<table><tbody><tr><td><table cellpadding="2" cellspacing="0" width="600"><tbody><tr bgcolor="#cccccc" valign="top"><td colspan="2"><table width="600"><tbody><tr><td><strong class="acc" id="GSE293716"><a href="/geo/query/acc.cgi?acc=GSE293716" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)">Series GSE293716</a></strong></td>

<td></td>

<td align="right" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_gds)"><a href="/gds/?term=GSE293716[Accession]">Query DataSets for GSE293716</a></td>

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<tr valign="top"><td>Status</td>

<td>Public on Apr 03, 2025</td>

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<tr valign="top"><td nowrap="">Title</td>

<td style="text-align: justify">Stress attenuation by the adrenergic-specific lncRNA NESPR prevents cell death in neuroblastoma cells [ATAC-seq]</td>

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<tr valign="top"><td nowrap="">Organism</td>

<td><a href="/Taxonomy/Browser/wwwtax.cgi?mode=Info&amp;id=9606" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_organismus)">Homo sapiens</a></td>

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<tr valign="top"><td nowrap="">Experiment type</td>

<td>Genome binding/occupancy profiling by high throughput sequencing<br></td>

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<tr valign="top"><td nowrap="">Summary</td>

<td style="text-align: justify">Neuroblastoma is a pediatric cancer of the sympathetic nervous system characterized by heterogeneous cell states that mirror normal differentiation trajectories. Each state is governed by a core regulatory transcriptional circuitry that reinforces cell identity through an autoregulatory feedforward loop. We identified the long non-coding RNA NESPR as specifically expressed in adrenergic neuroblastoma cells. NESPR expression correlates with high-risk neuroblastoma clinical parameters and poor patient survival. NESPR is located within an insulated gene neighborhood alongside PHOX2B, a master transcription factor of the adrenergic identity. NESPR depletion reduced cell proliferation and increased caspase activity in neuroblastoma cell lines, and NESPR knockout in a neuroblastoma zebrafish model led to reduced tumor penetrance. Subcellular localization revealed NESPR to be a cytosolic long non-coding RNA, suggesting a trans-regulatory function. RNA-sequencing following NESPR depletion revealed a shift from an adrenergic to a mesenchymal cell state, due to proteotoxic stress-induced molecular reprogramming. These findings suggest NESPR as a regulator of neuroblastoma cell identity with potential therapeutic opportunities in high-risk neuroblastoma cases.<br></td>

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<tr valign="top"><td nowrap="">&nbsp;</td>

<td></td>

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<tr valign="top"><td nowrap="">Overall design</td>

<td style="text-align: justify">ATAC-seq of SK-N-BE(2)c with and without ASO treatment (2 NESPR-targeting ASOs, 1 negative control ASO).<br></td>

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<tr valign="top"><td nowrap="">&nbsp;</td>

<td></td>

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<tr valign="top"><td>Contributor(s)</td>

<td><a href="/pubmed/?term=Delhaye L[Author]">Delhaye L</a>, <a href="/pubmed/?term=Mestdagh P[Author]">Mestdagh P</a>, <a href="/pubmed/?term=Roels J[Author]">Roels J</a>, <a href="/pubmed/?term=Van Vlierberghe P[Author]">Van Vlierberghe P</a></td>

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<tr valign="top"><td>Citation missing</td>

<td class="message" id="pmidCell">Has this study been published? Please <a href="/geo/submitter">login</a> to update or <a href="mailto:geo@ncbi.nlm.nih.gov?subject=Citation for GSE293716 [not logged in]&amp;body=Thank you very much for helping to keep GEO citation links up-to-date. Please enter the PubMed ID (PMID) or full citation for GSE293716 below, and we will update the record accordingly within the next day or two. The PubMed ID or citation is:

">notify GEO</a>.</td>

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<tr valign="top"><td colspan="2"><span id="geo2r"></span> <span id="rnaseq_counts"></span></td></tr>

<tr bgcolor="#eeeeee" valign="top"><td>Submission date</td>

<td>Apr 03, 2025</td>

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<tr bgcolor="#eeeeee" valign="top"><td>Last update date</td>

<td>Apr 03, 2025</td>

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<tr bgcolor="#eeeeee" valign="top"><td>Contact name</td>

<td>Louis Delhaye</td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">E-mail(s)</td>

<td><a href="mailto:louis.delhaye@ugent.be">louis.delhaye@ugent.be</a><br></td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Organization name</td>

<td style="text-align: justify">Ghent University<br></td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Street address</td>

<td style="text-align: justify">Corneel Heymanslaan 10<br></td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">City</td>

<td style="text-align: justify">Gent</td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">ZIP/Postal code</td>

<td style="text-align: justify">9000</td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Country</td>

<td style="text-align: justify">Belgium</td>

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<tr valign="top"><td nowrap="">&nbsp;</td>

<td></td>

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<tr valign="top"><td>Platforms (1)</td>

<td onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)"><table cellpadding="3" style="position:relative;top:-5px;left:-5px"><tbody><tr><td valign="top"><a href="/geo/query/acc.cgi?acc=GPL18573" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)">GPL18573</a></td>

<td valign="top">Illumina NextSeq 500 (Homo sapiens)</td>

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