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| Status |
Public on Apr 03, 2025 |
| Title |
Peritoneal tumor spread in serous ovarian cancer-epithelial mesenchymal status and outcome |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study we aimed to analyze the biological mechanisms underlying apparently different modes of peritoneal tumor spread in serous ovarian cancer: miliary (widespread, millet-like lesions) versus non-miliary (bigger, exophytically growing implants). Tumor tissues and ascites from 23 chemotherapy naive patients were analyzed by RNA-sequencing and flow cytometry. On the basis of differential gene expression between miliary and non-miliary, gene signatures were developed. A calculated tumor spread factor revealed a significant independent negative impact of miliary spread on overall survival (HR 3.77; CI95 1.14–12.39; p = 0.029) in an independent cohort of 165 serous ovarian cancer patients. Comparing previously published epithelial-mesenchymal transition (EMT) gene signatures, non-miliary spread correlated significantly with a reduced epithelial status. We conclude that serous ovarian cancer is a heterogeneous disease with distinct modes of peritoneal tumor spread, differing not only in clinical appearance, but also in molecular characteristics and outcome. EMT, peritoneal inflammation status, and therapeutic options are discussed. Significance: More than half of serous epithelial ovarian cancer patients present with a newly described type of intraperitoneal tumor spread, associated with differences in the inflammation status, activated oncogenic pathways, lack of EMT, and thus reduced overall survival. Both, the diminished immune reaction and the enhanced epithelial and malignant characteristics of the tumor cells open new avenues for therapeutic options and strategies, like Catumaxomab, already in clinical use.
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| Overall design |
From 42 EpCAM-enriched tumor cells of High-Grade Serous Ovarian cancer patients total RNA was isolated and RNA-sequencing performed.
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| Contributor(s) |
Pils D |
| Citation(s) |
- Auer K, Bachmayr-Heyda A, Aust S, Sukhbaatar N et al. Peritoneal tumor spread in serous ovarian cancer-epithelial mesenchymal status and outcome. Oncotarget 2015 Jul 10;6(19):17261-75. PMID: 25991672
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| Submission date |
Apr 02, 2025 |
| Last update date |
Apr 03, 2025 |
| Contact name |
Dietmar Pils |
| E-mail(s) |
dietmar.pils@meduniwien.ac.at
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| Organization name |
Medical Univ. of Vienna
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| Department |
Dept. of General Surgery
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| Lab |
PILS
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| Street address |
Spitalgasse 23
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| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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