--- a +++ b/GSE155114_README.txt @@ -0,0 +1,210 @@ +This file describes the files for the single-nucleus RNA sequencing analysis of human chronic traumatic encephalopathy (CTE) tissue and matched control tissue. + +Sixteen samples were collected via the inDrops single cell method, 8 CTE and 8 controls. The inDrops method separates samples into groups of ~3,000 nuclei, which is why each samples is further divided, e.g. Control1.1, Control1.2, Control1.3. + +Complementary DNA produced by the inDrops facility was sequenced over eight NextSeq500 runs: Run1, Run2, Run3.1, Run3.2, Run4.1, Run4.2, Run5.1, Run5.2. Each run produced several count matrices comprising both control and CTE data. Count matrices are combined for each run and named Sample1.tsv Sample2.tsv, Sample3.tsv, Sample4.tsv, Sample5.tsv, Sample6.tsv, Sample7.tsv, Sample 8.tsv. + +Data are organized as follows: +Run1 produced data in Sample1 +Sample 1 contains: +control.5.1 +control.5.2 +control.5.3 +CTE.1.1 +CTE.1.2 +CTE.1.3 + +Run2 produced data in Sample2 +Sample 2 contains: +control.4.1 +control.4.2 +control.6.1 +control.6.2 +control.6.3 +control.7.1 +control.7.2 +control.7.3 +CTE.2.1 +CTE.2.2 +CTE.3.1 +CTE.3.2 +CTE.4.1 +CTE.4.2 +CTE.4.3 + +Run3.1 produced data in Sample3 +Sample3 contains: +control.5.4 +control.5.5 +control.5.6 +control.6.4 +control.6.5 +control.6.6 +CTE.2.3 +CTE.2.4 +CTE.2.5 +CTE.3.3 +CTE.3.4 +CTE.3.5 + +Run3.2 produced data in Sample4 +Sample4 contains: +control.4.3 +control.4.4 +control.4.5 +control.7.4 +control.7.5 +control.7.6 +CTE.1.4 +CTE.1.5 +CTE.1.6 +CTE.4.4 +CTE.4.5 +CTE.4.6 + +Run4.1 produced data in Sample5 +Sample5 contains: +control.2.1 +control.2.2 +control.2.3 +control.8.1 +control.8.2 +control.8.3 +CTE.5.1 +CTE.5.2 +CTE.5.3 +CTE.6.1 +CTE.6.2 +CTE.6.3 + +Run4.2 produced data in Sample6 +Sample6 contains: +control.1.1 +control.1.2 +control.1.3 +control.3.1 +control.3.2 +control.3.3 +CTE.7.1 +CTE.7.2 +CTE.7.3 +CTE.8.1 +CTE.8.2 +CTE.8.3 + +Run5.1 produced data in Sample7 +Sample7 contains: +control.5.7 +control.5.8 +control.5.9 +control.7.7 +control.7.8 +control.7.9 + +Run5.2 produced data in Sample9 +Sample9 contains: +control.2.4 +control.2.5 +control.2.6 +control.8.4 +control.8.5 +control.8.6 + +Fastq files can be de-multiplexed to reproduce count matrices following the inDrops processing pipeline: https://github.com/indrops/indrops +Below are the library indices needed as input in the inDrops processing pipeline for each sample. +Sample1 +control.5.1 TCGCATAA +control.5.2 TTACCTCC +control.5.3 CAGTTATG +CTE.1.1 CTTAATAG +CTE.1.2 ATAGCCTT +CTE.1.3 TAAGGCTC + +Sample2 +control.4.1 AGAGGATA +control.4.2 CTCCTTAC +control.4.3 TATGCAGT +control.6.1 TACTCCTT +control.6.2 AGGCTTAG +control.6.3 ATTAGACG +control.7.1 ACGCTCTT +control.7.2 CGCATTCT +control.7.3 ATAGAGAG +CTE.2.1 CGGAGAGA +CTE.2.2 AGCTAGAA +CTE.3.1 TCTTACGC +CTE.3.2 CTTAATAG +CTE.4.1 CCTTTACT +CTE.4.2 GAGACGGA +CTE.4.3 AGAAAGCT + +Sample3 +control.5.4 ATAGAGAG +control.5.5 AGAGGATA +control.5.6 CTCCTTAC +control.6.4 AGCTAGAA +control.6.5 ACTCTAGG +control.6.6 TCTTACGC +CTE.2.3 ATTAGACG +CTE.2.4 CGGAGAGA +CTE.2.5 CTAGTCGA +CTE.3.3 TATGCAGT +CTE.3.4 TACTCCTT +CTE.3.5 AGGCTTAG + +Sample4 +control.4.4 ACGCTCTT +control.4.5 CGCATTCT +control.7.4 CCTTTACT +control.7.5 GAGACGGA +control.7.6 AGAAAGCT +CTE.1.4 TCGCATAA +CTE.1.5 TTACCTCC +CTE.1.6 CAGTTATG +CTE.4.4 CTTAATAG +CTE.4.5 ATAGCCTT +CTE.4.6 TAAGGCTC + +Sample5 +control.2.1 TATGCAGT +control.2.2 TACTCCTT +control.2.3 AGGCTTAG +control.8.1 ATAGAGAG +control.8.2 AGAGGATA +control.8.3 CTCCTTAC +CTE.5.1 ATTAGACG +CTE.5.2 CGGAGAGA +CTE.5.3 CTAGTCGA +CTE.6.1 AGCTAGAA +CTE.6.2 ACTCTAGG +CTE.6.3 TCTTACGC + +Sample6 +control.1.1 CTTAATAG +control.1.2 ATAGCCTT +control.1.3 TAAGGCTC +control.3.1 TCGCATAA +control.3.2 TTACCTCC +control.3.3 CAGTTATG +CTE.7.1 CCTTTACT +CTE.7.2 GAGACGGA +CTE.7.3 AGAAAGCT +CTE.8.1 ACGCTCTT +CTE.8.2 CGCATTCT +CTE.8.3 GGTACTCA + +Sample7 +control.5.7 ATAGAGAG +control.5.8 AGAGGATA +control.5.9 CTCCTTAC +control.7.7 TATGCAGT +control.7.8 TACTCCTT +control.7.9 AGGCTTAG + +Sample8 +control.2.4 ACGCTCTT +control.2.5 CGCATTCT +control.2.6 GGTACTCA +control.8.4 CCTTTACT +control.8.5 GAGACGGA +control.8.6 AGAAAGCT