<table><tbody><tr><td><table cellpadding="2" cellspacing="0" width="600"><tbody><tr bgcolor="#cccccc" valign="top"><td colspan="2"><table width="600"><tbody><tr><td><strong class="acc" id="GSE246234"><a href="/geo/query/acc.cgi?acc=GSE246234" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)">Series GSE246234</a></strong></td>

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<td align="right" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_gds)"><a href="/gds/?term=GSE246234[Accession]">Query DataSets for GSE246234</a></td>

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<tr valign="top"><td>Status</td>

<td>Public on Mar 05, 2025</td>

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<tr valign="top"><td nowrap="">Title</td>

<td style="text-align: justify">RNA sequencing (RNA-seq) of LAIR-1 overexpression in SKOV-3 ovarian cancer cell lines</td>

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<tr valign="top"><td nowrap="">Organism</td>

<td><a href="/Taxonomy/Browser/wwwtax.cgi?mode=Info&amp;id=9606" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_organismus)">Homo sapiens</a></td>

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<tr valign="top"><td nowrap="">Experiment type</td>

<td>Expression profiling by high throughput sequencing<br></td>

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<tr valign="top"><td nowrap="">Summary</td>

<td style="text-align: justify">Leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1), is widely expressed by solid tumor cells and functions as a tumor suppressor. Our previous studies have found that LAIR-1 is expressed in ovarian cancer tumor tissues and in a variety of epithelial ovarian cancer cell lines and that LAIR-1 could inhibit ovarian cancer cell growth by suppressing the PI3K-AKT-mTOR pathway. The aim of the present study was to obtain a comprehensive understanding of the molecular mechanism of LAIR-1 in regulating the biological behavior of ovarian cancer cells. The approach taken was to perform RNA-seq sequencing on SKOV3 cells overexpressing LAIR-1, and to conduct further validation by combinatorial integration of bioinformatics analyses to assess the genetic alterations and using biological experimental findings.<br></td>

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<tr valign="top"><td nowrap="">&nbsp;</td>

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<tr valign="top"><td nowrap="">Overall design</td>

<td style="text-align: justify">There are two groups cells used for this expression profile comparison : NC (negative control) and OE(LAIR-1–overexpression) . Total RNA was extracted with Trizol, 1 μg of total RNA was hybridized to the probe and digested with RNase H followed by DNase I. RNA was purified using VAHTS RNA purification beads, 18.5 μL Frag/Prime Buffer (1x) was added, mixed by pipetting, and allowed to stand for 2 minutes at room temperature. After standing on a magnetic stand for 5 minutes to allow the solution to clear, 16 μL of supernatant was gently aspirated into a new nuclease-free centrifuge tube, and the samples were fragmented using a PCR instrument and sequenced using an Illumina sequencer.<br></td>

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<tr valign="top"><td nowrap="">Citation(s)</td>

<td><span class="pubmed_id" id="40000955"><!--?xml version="1.0" encoding="UTF-8"?-->

<div class="PubmedCitation"><ul><li><span class="authors">Zhang H, Liu M, Zhong H, Ma L et al. </span><span class="title">Mechanistic role of FN1 in LAIR-1 mediated downregulation of ovarian cancer cell proliferation. </span><span class="source">BMC Cancer</span> 2025 Feb 25;25(1):339. PMID:&nbsp;<a title="Link to PubMed record" href="https://www.ncbi.nlm.nih.gov/pubmed/40000955">40000955</a></li></ul></div>

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<tr valign="top"><td colspan="2"><span id="geo2r"></span> <span id="rnaseq_counts"></span></td></tr>

<tr bgcolor="#eeeeee" valign="top"><td>Submission date</td>

<td>Oct 25, 2023</td>

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<tr bgcolor="#eeeeee" valign="top"><td>Last update date</td>

<td>Mar 05, 2025</td>

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<tr bgcolor="#eeeeee" valign="top"><td>Contact name</td>

<td>Huachang zhang</td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">E-mail(s)</td>

<td><a href="mailto:zhc1822001852@126.com">zhc1822001852@126.com</a><br></td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Organization name</td>

<td style="text-align: justify">Binzou medical university<br></td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Street address</td>

<td style="text-align: justify">346 Guanhai Road, Laishan District<br></td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">City</td>

<td style="text-align: justify">Yantai</td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">State/province</td>

<td style="text-align: justify">Shandong</td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">ZIP/Postal code</td>

<td style="text-align: justify">264003</td>

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<tr bgcolor="#eeeeee" valign="top"><td nowrap="">Country</td>

<td style="text-align: justify">China</td>

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<tr valign="top"><td nowrap="">&nbsp;</td>

<td></td>

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<tr valign="top"><td>Platforms (1)</td>

<td onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)"><table cellpadding="3" style="position:relative;top:-5px;left:-5px"><tbody><tr><td valign="top"><a href="/geo/query/acc.cgi?acc=GPL24676" onmouseout="onLinkOut('HelpMessage' , geo_empty_help)" onmouseover="onLinkOver('HelpMessage' , geoaxema_recenter)">GPL24676</a></td>

<td valign="top">Illumina NovaSeq 6000 (Homo sapiens)</td>

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