br></td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Street address</td>
+<td style="text-align: justify">Veterinaerplatz 1<br></td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td nowrap="">City</td>
+<td style="text-align: justify">Vienna</td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td nowrap="">ZIP/Postal code</td>
+<td style="text-align: justify">A-1210</td>
+</tr>
+<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Country</td>
+<td style="text-align: justify">Austria</td>
+</tr>
+<tr valign="top"><td nowrap="">&nbsp;</td>
+<td></td>
+</tr>
+<tr valign="top"><td>Platforms (2)</td>
+<td onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)"><table cellpadding="3" style="position:relative;top:-5px;left:-5px"><tbody><tr><td valign="top"><a href="/geo/query/acc.cgi?acc=GPL30172" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)">GPL30172</a></td>
+<td valign="top">NextSeq 2000 (Mus musculus)</td>
+</tr>
+<tr><td valign="top"><a href="/geo/query/acc.cgi?acc=GPL30173" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)">GPL30173</a></td>
+<td valign="top">NextSeq 2000 (Homo sapiens)</td>
+</tr>
+</tbody></table></td>
+</tr>
+</td></tr></tbody></table>
\ No newline at end of file