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| Status |
Public on Mar 05, 2025 |
| Title |
RNA sequencing (RNA-seq) of LAIR-1 overexpression in SKOV-3 ovarian cancer cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1), is widely expressed by solid tumor cells and functions as a tumor suppressor. Our previous studies have found that LAIR-1 is expressed in ovarian cancer tumor tissues and in a variety of epithelial ovarian cancer cell lines and that LAIR-1 could inhibit ovarian cancer cell growth by suppressing the PI3K-AKT-mTOR pathway. The aim of the present study was to obtain a comprehensive understanding of the molecular mechanism of LAIR-1 in regulating the biological behavior of ovarian cancer cells. The approach taken was to perform RNA-seq sequencing on SKOV3 cells overexpressing LAIR-1, and to conduct further validation by combinatorial integration of bioinformatics analyses to assess the genetic alterations and using biological experimental findings.
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| Overall design |
There are two groups cells used for this expression profile comparison : NC (negative control) and OE(LAIR-1–overexpression) . Total RNA was extracted with Trizol, 1 μg of total RNA was hybridized to the probe and digested with RNase H followed by DNase I. RNA was purified using VAHTS RNA purification beads, 18.5 μL Frag/Prime Buffer (1x) was added, mixed by pipetting, and allowed to stand for 2 minutes at room temperature. After standing on a magnetic stand for 5 minutes to allow the solution to clear, 16 μL of supernatant was gently aspirated into a new nuclease-free centrifuge tube, and the samples were fragmented using a PCR instrument and sequenced using an Illumina sequencer.
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| Citation(s) |
- Zhang H, Liu M, Zhong H, Ma L et al. Mechanistic role of FN1 in LAIR-1 mediated downregulation of ovarian cancer cell proliferation. BMC Cancer 2025 Feb 25;25(1):339. PMID: 40000955
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| Submission date |
Oct 25, 2023 |
| Last update date |
Mar 05, 2025 |
| Contact name |
Huachang zhang |
| E-mail(s) |
zhc1822001852@126.com
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| Organization name |
Binzou medical university
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| Street address |
346 Guanhai Road, Laishan District
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| City |
Yantai |
| State/province |
Shandong |
| ZIP/Postal code |
264003 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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