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a |
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b/default_parameters.yaml |
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1 |
paths : |
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bowtie_dir : '' |
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rsem_dir : '' |
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python_dir : '' |
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java_dir : '' |
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samtools_dir : '' |
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parameters : |
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umi_quantification_arguments: |
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m : 10 #Ignore reads with more than M alignments, after filtering on distance from transcript end. |
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u : 1 #Ignore counts from UMI that should be split among more than U genes. |
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d : 600 #Maximal distance from transcript end, NOT INCLUDING THE POLYA TAIL |
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split-ambigs: False #If umi is assigned to m genes, add 1/m to each gene's count (instead of 1) |
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min_non_polyA: 15 #Require reads to align to this much non-polyA sequence. (Set to 0 to disable filtering on this parameter.) |
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output_arguments: |
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output_unaligned_reads_to_other_fastq: False |
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filter_alignments_to_softmasked_regions: False |
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bowtie_arguments: |
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m : 200 |
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n : 1 |
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l : 15 |
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e : 80 |
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trimmomatic_arguments: |
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LEADING: "28" |
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SLIDINGWINDOW: "4:20" |
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MINLEN: "16" |
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argument_order: ['LEADING', 'SLIDINGWINDOW', 'MINLEN'] |
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low_complexity_filter_arguments: |
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max_low_complexity_fraction : 0.50 |