|
| Status |
Public on Apr 02, 2025 |
| Title |
Gene expression in small cell carcinoma of the ovary, hypercalcemic type (SCCOHT) cell line COV434 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Small cell carcinoma of the ovary, hypercalcemic type (SCCOHT) is a rare and aggressive form of ovarian cancer. SCCOHT tumors have inactivating mutations in SMARCA4 (BRG1), one of the two mutually exclusive ATPases of the SWI/SNF chromatin remodeling complex. To address the role that SMARCA4 loss plays in SCCOHT tumorigenesis, we performed RNA-seq in a SCCOHT cell line +/- SMARCA4 re-expression. SMARCA4 re-expression was acheived by integration of pIND20-BRG1 and inducing with doxycycline. The goal of this study was to generate a high sequencing depth RNA-seq dataset to determine how restoration of SMARCA4 results in changes in gene expression and RNA splicing.
|
| |
|
| Overall design |
COV434 pIND20 BRG1-2.1 and parental COV434 cell lines were induced as previously described (PMID:29440177) with and without doxycycline to re-express SMARCA4. Experiment was repeated in independent triplicate.
|
| |
|
| Contributor(s) |
Jessica L, Elizabeth R, Patrick P, Jeffrey T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 10, 2022 |
| Last update date |
Apr 03, 2025 |
| Contact name |
Jessica Diane Lang |
| E-mail(s) |
jessicadianelang@gmail.com
|
| Phone |
6082628601
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Pathology and Laboratory Medicine
|
| Street address |
1111 HIGHLAND AVE WIMR 2766
|
| City |
Madison |
| State/province |
Wisconsin |
| ZIP/Postal code |
53705 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
