--- a +++ b/test/test_project.yaml @@ -0,0 +1,54 @@ +project_name : "test" +project_dir : "test/test_project/" +sequencing_runs : + - name : "Run1" + version : 'v1' + dir : "test/seq_runs/run_v1_single_file" + fastq_path : "{read}.fastq.gz" + library_name : "test_lib0" + - name : "Run2" + version : 'v2' + dir : "test/seq_runs/run_v2_single_file" + fastq_path : "{read}.fastq.gz" + library_name : "test_lib1" + - name : "Run3" + version : 'v2' + dir : "test/seq_runs/run_v2_split" + fastq_path : "{library_prefix}_{split_affix}_{read}_001.fastq.gz" + split_affixes : ["L001", "L002"] + libraries : + - {library_name: "test_lib1", library_prefix: "lib1"} + - {library_name: "test_lib2", library_prefix: "lib2"} + - name : 'Run4' + version : 'v3' + dir : "test/seq_runs/run_v3" + fastq_path : "Undetermined_S0_{split_affix}_{read}_001.fastq.gz" + split_affixes : ["L001", "L002"] + libraries : + - {library_name: "test_lib3", library_index: "ATAGAG"} + - {library_name: "test_lib4", library_index: "AGAGGA"} +paths : + bowtie_index : "/Users/averes/Projects/Melton/grch38_test/Homo_sapiens.GRCh38.85.annotated" + python_dir : "/Users/averes/miniconda3/envs/py27/bin/" + samtools_dir : "/Users/averes/software/samtools-1.3.1/bin/" + rsem_dir : "" + java_dir : "" +parameters : # OPTIONAL PARAMETERS +# umi_quantification_arguments: +# m : 10 #Ignore reads with more than M alignments, after filtering on distance from transcript end. +# u : 1 #Ignore counts from UMI that should be split among more than U genes. +# d : 600 #Maximal distance from transcript end, NOT INCLUDING THE POLYA TAIL +# split-ambigs: False #If umi is assigned to m genes, add 1/m to each gene's count (instead of 1) +# min_non_polyA: 15 #Require reads to align to this much non-polyA sequence. (Set to 0 to disable filtering on this parameter.) +# output_arguments: +# output_unaligned_reads_to_other_fastq: False +# low_complexity_mask: False +# bowtie_arguments: +# m : 200 +# n : 1 +# l : 15 +# e : 1000 + trimmomatic_arguments: + LEADING: "28" + SLIDINGWINDOW: "4:20" + MINLEN: "16"