<table><tbody><tr><td><table cellpadding="2" cellspacing="0" width="600"><tbody><tr bgcolor="#cccccc" valign="top"><td colspan="2"><table width="600"><tbody><tr><td><strong class="acc" id="GSE280537"><a href="/geo/query/acc.cgi?acc=GSE280537" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)">Series GSE280537</a></strong></td>

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<td align="right" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_gds)"><a href="/gds/?term=GSE280537[Accession]">Query DataSets for GSE280537</a></td>

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<tr valign="top"><td>Status</td>

<td>Public on Nov 10, 2024</td>

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<tr valign="top"><td nowrap="">Title</td>

<td style="text-align: justify">Single Cell RNA Sequencing of Synovial Tissue in Adolescents Undergoing ACL Reconstruction</td>

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<tr valign="top"><td nowrap="">Organism</td>

<td><a href="/Taxonomy/Browser/wwwtax.cgi?mode=Info&amp;id=9606" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_organismus)">Homo sapiens</a></td>

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<tr valign="top"><td nowrap="">Experiment type</td>

<td>Expression profiling by high throughput sequencing<br></td>

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<tr valign="top"><td nowrap="">Summary</td>

<td style="text-align: justify">Background: Loss of motion and arthrofibrosis after anterior cruciate ligament reconstruction (ACLR) can be a devastating complication for athletes. The cellular and molecular pathogenesis of arthrofibrosis is poorly understood, limiting prevention and treatment options. Synovial inflammation may contribute to post-ACLR arthrofibrosis. Hypothesis/Purpose: We hypothesized that higher synovial immune cell infiltration and inflammatory/catabolic gene expression patterns at the time of ACLR would correlate with poorer motion-related outcomes. Study Design: Case Series Methods: Patients aged 10-18 undergoing primary ACLR were enrolled in a prospective pilot study, and synovial tissue biopsies were obtained during ACLR. Flow cytometry and single cell RNA-sequencing explored synovial cell types/frequencies and gene expression. Principle component analysis followed by clustering grouped patients into distinct immunophenotypes based on their synovial cell composition. Clinical follow-up with knee range of motion (ROM), need for lysis of adhesions, and patient reported outcome measures were collected and compared between immunophenotypes. Results: Enrolled patients (n = 17) underwent ACLR at a median of 37 days post-injury. Analysis revealed three distinct immunophenotypes. Type 1 comprised patients with the longest time between injury and surgery and the lowest hematopoietic and T cell infiltration. Types 2 and 3 had similar times between injury and surgery, and Type 2 had intermediate while Type 3 had the highest hematopoietic and T cell percentages. Type 3 was associated with worse ROM at 2- and 6-weeks post-op; T cell prevalence and ROM were inversely correlated at those time points. The only patient requiring lysis of adhesions for arthrofibrosis was in Type 3. Conclusion: Synovial immune infiltration after ACL injury shows variability between patients that clusters into three immunophenotypes correlating with early ROM and the risk of arthrofibrosis. T cell recruitment and infiltration was the strongest factor correlated with ROM outcomes and presents an exciting venue for future research on post-ACLR arthrofibrosis.<br></td>

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<tr valign="top"><td nowrap="">Overall design</td>

<td style="text-align: justify">Synovial tissue biopsies were obtained during ACL reconstruction from six patients, enzymatically digested to obtain heterogenous single-cell suspensions, and analyzed by scRNA-seq. The six scRNA-seq samples as well as eleven other patient samples were categorized into three Immunotypes based on flow cytometry quantification of the immune cells present in the synovium tissue. The scRNA-seq output was analyzed for a more comprehensive characterization of the cell types present as well as the relative expression of select inflammatory markers and immunomodulatory genes.<br></td>

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<tr valign="top"><td nowrap="">Web link</td>

<td><a href="https://pubmed.ncbi.nlm.nih.gov/39772756/">https://pubmed.ncbi.nlm.nih.gov/39772756/</a><br></td>

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<tr valign="top"><td>Contributor(s)</td>

<td><a href="/pubmed/?term=Sarah R[Author]">Sarah R</a>, <a href="/pubmed/?term=Guangxu J[Author]">Guangxu J</a>, <a href="/pubmed/?term=Nury S[Author]">Nury S</a>, <a href="/pubmed/?term=Bailey F[Author]">Bailey F</a>, <a href="/pubmed/?term=Jonathan R[Author]">Jonathan R</a></td>

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<tr valign="top"><td nowrap="">Citation(s)</td>

<td><span class="pubmed_id" id="39772756"><!--?xml version="1.0" encoding="UTF-8"?-->

<div class="PubmedCitation"><ul><li><span class="authors">Romereim SM, Smykowski MR, Ball EK, Carey EG et al. </span><span class="title">Immunophenotyping of Synovial Tissue in Adolescents Undergoing ACL Reconstruction: What Is the Role of Synovial Inflammation in Arthrofibrosis? </span><span class="source">Am J Sports Med</span> 2025 Feb;53(2):315-326. PMID:&nbsp;<a title="Link to PubMed record" href="https://www.ncbi.nlm.nih.gov/pubmed/39772756">39772756</a></li></ul></div>

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<tr valign="top"><td colspan="2"><span id="geo2r"></span> <span id="rnaseq_counts"></span></td></tr>

<tr bgcolor="#eeeeee" valign="top"><td>Submission date</td>

<td>Oct 29, 2024</td>

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<tr bgcolor="#eeeeee" valign="top"><td>Last update date</td>

<td>Feb 12, 2025</td>

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<tr bgcolor="#eeeeee" valign="top"><td>Contact name</td>

<td>Sarah Romereim</td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Organization name</td>

<td style="text-align: justify">Atrium Health<br></td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Department</td>

<td style="text-align: justify">Musculoskeletal Institute<br></td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Street address</td>

<td style="text-align: justify">1000 Blythe Blvd, CRC 305<br></td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">City</td>

<td style="text-align: justify">Charlotte</td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">State/province</td>

<td style="text-align: justify">NC</td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">ZIP/Postal code</td>

<td style="text-align: justify">28203</td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Country</td>

<td style="text-align: justify">USA</td>

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<tr valign="top"><td nowrap="">&nbsp;</td>

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